Restriction map for pF1KB7194
 (Linear) (Six-base) MAPSORT of: KIBB7194.seq  Check: 1949  from: 1  to: 2028



KIBB7194.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     648    2028 
   Size:        648    1380
 
AatII G_ACGT'C

Cuts at:      0    1622    2028 
   Size:       1622     406
 
AccI GT'mk_AC

Cuts at:      0     329     722    1373    1943    2028 
   Size:        329     393     651     570      85

  Fragments arranged by size: 

                 651     570     393     329      85
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     399    1078    2028 
   Size:        399     679     950

  Fragments arranged by size: 

                 950     679     399
 
AfeI AGC'GCT

Cuts at:      0      40    2028 
   Size:         40    1988
 
AflIII A'CryG_T

Cuts at:      0     717    2028 
   Size:        717    1311
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     680    1155    1990    2028 
   Size:        680     475     835      38

  Fragments arranged by size: 

                 835     680     475      38
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1147    1179    2028 
   Size:       1147      32     849

  Fragments arranged by size: 

                1147     849      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     705     783    1815    2028 
   Size:        705      78    1032     213

  Fragments arranged by size: 

                1032     705     213      78
 
ApaI G_GGCC'C

Cuts at:      0    1908    2014    2028 
   Size:       1908     106      14

  Fragments arranged by size: 

                1908     106      14
 
ApoI r'AATT_y

Cuts at:      0    1582    2028 
   Size:       1582     446
 
AvaI C'yCGr_G

Cuts at:      0     340    1044    2028 
   Size:        340     704     984

  Fragments arranged by size: 

                 984     704     340
 
BanI G'GyrC_C

Cuts at:      0      64     169     736     954    1765    2028 
   Size:         64     105     567     218     811     263

  Fragments arranged by size: 

                 811     567     263     218     105      64
 
BanII G_rGCy'C

Cuts at:      0     649     757    1405    1908    1955    2014    2028 
   Size:        649     108     648     503      47      59      14

  Fragments arranged by size: 

                 649     648     503     108      59      47      14
 
BbeI G_GCGC'C

Cuts at:      0      68     173    1769    2028 
   Size:         68     105    1596     259

  Fragments arranged by size: 

                1596     259     105      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     378     756     982    1793    2028 
   Size:        378     378     226     811     235

  Fragments arranged by size: 

                 811     378     378     235     226
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424     458    1743    1777    2028 
   Size:        424      34    1285      34     251

  Fragments arranged by size: 

                1285     424     251      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      62     137     451     673     734    2028 
   Size:         62      75     314     222      61    1294

  Fragments arranged by size: 

                1294     314     222      75      62      61
 
BglII A'GATC_T

Cuts at:      0    1178    2028 
   Size:       1178     850
 
Bme1580I G_kGCm'C

Cuts at:      0     103     959    1908    2003    2014    2028 
   Size:        103     856     949      95      11      14

  Fragments arranged by size: 

                 949     856     103      95      14      11
 
BmgBI CAC'GTC

Cuts at:      0     315     671     720     965    2028 
   Size:        315     356      49     245    1063

  Fragments arranged by size: 

                1063     356     315     245      49
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     214    2028 
   Size:        214    1814
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1226    1650    2028 
   Size:       1226     424     378

  Fragments arranged by size: 

                1226     424     378
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     762    1319    2028 
   Size:        133     629     557     709

  Fragments arranged by size: 

                 709     629     557     133
 
Bpu10I CC'TnA_GC

Cuts at:      0    1949    2028 
   Size:       1949      79
 
BsaAI yAC'GTr

Cuts at:      0     513     552    1331    2028 
   Size:        513      39     779     697

  Fragments arranged by size: 

                 779     697     513      39
 
BsaBI GATnn'nnATC

Cuts at:      0     478    1183    2028 
   Size:        478     705     845

  Fragments arranged by size: 

                 845     705     478
 
BsaHI Gr'CG_yC

Cuts at:      0      65     170    1619    1766    2028 
   Size:         65     105    1449     147     262

  Fragments arranged by size: 

                1449     262     147     105      65
 
BsaWI w'CCGG_w

Cuts at:      0    1187    2028 
   Size:       1187     841
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1147    1177    2028 
   Size:       1147      30     851

  Fragments arranged by size: 

                1147     851      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       3     816    1155    1695    2028 
   Size:          3     813     339     540     333

  Fragments arranged by size: 

                 813     540     339     333       3
 
BseYI C'CCAG_C

Cuts at:      0      41    1026    1222    1916    1963    2028 
   Size:         41     985     196     694      47      65

  Fragments arranged by size: 

                 985     694     196      65      47      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    2028 
   Size:       1084     944
 
BsiEI CG_ry'CG

Cuts at:      0     203     247     726    1763    2028 
   Size:        203      44     479    1037     265

  Fragments arranged by size: 

                1037     479     265     203      44
 
BsiHKAI G_wGCw'C

Cuts at:      0    1955    2028 
   Size:       1955      73
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     276    1731    2028 
   Size:        276    1455     297

  Fragments arranged by size: 

                1455     297     276
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     649     757     959    1405    1908    1955    2003 
   Size:        103     546     108     202     446     503      47      48

Cuts at:   2003    2014    2028 
   Size:         11      14

  Fragments arranged by size: 

                 546     503     446     202     108     103      48      47
                  14      11
 
BspEI T'CCGG_A

Cuts at:      0    1187    2028 
   Size:       1187     841
 
BspHI T'CATG_A

Cuts at:      0    1436    2028 
   Size:       1436     592
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     648    2028 
   Size:        648    1380
 
BsrBI CCG'CTC

Cuts at:      0    1350    2028 
   Size:       1350     678
 
BsrFI r'CCGG_y

Cuts at:      0     249     352     532    1094    2028 
   Size:        249     103     180     562     934

  Fragments arranged by size: 

                 934     562     249     180     103
 
BsrGI T'GTAC_A

Cuts at:      0     797    2028 
   Size:        797    1231
 
BssHII G'CGCG_C

Cuts at:      0     143     304    2028 
   Size:        143     161    1724

  Fragments arranged by size: 

                1724     161     143
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1699    2028 
   Size:       1699     329
 
BstYI r'GATC_y

Cuts at:      0     701    1100    1178    2028 
   Size:        701     399      78     850

  Fragments arranged by size: 

                 850     701     399      78
 
Bsu36I CC'TnA_GG

Cuts at:      0     706    2028 
   Size:        706    1322
 
BtgI C'CryG_G

Cuts at:      0       5     241     455     626     758    2028 
   Size:          5     236     214     171     132    1270

  Fragments arranged by size: 

                1270     236     214     171     132       5
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     144     966    2028 
   Size:        144     822    1062

  Fragments arranged by size: 

                1062     822     144
 
BtsI GCAGTG_nn'

Cuts at:      0    1408    1935    2028 
   Size:       1408     527      93

  Fragments arranged by size: 

                1408     527      93
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     268    2028 
   Size:        268    1760
 
EaeI y'GGCC_r

Cuts at:      0       2      89     200     244     452     674    1571    2028 
   Size:          2      87     111      44     208     222     897     457

  Fragments arranged by size: 

                 897     457     222     208     111      87      44       2
            
 
EagI C'GGCC_G

Cuts at:      0     200     244    2028 
   Size:        200      44    1784

  Fragments arranged by size: 

                1784     200      44
 
EarI CTCTTCn'nnn_

Cuts at:      0     472    1340    1393    1960    2028 
   Size:        472     868      53     567      68

  Fragments arranged by size: 

                 868     567     472      68      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     136     508     519    2028 
   Size:        136     372      11    1509

  Fragments arranged by size: 

                1509     372     136      11
 
EcoICRI GAG'CTC

Cuts at:      0    1953    2028 
   Size:       1953      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     399     762    1078    1319    2028 
   Size:        133     266     363     316     241     709

  Fragments arranged by size: 

                 709     363     316     266     241     133
 
EcoO109I rG'GnC_Cy

Cuts at:      0     178    1194    1461    1904    2010    2011    2028 
   Size:        178    1016     267     443     106       1      17

  Fragments arranged by size: 

                1016     443     267     178     106      17       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120    2028 
   Size:        120    1908
 
FspI TGC'GCA

Cuts at:      0    1663    2028 
   Size:       1663     365
 
FspAI rTGC'GCAy

Cuts at:      0    1663    2028 
   Size:       1663     365
 
HaeII r_GCGC'y

Cuts at:      0      42      68     173    1769    2028 
   Size:         42      26     105    1596     259

  Fragments arranged by size: 

                1596     259     105      42      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1351    1383    1728    1760    2008    2028 
   Size:       1351      32     345      32     248      20

  Fragments arranged by size: 

                1351     345     248      32      32      20
 
HincII GTy'rAC

Cuts at:      0     723    1374    1944    2028 
   Size:        723     651     570      84

  Fragments arranged by size: 

                 723     651     570      84
 
KasI G'GCGC_C

Cuts at:      0      64     169    1765    2028 
   Size:         64     105    1596     263

  Fragments arranged by size: 

                1596     263     105      64
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    2028 
   Size:        254    1774
 
MscI TGG'CCA

Cuts at:      0      91     454    1573    2028 
   Size:         91     363    1119     455

  Fragments arranged by size: 

                1119     455     363      91
 
MslI CAynn'nnrTG

Cuts at:      0    1137    1831    2028 
   Size:       1137     694     197

  Fragments arranged by size: 

                1137     694     197
 
MspA1I CmG'CkG

Cuts at:      0       7     243    1030    1515    1803    1845    1880    1992 
   Size:          7     236     787     485     288      42      35     112

Cuts at:   1992    2028 
   Size:         36

  Fragments arranged by size: 

                 787     485     288     236     112      42      36      35
                   7
 
NaeI GCC'GGC

Cuts at:      0     534    2028 
   Size:        534    1494
 
NarI GG'CG_CC

Cuts at:      0      65     170    1766    2028 
   Size:         65     105    1596     262

  Fragments arranged by size: 

                1596     262     105      65
 
NcoI C'CATG_G

Cuts at:      0     626    2028 
   Size:        626    1402
 
NgoMIV G'CCGG_C

Cuts at:      0     532    2028 
   Size:        532    1496
 
NotI GC'GGCC_GC

Cuts at:      0     244    2028 
   Size:        244    1784
 
NspI r_CATG'y

Cuts at:      0    1428    1560    1836    2028 
   Size:       1428     132     276     192

  Fragments arranged by size: 

                1428     276     192     132
 
PasI CC'CwG_GG

Cuts at:      0      23    2028 
   Size:         23    2005
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     356    1815    2028 
   Size:        356    1459     213

  Fragments arranged by size: 

                1459     356     213
 
PfoI T'CCnGG_A

Cuts at:      0    1296    2028 
   Size:       1296     732
 
PmlI CAC'GTG

Cuts at:      0    1331    2028 
   Size:       1331     697
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1147    1179    2028 
   Size:       1147      32     849

  Fragments arranged by size: 

                1147     849      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     178    1461    2028 
   Size:        178    1283     567

  Fragments arranged by size: 

                1283     567     178
 
PshAI GACnn'nnGTC

Cuts at:      0     967    2028 
   Size:        967    1061
 
PspOMI G'GGCC_C

Cuts at:      0    1904    2010    2028 
   Size:       1904     106      18

  Fragments arranged by size: 

                1904     106      18
 
PspXI vC'TCGA_Gb

Cuts at:      0     340    2028 
   Size:        340    1688
 
PstI C_TGCA'G

Cuts at:      0     701    1679    2028 
   Size:        701     978     349

  Fragments arranged by size: 

                 978     701     349
 
PvuI CG_AT'CG

Cuts at:      0    1763    2028 
   Size:       1763     265
 
PvuII CAG'CTG

Cuts at:      0    1515    1803    1845    1992    2028 
   Size:       1515     288      42     147      36

  Fragments arranged by size: 

                1515     288     147      42      36
 
SacI G_AGCT'C

Cuts at:      0    1955    2028 
   Size:       1955      73
 
SacII CC_GC'GG

Cuts at:      0       8     244    2028 
   Size:          8     236    1784

  Fragments arranged by size: 

                1784     236       8
 
SalI G'TCGA_C

Cuts at:      0     721    1372    1942    2028 
   Size:        721     651     570      86

  Fragments arranged by size: 

                 721     651     570      86
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1340    1393    1960    2028 
   Size:       1340      53     567      68

  Fragments arranged by size: 

                1340     567      68      53
 
ScaI AGT'ACT

Cuts at:      0    1564    1633    2028 
   Size:       1564      69     395

  Fragments arranged by size: 

                1564     395      69
 
SfcI C'TryA_G

Cuts at:      0     279     697    1675    2028 
   Size:        279     418     978     353

  Fragments arranged by size: 

                 978     418     353     279
 
SfoI GGC'GCC

Cuts at:      0      66     171    1767    2028 
   Size:         66     105    1596     261

  Fragments arranged by size: 

                1596     261     105      66
 
SgrAI Cr'CCGG_yG

Cuts at:      0     249    2028 
   Size:        249    1779
 
SmlI C'TyrA_G

Cuts at:      0     340    2028 
   Size:        340    1688
 
SphI G_CATG'C

Cuts at:      0    1428    1560    2028 
   Size:       1428     132     468

  Fragments arranged by size: 

                1428     468     132
 
StyI C'CwwG_G

Cuts at:      0     234     626     650    2028 
   Size:        234     392      24    1378

  Fragments arranged by size: 

                1378     392     234      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     325    1236    1509    1516    1930    2028 
   Size:        325     911     273       7     414      98

  Fragments arranged by size: 

                 911     414     325     273      98       7
 
TatI w'GTAC_w

Cuts at:      0     797    1562    1631    2028 
   Size:        797     765      69     397

  Fragments arranged by size: 

                 797     765     397      69
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1242    2028 
   Size:       1242     786
 
XhoI C'TCGA_G

Cuts at:      0     340    2028 
   Size:        340    1688
 
XmnI GAAnn'nnTTC

Cuts at:      0     364    1087    1582    2028 
   Size:        364     723     495     446

  Fragments arranged by size: 

                 723     495     446     364
 
ZraI GAC'GTC

Cuts at:      0    1620    2028 
   Size:       1620     408
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AlfI     AloI
    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
     BcgI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PasI    PflMI     PfoI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII     SacI
    SacII     SalI     SapI     ScaI     SfcI     SfoI    SgrAI     SmlI
     SphI     StyI    TaqII     TatI     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AleI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI
    BfrBI     BlpI     BmtI    BpuEI     BsaI    BsiWI     BsmI    BsrDI
    BssSI   BstAPI    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI
   DraIII    EcoNI    EcoRI    EcoRV     FseI  HindIII     HpaI     KpnI
     MfeI     MluI     NdeI     NheI     NruI     NsiI     PacI     PciI
     PmeI     PsiI     PsrI    RsrII    SanDI     SbfI    SexAI     SfiI
     SgfI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com