Restriction map for pF1KB7215
##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AfaI          115  GTAC                 GT^AC
AlwI          133  GATCC                GGATC(4/5)
BsaJI          50  CCCTGG               C^C[ACGT][ACGT]GG
BsgI          190  CTGCAC               GTGCAG(16/14)
BsiWI         114  CGTACG               C^GTACG
BsmAI          33  GTCTC                GTCTC(1/5)
BspMI         188  ACCTGC               ACCTGC(4/8)
DpnII         133  GATC                 ^GATC
HinfI         213  GATTC                G^A[ACGT]TC
MaeII         113  ACGT                 A^CGT
MboI          133  GATC                 ^GATC
MseI           71  TTAA                 T^TAA
MspA1I        104  CAGCTG               C[AC]G^C[GT]G
NdeII         133  GATC                 ^GATC
NlaIII         27  CATG                 CATG^
PvuII         104  CAGCTG               CAG^CTG
RsaI          115  GTAC                 GT^AC
Sau3AI        133  GATC                 ^GATC
SexAI         316  ACCAGGT              A^CC[AT]GGT
TfiI          213  GATTC                G^A[AT]TC
Tru9I          71  TTAA                 T^TAA
XcmI            8  CCAGCAGCTTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AluI           13  AGCT                 AG^CT
              105  AGCT                
AvaII           5  GGTCC                G^G[AT]CC
               21  GGTCC               
BbvI           11  GCAGC                GCAGC(8/12)
              119  GCTGC               
BseRI         306  CTCCTC               GAGGAG(10/8)
              309  CTCCTC              
BsiYI         274  CCTGAGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              311  CCTCTACCAGG         
BslI          274  CCTGAGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              311  CCTCTACCAGG         
BsrI          228  ACTGG                ACTGG(1/-1)
              141  CCAGT               
BstF5I        249  GGATG                GGATG(2/0)
              283  GGATG               
Cfr13I          5  GGTCC                G^G[ACGT]CC
               21  GGTCC               
DdeI          275  CTGAG                C^T[ACGT]AG
              326  CTCAG               
EcoNI         274  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              311  CCTCTACCAGG         
Fnu4HI         11  GCAGC                GC^[ACGT]GC
              119  GCTGC               
FokI          249  GGATG                GGATG(9/13)
              283  GGATG               
HaeIII        266  GGCC                 GG^CC
              291  GGCC                
ItaI           11  GCAGC                GC^[ACGT]GC
              119  GCTGC               
MboII         232  GAAGA                GAAGA(8/7)
              253  GAAGA               
NlaIV           4  GGGTCC               GG[ACGT]^[ACGT]CC
              304  GGCTCC              
Sau96I          5  GGTCC                G^G[ACGT]CC
               21  GGTCC               
TseI           11  GCAGC                G^C[AT]GC
              119  GCTGC               
Tsp45I         46  GTGAC                ^GT[GC]AC
              146  GTCAC               
TspRI         142  CAGTG                CA[GC]TG[ACGT][ACGT]^
              193  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BssKI       BstNI       EcoRII      MaeIII      MnlI        
MvaI        ScrFI       
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