Restriction map for pF1KB7226
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BclI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspEI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          47  CGCG                 CG^CG
AlwNI         763  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          736  CCTGAGG              CC^T[ACGT]AGG
BamHI           8  GGATCC               G^GATCC
BanI          601  GGCACC               G^G[CT][GA]CC
BanII        1267  GGGCTC               G[GA]GC[CT]^C
BciVI         837  GGATAC               GTATCC(6/5)
BlpI         1309  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1309  GCTGAGC              GC^T[ACGT]AGC
BseRI         373  GAGGAG               GAGGAG(10/8)
BsmAI         376  GAGAC                GTCTC(1/5)
BsmI         1329  GAATGC               GAATGC(1/-1)
BspHI          89  TCATGA               T^CATGA
BspMI         440  ACCTGC               ACCTGC(4/8)
BsrFI         356  ACCGGC               [GA]^CCGG[CT]
BssSI        1105  CACGAG               CACGAG(-5/-1)
BstUI          47  CGCG                 CG^CG
BstYI           8  GGATCC               [GA]^GATC[CT]
Bsu36I        736  CCTGAGG              CC^T[ACGT]AGG
CelII        1309  GCTGAGC              GC^T[ACGT]AGC
CfoI          107  GCGC                 GCG^C
Cfr10I        356  ACCGGC               [GA]^CCGG[CT]
DraII         722  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          110  CCATGG               C^C[GA][CT]GG
Eco81I        736  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      722  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HaeII         106  AGCGCC               [GA]GCGC^[CT]
HhaI          107  GCGC                 GCG^C
HinP1I        107  GCGC                 G^CGC
HincII        469  GTCAAC               GT[CT]^[GA]AC
HindII        469  GTCAAC               GT[CT]^[GA]AC
HindIII       703  AAGCTT               A^AGCTT
HinfI         120  GAGTC                G^A[ACGT]TC
MaeII         730  ACGT                 A^CGT
MflI            8  GGATCC               [GA]^GATC[CT]
MseI         1198  TTAA                 T^TAA
MvnI           47  CGCG                 CG^CG
NcoI          110  CCATGG               C^CATGG
NspI          452  ACATGC               [GA]CATG^[CT]
PleI          120  GAGTC                GAGTC(4/5)
PshAI         661  GACTTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI           89  TCATGA               T^CATGA
ScaI         1094  AGTACT               AGT^ACT
SexAI          95  ACCAGGT              A^CC[AT]GGT
StuI          381  AGGCCT               AGG^CCT
Tru9I        1198  TTAA                 T^TAA
XcmI          869  CCACCAAGACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII           8  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          244  GTAC                 GT^AC
             1095  GTAC                
Alw44I       1072  GTGCAC               G^TGCAC
             1102  GTGCAC              
AlwI            9  GATCC                GGATC(4/5)
                8  GGATC               
ApaLI        1072  GTGCAC               G^TGCAC
             1102  GTGCAC              
AspHI        1072  GTGCAC               G[AT]GC[AT]^C
             1102  GTGCAC              
BalI         1124  TGGCCA               TGG^CCA
             1289  TGGCCA              
BbsI          432  GAAGAC               GAAGAC(2/6)
              975  GAAGAC              
BfaI          446  CTAG                 C^TAG
             1066  CTAG                
BglI          593  GCCAGATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              715  GCCGGGGAGGC         
BpuAI         432  GAAGAC               GAAGAC(2/6)
              975  GAAGAC              
BsiHKAI      1072  GTGCAC               G[AT]GC[AT]^C
             1102  GTGCAC              
BspDI         529  ATCGAT               AT^CGAT
             1170  ATCGAT              
BstAPI        762  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              793  GCAGAGCTTGC         
ClaI          529  ATCGAT               AT^CGAT
             1170  ATCGAT              
EaeI         1124  TGGCCA               [CT]^GGCC[GA]
             1289  TGGCCA              
EcoT14I       110  CCATGG               C^C[AT][AT]GG
              962  CCAAGG              
FauI          523  CCCGC                CCCGC(4/6)
              366  GCGGG               
MaeI          446  CTAG                 C^TAG
             1066  CTAG                
MluNI        1124  TGGCCA               TGG^CCA
             1289  TGGCCA              
MscI         1124  TGGCCA               TGG^CCA
             1289  TGGCCA              
MslI          276  CACAGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              582  CATGCCTGTG          
RsaI          244  GTAC                 GT^AC
             1095  GTAC                
StyI          110  CCATGG               C^C[AT][AT]GG
              962  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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