Restriction map for pF1KB7196
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AfaI        AflII       AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      CpoI        DraI        DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       EcoT22I     
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshBI       
PvuI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AclI          554  AACGTT               AA^CGTT
AflIII        984  ACGTGT               A^C[GA][CT]GT
Alw44I       1054  GTGCAC               G^TGCAC
AlwNI        1043  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1054  GTGCAC               G^TGCAC
Asp700I       552  GAAACGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI          803  TGATCA               T^GATCA
BpmI           57  CTGGAG               CTGGAG(16/14)
BsaBI         762  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1001  ACCGGA               [AT]^CCGG[AT]
BspDI         759  ATCGAT               AT^CGAT
BspHI         383  TCATGA               T^CATGA
BssSI        1057  CACGAG               CACGAG(-5/-1)
ClaI          759  ATCGAT               AT^CGAT
DraIII        485  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         453  CCTTGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         841  GAATTC               G^AATTC
FbaI          803  TGATCA               T^GATCA
HgaI          387  GACGC                GACGC(5/10)
MamI          762  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI         109  CCAGTGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1394  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1222  GACCCTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      554  AACGTT               AA^CGTT
PstI          869  CTGCAG               CTGCA^G
RcaI          383  TCATGA               T^CATGA
SacI           66  GAGCTC               GAGCT^C
SfaNI        1045  GATGC                GCATC(5/9)
SmaI          678  CCCGGG               CCC^GGG
SphI         1013  GCATGC               GCATG^C
Van91I        109  CCAGTGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          497  CCACTTTCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         678  CCCGGG               C^CCGGG
XmaI          678  CCCGGG               C^CCGGG
XmnI          552  GAAACGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI          678  CCCGGG               C^[CT]CG[GA]G
              779  CTCGGG              
BamHI           3  GGATCC               G^GATCC
              635  GGATCC              
BanII          66  GAGCTC               G[GA]GC[CT]^C
             1005  GAGCCC              
BcnI          678  CCCGG                CC^[GC]GG
              679  CCGGG               
BsiYI         109  CCAGTGATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              453  CCTTGTGGAGG         
BslI          109  CCAGTGATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              453  CCTTGTGGAGG         
BsoBI         678  CCCGGG               C^[CT]CG[GA]G
              779  CTCGGG              
BsrDI         344  GCAATG               GCAATG(2/0)
              524  GCAATG              
BsrI          303  ACTGG                ACTGG(1/-1)
              109  CCAGT               
DdeI          916  CTGAG                C^T[ACGT]AG
             1284  CTCAG               
DraII        1313  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1394  AGGACCT             
EcoO109I     1313  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1394  AGGACCT             
EcoT14I       476  CCAAGG               C^C[AT][AT]GG
             1145  CCAAGG              
HinfI         332  GAATC                G^A[ACGT]TC
              757  GAATC               
HphI          246  GGTGA                GGTGA(8/7)
              484  TCACC               
NciI          678  CCCGG                CC^[GC]GG
              679  CCGGG               
NspI         1013  GCATGC               [GA]CATG^[CT]
             1031  ACATGC              
PvuII         872  CAGCTG               CAG^CTG
             1450  CAGCTG              
SfcI          127  CTACAG               C^T[GA][CT]AG
              869  CTGCAG              
StyI          476  CCAAGG               C^C[AT][AT]GG
             1145  CCAAGG              
TfiI          332  GAATC                G^A[AT]TC
              757  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AspHI       AvaII       BbvI        BmyI        BsaJI       
BseRI       BsiHKAI     BsmAI       BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DpnII       EarI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com