Restriction map for pF1KB7221
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StyI        SwaI        
TaqI        Tru9I       Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          176  GTCTAC               GT^[AC][GT]AC
AclI          100  AACGTT               AA^CGTT
AcsI          595  AAATTT               [GA]^AATT[CT]
AfaI          369  GTAC                 GT^AC
AlwI           45  GATCC                GGATC(4/5)
AlwNI         534  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          595  AAATTT               [GA]^AATT[CT]
Asp700I       252  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI           3  GAGCAC               G[AT]GC[AT]^C
AvaII         459  GGTCC                G^G[AT]CC
BanI           70  GGCACC               G^G[CT][GA]CC
BbvI          473  GCAGC                GCAGC(8/12)
BclI          354  TGATCA               T^GATCA
BlpI          468  GCTGAGC              GC^T[ACGT]AGC
BmyI            3  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      468  GCTGAGC              GC^T[ACGT]AGC
BseRI         486  GAGGAG               GAGGAG(10/8)
BsiHKAI         3  GAGCAC               G[AT]GC[AT]^C
Bsp1286I        3  GAGCAC               G[AGT]GC[ACT]^C
BsrDI          63  CATTGC               GCAATG(2/0)
BstYI          44  AGATCC               [GA]^GATC[CT]
CelII         468  GCTGAGC              GC^T[ACGT]AGC
Cfr13I        459  GGTCC                G^G[ACGT]CC
DraII         458  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      458  GGGTCCC              [GA]G^G[ACGT]CC[CT]
FbaI          354  TGATCA               T^GATCA
Fnu4HI        473  GCAGC                GC^[ACGT]GC
ItaI          473  GCAGC                GC^[ACGT]GC
MaeII         101  ACGT                 A^CGT
MflI           44  AGATCC               [GA]^GATC[CT]
MspA1I        466  CAGCTG               C[AC]G^C[GT]G
NlaIII        414  CATG                 CATG^
PflMI         378  CCAATGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          116  GAGTC                GAGTC(4/5)
PpuMI         458  GGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I      100  AACGTT               AA^CGTT
PvuII         466  CAGCTG               CAG^CTG
RsaI          369  GTAC                 GT^AC
Sau96I        459  GGTCC                G^G[ACGT]CC
SmlI           40  CTTGAG               C^T[CT][GA]AG
SspI          311  AATATT               AAT^ATT
TfiI           21  GATTC                G^A[AT]TC
TseI          473  GCAGC                G^C[AT]GC
TspRI         534  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        378  CCAATGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII          44  AGATCC               [GA]^GATC[CT]
XmnI          252  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BbsI          391  GAAGAC               GAAGAC(2/6)
              409  GAAGAC              
BpuAI         391  GAAGAC               GAAGAC(2/6)
              409  GAAGAC              
BsiYI          75  CCTATCCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              378  CCAATGCTTGG         
BslI           75  CCTATCCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              378  CCAATGCTTGG         
BsmAI         135  GAGAC                GTCTC(1/5)
              324  GAGAC               
Cac8I          67  GCTGGC               GC[ACGT]^[ACGT]GC
              360  GCAAGC              
DpnII          45  GATC                 ^GATC
              355  GATC                
EarI          113  GAAGAG               CTCTTC(1/4)
              237  GAAGAG              
HaeIII        255  GGCC                 GG^CC
              279  GGCC                
HinfI          21  GATTC                G^A[ACGT]TC
              116  GAGTC               
HphI          105  TCACC                GGTGA(8/7)
              119  TCACC               
Ksp632I       113  GAAGAG               CTCTTC(1/4)
              237  GAAGAG              
MboI           45  GATC                 ^GATC
              355  GATC                
NdeII          45  GATC                 ^GATC
              355  GATC                
Sau3AI         45  GATC                 ^GATC
              355  GATC                
SfcI          167  CTGTAG               C^T[GA][CT]AG
              274  CTACAG              
StuI          254  AGGCCT               AGG^CCT
              278  AGGCCT              
Tsp45I        118  GTCAC                ^GT[GC]AC
              536  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BsaJI       BsmFI       BssKI       BstNI       
DdeI        EcoRII      MaeIII      MboII       MnlI        
MvaI        NlaIV       ScrFI       Tsp509I     
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