Restriction map for pF1KB7226
 (Linear) (Six-base) MAPSORT of: KIBB7226.seq  Check: 1693  from: 1  to: 1359



KIBB7226.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     648     917    1105    1359 
   Size:        648     269     188     254

  Fragments arranged by size: 

                 648     269     254     188
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1006    1359 
   Size:       1006     353
 
AlwNI CAG_nnn'CTG

Cuts at:      0     768    1359 
   Size:        768     591
 
ApaLI G'TGCA_C

Cuts at:      0    1072    1102    1359 
   Size:       1072      30     257

  Fragments arranged by size: 

                1072     257      30
 
BamHI G'GATC_C

Cuts at:      0       8    1359 
   Size:          8    1351
 
BanI G'GyrC_C

Cuts at:      0     601    1359 
   Size:        601     758
 
BanII G_rGCy'C

Cuts at:      0    1271    1359 
   Size:       1271      88
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     439     982    1359 
   Size:        439     543     377

  Fragments arranged by size: 

                 543     439     377
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     831    1359 
   Size:        831     528
 
BglI GCCn_nnn'nGGC

Cuts at:      0     599     721    1359 
   Size:        599     122     638

  Fragments arranged by size: 

                 638     599     122
 
BlpI GC'TnA_GC

Cuts at:      0    1310    1359 
   Size:       1310      49
 
Bme1580I G_kGCm'C

Cuts at:      0      66     604    1076    1106    1359 
   Size:         66     538     472      30     253

  Fragments arranged by size: 

                 538     472     253      66      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1234    1359 
   Size:       1234     125
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1061    1359 
   Size:       1061     298
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     269    1033    1359 
   Size:        151     118     764     326

  Fragments arranged by size: 

                 764     326     151     118
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     388    1359 
   Size:        388     971
 
BseYI C'CCAG_C

Cuts at:      0     774    1353    1359 
   Size:        774     579       6

  Fragments arranged by size: 

                 774     579       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     697     979    1359 
   Size:        478     219     282     380

  Fragments arranged by size: 

                 478     380     282     219
 
BsiHKAI G_wGCw'C

Cuts at:      0    1076    1106    1359 
   Size:       1076      30     253

  Fragments arranged by size: 

                1076     253      30
 
BsmI GAATG_Cn'

Cuts at:      0    1335    1359 
   Size:       1335      24
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     604    1076    1106    1271    1359 
   Size:         66     538     472      30     165      88

  Fragments arranged by size: 

                 538     472     165      88      66      30
 
BspHI T'CATG_A

Cuts at:      0      89    1359 
   Size:         89    1270
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     449    1359 
   Size:        449     910
 
BsrFI r'CCGG_y

Cuts at:      0     356    1359 
   Size:        356    1003
 
BssSI C'ACGA_G

Cuts at:      0    1105    1359 
   Size:       1105     254
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     768     799    1359 
   Size:        768      31     560

  Fragments arranged by size: 

                 768     560      31
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     588     879    1134    1359 
   Size:        588     291     255     225

  Fragments arranged by size: 

                 588     291     255     225
 
BstYI r'GATC_y

Cuts at:      0       8    1359 
   Size:          8    1351
 
Bsu36I CC'TnA_GG

Cuts at:      0     737    1359 
   Size:        737     622
 
BtgI C'CryG_G

Cuts at:      0     110    1359 
   Size:        110    1249
 
BtsI GCAGTG_nn'

Cuts at:      0      64    1359 
   Size:         64    1295
 
ClaI AT'CG_AT

Cuts at:      0     530    1171    1359 
   Size:        530     641     188

  Fragments arranged by size: 

                 641     530     188
 
EaeI y'GGCC_r

Cuts at:      0    1124    1289    1359 
   Size:       1124     165      70

  Fragments arranged by size: 

                1124     165      70
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1050    1359 
   Size:       1050     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     269     648     917    1033    1105    1359 
   Size:        151     118     379     269     116      72     254

  Fragments arranged by size: 

                 379     269     254     151     118     116      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0     723    1359 
   Size:        723     636
 
HaeII r_GCGC'y

Cuts at:      0     110    1359 
   Size:        110    1249
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     242     274     867     899    1359 
   Size:        242      32     593      32     460

  Fragments arranged by size: 

                 593     460     242      32      32
 
HincII GTy'rAC

Cuts at:      0     471    1359 
   Size:        471     888
 
HindIII A'AGCT_T

Cuts at:      0     703    1359 
   Size:        703     656
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     678    1359 
   Size:        678     681
 
MscI TGG'CCA

Cuts at:      0    1126    1291    1359 
   Size:       1126     165      68

  Fragments arranged by size: 

                1126     165      68
 
MslI CAynn'nnrTG

Cuts at:      0     280     586    1359 
   Size:        280     306     773

  Fragments arranged by size: 

                 773     306     280
 
MspA1I CmG'CkG

Cuts at:      0     219     765    1116    1359 
   Size:        219     546     351     243

  Fragments arranged by size: 

                 546     351     243     219
 
NcoI C'CATG_G

Cuts at:      0     110    1359 
   Size:        110    1249
 
NspI r_CATG'y

Cuts at:      0     456    1359 
   Size:        456     903
 
PasI CC'CwG_GG

Cuts at:      0      58     508    1359 
   Size:         58     450     851

  Fragments arranged by size: 

                 851     450      58
 
PfoI T'CCnGG_A

Cuts at:      0     128     479     833    1156    1277    1359 
   Size:        128     351     354     323     121      82

  Fragments arranged by size: 

                 354     351     323     128     121      82
 
PshAI GACnn'nnGTC

Cuts at:      0     665    1359 
   Size:        665     694
 
PstI C_TGCA'G

Cuts at:      0      31     795     812    1121    1359 
   Size:         31     764      17     309     238

  Fragments arranged by size: 

                 764     309     238      31      17
 
PvuII CAG'CTG

Cuts at:      0     219     765    1116    1359 
   Size:        219     546     351     243

  Fragments arranged by size: 

                 546     351     243     219
 
ScaI AGT'ACT

Cuts at:      0    1096    1359 
   Size:       1096     263
 
SexAI A'CCwGG_T

Cuts at:      0      95    1359 
   Size:         95    1264
 
SfcI C'TryA_G

Cuts at:      0      27     791     808     905    1117    1359 
   Size:         27     764      17      97     212     242

  Fragments arranged by size: 

                 764     242     212      97      27      17
 
StuI AGG'CCT

Cuts at:      0     383    1359 
   Size:        383     976
 
StyI C'CwwG_G

Cuts at:      0     110     962    1359 
   Size:        110     852     397

  Fragments arranged by size: 

                 852     397     110
 
TatI w'GTAC_w

Cuts at:      0    1094    1359 
   Size:       1094     265
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     876    1359 
   Size:        876     483
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    ApaLI    BamHI     BanI    BanII     BbsI
    BciVI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrFI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI
     EaeI     EciI  Eco57MI EcoO109I    HaeII    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PasI     PfoI
    PshAI     PstI    PvuII     ScaI    SexAI     SfcI     StuI     StyI
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI     BcgI     BclI
    BfrBI    BglII    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrGI   BssHII    BstBI   BstEII  BstZ17I    BtgZI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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