Restriction map for pF1KB7225
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        ItaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TseI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI          742  GAATTT               [GA]^AATT[CT]
ApaI          470  GGGCCC               GGGCC^C
ApoI          742  GAATTT               [GA]^AATT[CT]
AseI          112  ATTAAT               AT^TAAT
AsnI          112  ATTAAT               AT^TAAT
AvaI          443  CTCGAG               C^[CT]CG[GA]G
AvaII         510  GGACC                G^G[AT]CC
BanII         470  GGGCCC               G[GA]GC[CT]^C
BmyI          470  GGGCCC               G[AGT]GC[ACT]^C
BsaJI         698  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI         479  TCCGGT               [AT]^CCGG[AT]
BsmI          595  GAATGC               GAATGC(1/-1)
BsoBI         443  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      470  GGGCCC               G[AGT]GC[ACT]^C
Bsp1407I      667  TGTACA               T^GTACA
BsrDI         105  CATTGC               GCAATG(2/0)
BsrGI         667  TGTACA               T^GTACA
BssKI         297  CCAGG                ^CC[ACGT]GG
BstNI         297  CCAGG                CC^[AT]GG
BstYI         685  AGATCC               [GA]^GATC[CT]
Cac8I         607  GCCAGC               GC[ACGT]^[ACGT]GC
DdeI          263  CTGAG                C^T[ACGT]AG
EarI           25  CTCTTC               CTCTTC(1/4)
EcoRII        297  CCAGG                ^CC[AT]GG
EcoT14I       698  CCAAGG               C^C[AT][AT]GG
HapII         480  CCGG                 C^CGG
HincII        414  GTTGAC               GT[CT]^[GA]AC
HindII        414  GTTGAC               GT[CT]^[GA]AC
HpaII         480  CCGG                 C^CGG
HphI           18  GGTGA                GGTGA(8/7)
Ksp632I        25  CTCTTC               CTCTTC(1/4)
MaeII         633  ACGT                 A^CGT
MflI          685  AGATCC               [GA]^GATC[CT]
MspA1I        609  CAGCTG               C[AC]G^C[GT]G
MspI          480  CCGG                 C^CGG
MvaI          297  CCAGG                CC^[AT]GG
MwoI           39  GCTTCTCTCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          290  CATATG               CA^TATG
PaeR7I        443  CTCGAG               C^TCGAG
PleI          637  GACTC                GAGTC(4/5)
PshBI         112  ATTAAT               AT^TAAT
PvuII         609  CAGCTG               CAG^CTG
ScaI          266  AGTACT               AGT^ACT
ScrFI         297  CCAGG                CC^[ACGT]GG
SfaNI         175  GCATC                GCATC(5/9)
SmlI          443  CTCGAG               C^T[CT][GA]AG
SspBI         667  TGTACA               T^GTACA
StyI          698  CCAAGG               C^C[AT][AT]GG
TaqI          444  TCGA                 T^CGA
TthHB8I       444  TCGA                 T^CGA
XhoI          443  CTCGAG               C^TCGAG
XhoII         685  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwI          343  GATCC                GGATC(4/5)
              686  GATCC               
BsiYI         462  CCATGAGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              473  CCCATCTCCGG         
BslI          462  CCATGAGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              473  CCCATCTCCGG         
BsrI           66  ACTGG                ACTGG(1/-1)
              119  CCAGT               
MnlI          442  CCTC                 CCTC(7/6)
              446  GAGG                
NlaIV         470  GGGCCC               GG[ACGT]^[ACGT]CC
              604  GGGGCC              
Tsp45I        635  GTGAC                ^GT[GC]AC
              736  GTGAC               
TspRI          65  CACTG                CA[GC]TG[ACGT][ACGT]^
              120  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AfaI        AluI        Cfr13I      DpnII       HaeIII      
HinfI       MaeIII      MboI        MboII       MseI        
NdeII       NlaIII      RsaI        Sau3AI      Sau96I      
TfiI        Tru9I       Tsp509I     
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