Restriction map for pF1KB7195
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MroI        MseI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflIII        202  ACATGT               A^C[GA][CT]GT
AlwI          159  GGATC                GGATC(4/5)
AlwNI         357  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          352  GGGCCC               GGGCC^C
AspHI         251  GTGCTC               G[AT]GC[AT]^C
AvaII         174  GGACC                G^G[AT]CC
BalI          287  TGGCCA               TGG^CCA
BanII         352  GGGCCC               G[GA]GC[CT]^C
BbsI            3  GAAGAC               GAAGAC(2/6)
BbvI           50  GCTGC                GCAGC(8/12)
BcnI          244  CCCGG                CC^[GC]GG
BpmI           10  CTCCAG               CTGGAG(16/14)
BpuAI           3  GAAGAC               GAAGAC(2/6)
BsiHKAI       251  GTGCTC               G[AT]GC[AT]^C
BspLU11I      202  ACATGT               A^CATGT
BsrDI          81  CATTGC               GCAATG(2/0)
BstXI         155  CCAGGGATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         159  GGATCT               [GA]^GATC[CT]
Cac8I         139  GCTGGC               GC[ACGT]^[ACGT]GC
DpnII         160  GATC                 ^GATC
EaeI          287  TGGCCA               [CT]^GGCC[GA]
Fnu4HI         50  GCTGC                GC^[ACGT]GC
HapII         245  CCGG                 C^CGG
HinfI         265  GATTC                G^A[ACGT]TC
HpaII         245  CCGG                 C^CGG
ItaI           50  GCTGC                GC^[ACGT]GC
MboI          160  GATC                 ^GATC
MflI          159  GGATCT               [GA]^GATC[CT]
MluNI         287  TGGCCA               TGG^CCA
MnlI          344  GAGG                 CCTC(7/6)
MscI          287  TGGCCA               TGG^CCA
MspI          245  CCGG                 C^CGG
NciI          244  CCCGG                CC^[GC]GG
NdeII         160  GATC                 ^GATC
NspI          202  ACATGT               [GA]CATG^[CT]
PpuMI         173  AGGACCC              [GA]G^G[AT]CC[CT]
Sau3AI        160  GATC                 ^GATC
SfaNI         119  GCATC                GCATC(5/9)
StuI          345  AGGCCT               AGG^CCT
TfiI          265  GATTC                G^A[AT]TC
TseI           50  GCTGC                G^C[AT]GC
XcmI          294  CCAGTGTCACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         159  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AccI          223  GTATAC               GT^[AC][GT]AC
              273  GTATAC              
AfaI          206  GTAC                 GT^AC
              335  GTAC                
BsaJI         155  CCAGGG               C^C[ACGT][ACGT]GG
              348  CCTGGG              
Bst1107I      223  GTATAC               GTA^TAC
              273  GTATAC              
BstZ17I       223  GTATAC               GTA^TAC
              273  GTATAC              
DraII         173  AGGACCC              [GA]G^G[ACGT]CC[CT]
              351  GGGGCCC             
DraIII        129  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              291  CACCCAGTG           
EcoO109I      173  AGGACCC              [GA]G^G[ACGT]CC[CT]
              351  GGGGCCC             
MaeIII        299  GTCAC                ^GT[ACGT]AC
              314  GTGAC               
MwoI           50  GCTGCAATAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              253  GCTCACCATGC         
NlaIII        203  CATG                 CATG^
              259  CATG                
RsaI          206  GTAC                 GT^AC
              335  GTAC                
Tsp45I        299  GTCAC                ^GT[GC]AC
              314  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AluI        BmyI        Bsp1286I    BsrI        BssKI       
BstNI       Cfr13I      EcoRII      HaeIII      HphI        
MboII       MvaI        NlaIV       Sau96I      ScrFI       
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com