Restriction map for pF1KB7216
 (Circular) (Six-base) MAPSORT of: pF1KB7216.seq  Check: 5535  from: 1  to: 3560



pF1KB7216  3560 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:    598     598 
   Size:       3560
 
Acc65I G'GTAC_C

Cuts at:    576     576 
   Size:       3560
 
AclI AA'CG_TT

Cuts at:   3400    3400 
   Size:       3560
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1385    1817    2564    2832    1385 
   Size:        432     747     268    2113

  Fragments arranged by size: 

                2113     747     432     268
 
AfeI AGC'GCT

Cuts at:    119     119 
   Size:       3560
 
AflIII A'CryG_T

Cuts at:    912    2016    2993     912 
   Size:       1104     977    1479

  Fragments arranged by size: 

                1479    1104     977
 
AgeI A'CCGG_T

Cuts at:   1948    1948 
   Size:       3560
 
AhdI GACnn_n'nnGTC

Cuts at:    181     181 
   Size:       3560
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    184     184 
   Size:       3560
 
AlwNI CAG_nnn'CTG

Cuts at:    865    2432     865 
   Size:       1567    1993
 
ApaI G_GGCC'C

Cuts at:    314     314 
   Size:       3560
 
ApaLI G'TGCA_C

Cuts at:   2330    2330 
   Size:       3560
 
ApoI r'AATT_y

Cuts at:    564    2913    3169     564 
   Size:       2349     256     955

  Fragments arranged by size: 

                2349     955     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3560
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3560
 
AvaI C'yCGr_G

Cuts at:    286     580     286 
   Size:        294    3266
 
AvrII C'CTAG_G

Cuts at:    219     219 
   Size:       3560
 
BamHI G'GATC_C

Cuts at:    585     585 
   Size:       3560
 
BanI G'GyrC_C

Cuts at:    223     273     576     990    1238    1273     223 
   Size:         50     303     414     248      35    2510

  Fragments arranged by size: 

                2510     414     303     248      50      35
 
BanII G_rGCy'C

Cuts at:    314     371     502     574    1604     314 
   Size:         57     131      72    1030    2270

  Fragments arranged by size: 

                2270    1030     131      72      57
 
BbeI G_GCGC'C

Cuts at:    994    1242     994 
   Size:        248    3312
 
BciVI GTATCCnnnnn_n'

Cuts at:   1458    2219    3560    1458 
   Size:        761    1341    1458

  Fragments arranged by size: 

                1458    1341     761
 
BclI T'GATC_A

Cuts at:   1080    1080 
   Size:       3560
 
BglII A'GATC_T

Cuts at:   1075    3112    1075 
   Size:       2037    1523
 
BlpI GC'TnA_GC

Cuts at:    672     672 
   Size:       3560
 
Bme1580I G_kGCm'C

Cuts at:    314     397    1185    1278    2334     314 
   Size:         83     788      93    1056    1540

  Fragments arranged by size: 

                1540    1056     788      93      83
 
BmrI ACTGGGnnnn_n'

Cuts at:    772     853    1188    3332     772 
   Size:         81     335    2144    1000

  Fragments arranged by size: 

                2144    1000     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1927    1927 
   Size:       3560
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     373     178 
   Size:        195    3365
 
Bpu10I CC'TnA_GC

Cuts at:    370     370 
   Size:       3560
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    736    1615    2107    2405     736 
   Size:        879     492     298    1891

  Fragments arranged by size: 

                1891     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    272    3264     272 
   Size:       2992     568
 
BsaAI yAC'GTr

Cuts at:    913     913 
   Size:       3560
 
BsaBI GATnn'nnATC

Cuts at:      6    1074    3111       6 
   Size:       1068    2037     455

  Fragments arranged by size: 

                2037    1068     455
 
BsaHI Gr'CG_yC

Cuts at:    991    1239    1941    3437     991 
   Size:        248     702    1496    1114

  Fragments arranged by size: 

                1496    1114     702     248
 
BsaWI w'CCGG_w

Cuts at:    466     749    1270    1948    2222    2369    2780     466 
   Size:        283     521     678     274     147     411    1246

  Fragments arranged by size: 

                1246     678     521     411     283     274     147
 
BseYI C'CCAG_C

Cuts at:    985    1743    2320    2816     985 
   Size:        758     577     496    1729

  Fragments arranged by size: 

                1729     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1102    1148    2356     104 
   Size:        998      46    1208    1308

  Fragments arranged by size: 

                1308    1208     998      46
 
BsiHKAI G_wGCw'C

Cuts at:    371     574    1352    1542    2334     371 
   Size:        203     778     190     792    1597

  Fragments arranged by size: 

                1597     792     778     203     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    189     189 
   Size:       3560
 
Bsp1286I G_dGCh'C

Cuts at:    314     371     397     502     574    1185    1278    1352    1542 
   Size:         57      26     105      72     611      93      74     190

Cuts at:   1542    1604    2334     314 
   Size:         62     730    1540

  Fragments arranged by size: 

                1540     730     611     190     105      93      74      72
                  62      57      26
 
BspEI T'CCGG_A

Cuts at:   2780    2780 
   Size:       3560
 
BspHI T'CATG_A

Cuts at:   2736    2736 
   Size:       3560
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    610     824    1126    1507     610 
   Size:        214     302     381    2663

  Fragments arranged by size: 

                2663     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1852    1906    3390    3555      50 
   Size:       1802      54    1484     165      55

  Fragments arranged by size: 

                1802    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1472    1472 
   Size:       3560
 
BsrFI r'CCGG_y

Cuts at:   1558    1739    1948    1558 
   Size:        181     209    3170

  Fragments arranged by size: 

                3170     209     181
 
BssSI C'ACGA_G

Cuts at:    162    1831    2189     162 
   Size:       1669     358    1533

  Fragments arranged by size: 

                1669    1533     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    937     937 
   Size:       3560
 
BstBI TT'CG_AA

Cuts at:   1921    1921 
   Size:       3560
 
BstYI r'GATC_y

Cuts at:      1     585    1052    1075    1410    1656    2668    2754    2766 
   Size:        584     467      23     335     246    1012      86      12

Cuts at:   2766    3112       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     584     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1510    1691    1854    1510 
   Size:        181     163    3216

  Fragments arranged by size: 

                3216     181     163
 
BtsI GCAGTG_nn'

Cuts at:    150     803     150 
   Size:        653    2907
 
DraI TTT'AAA

Cuts at:    559     559 
   Size:       3560
 
DraIII CAC_nnn'GTG

Cuts at:    403     403 
   Size:       3560
 
DrdI GACnn_nn'nnGTC

Cuts at:   1266    2124    1266 
   Size:        858    2702
 
EaeI y'GGCC_r

Cuts at:   1145    1319    1710    1737    3082    1145 
   Size:        174     391      27    1345    1623

  Fragments arranged by size: 

                1623    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1145    1145 
   Size:       3560
 
EarI CTCTTCn'nnn_

Cuts at:   1583    1793    1583 
   Size:        210    3350
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1768    2078    2224    3370    1768 
   Size:        310     146    1146    1958

  Fragments arranged by size: 

                1958    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    369     572     369 
   Size:        203    3357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     373    1385    1817    2564    2832     178 
   Size:        195    1012     432     747     268     906

  Fragments arranged by size: 

                1012     906     747     432     268     195
 
EcoNI CCTnn'n_nnAGG

Cuts at:    232     232 
   Size:       3560
 
EcoO109I rG'GnC_Cy

Cuts at:    199     311     699     199 
   Size:        112     388    3060

  Fragments arranged by size: 

                3060     388     112
 
EcoRI G'AATT_C

Cuts at:    564     564 
   Size:       3560
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1579    1579 
   Size:       3560
 
FspI TGC'GCA

Cuts at:   1341    1341 
   Size:       3560
 
HaeII r_GCGC'y

Cuts at:    121     994    1242    2264    3243     121 
   Size:        873     248    1022     979     438

  Fragments arranged by size: 

                1022     979     873     438     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    167     199     371     403    1402    1432    1434    1464     167 
   Size:         32     172      32     999      30       2      30    2263

  Fragments arranged by size: 

                2263     999     172      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    599     599 
   Size:       3560
 
HindIII A'AGCT_T

Cuts at:    247     247 
   Size:       3560
 
KasI G'GCGC_C

Cuts at:    990    1238     990 
   Size:        248    3312
 
KpnI G_GTAC'C

Cuts at:    580     580 
   Size:       3560
 
MluI A'CGCG_T

Cuts at:   2993    2993 
   Size:       3560
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    223    2231    2415     223 
   Size:       2008     184    1368

  Fragments arranged by size: 

                2008    1368     184
 
MscI TGG'CCA

Cuts at:   1321    1321 
   Size:       3560
 
MslI CAynn'nnrTG

Cuts at:   1549    1676    1549 
   Size:        127    3433
 
MspA1I CmG'CkG

Cuts at:    140     477     671     985    1345    2358    2603     140 
   Size:        337     194     314     360    1013     245    1097

  Fragments arranged by size: 

                1097    1013     360     337     314     245     194
 
NaeI GCC'GGC

Cuts at:   1741    1741 
   Size:       3560
 
NarI GG'CG_CC

Cuts at:    991    1239     991 
   Size:        248    3312
 
NgoMIV G'CCGG_C

Cuts at:   1739    1739 
   Size:       3560
 
NspI r_CATG'y

Cuts at:    613    1113    2020     613 
   Size:        500     907    2153

  Fragments arranged by size: 

                2153     907     500
 
PasI CC'CwG_GG

Cuts at:    228     228 
   Size:       3560
 
PciI A'CATG_T

Cuts at:   2016    2016 
   Size:       3560
 
PmeI GTTT'AAAC

Cuts at:    559     559 
   Size:       3560
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2699    2731    2699 
   Size:         32    3528
 
PpuMI rG'GwC_Cy

Cuts at:    199     199 
   Size:       3560
 
PspOMI G'GGCC_C

Cuts at:    310     310 
   Size:       3560
 
PstI C_TGCA'G

Cuts at:    145     607    1292     145 
   Size:        462     685    2413

  Fragments arranged by size: 

                2413     685     462
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3560
 
PvuII CAG'CTG

Cuts at:    477     985    1345     477 
   Size:        508     360    2692

  Fragments arranged by size: 

                2692     508     360
 
RsrII CG'GwC_CG

Cuts at:   1755    1755 
   Size:       3560
 
SacI G_AGCT'C

Cuts at:    371     574     371 
   Size:        203    3357
 
SalI G'TCGA_C

Cuts at:    597     597 
   Size:       3560
 
SanDI GG'GwC_CC

Cuts at:    199     199 
   Size:       3560
 
SapI GCTCTTCn'nnn_

Cuts at:   1583    1793    1583 
   Size:        210    3350
 
SbfI CC_TGCA'GG

Cuts at:    607     607 
   Size:       3560
 
ScaI AGT'ACT

Cuts at:   3077    3077 
   Size:       3560
 
SfcI C'TryA_G

Cuts at:     33     141     603    1288    2281    2472      33 
   Size:        108     462     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     462     191     108
 
SfoI GGC'GCC

Cuts at:    992    1240     992 
   Size:        248    3312
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3560
 
SmaI CCC'GGG

Cuts at:    582     582 
   Size:       3560
 
SmlI C'TyrA_G

Cuts at:    715    1630    2122    2384     715 
   Size:        915     492     262    1891

  Fragments arranged by size: 

                1891     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3560
 
SphI G_CATG'C

Cuts at:    613    1113     613 
   Size:        500    3060
 
StyI C'CwwG_G

Cuts at:    219     694    1048     219 
   Size:        475     354    2731

  Fragments arranged by size: 

                2731     475     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    316     338    1143    1276    1735    1944     316 
   Size:         22     805     133     459     209    1932

  Fragments arranged by size: 

                1932     805     459     209     133      22
 
TatI w'GTAC_w

Cuts at:   3075    3075 
   Size:       3560
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3077    3109    3077 
   Size:         32    3528
 
Tth111I GACn'n_nGTC

Cuts at:   1357    1357 
   Size:       3560
 
XbaI T'CTAG_A

Cuts at:    591     591 
   Size:       3560
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1055    1055 
   Size:       3560
 
XmaI C'CCGG_G

Cuts at:    580     580 
   Size:       3560
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI     PciI     PmeI     PpiI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AleI     AloI     AscI     BaeI     BbsI
    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI    BsaXI    BseRI
     BsgI    BsiWI     BsmI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmlI    PshAI     PsiI    PspXI     PsrI    SacII
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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