Restriction map for pF1KB7173
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     CfoI        Cfr10I      ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AhdI            7  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI           7  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1568  TGGCCA               TGG^CCA
BamHI         515  GGATCC               G^GATCC
BbsI         1411  GAAGAC               GAAGAC(2/6)
BciVI         699  GTATCC               GTATCC(6/5)
BclI           95  TGATCA               T^GATCA
BcnI          750  CCCGG                CC^[GC]GG
BfaI         1027  CTAG                 C^TAG
BpuAI        1411  GAAGAC               GAAGAC(2/6)
BsgI          817  CTGCAC               GTGCAG(16/14)
BspHI         338  TCATGA               T^CATGA
BsrBI         473  GAGCGG               CCGCTC(-3/-3)
BsrDI         323  GCAATG               GCAATG(2/0)
BstEII        150  GGTGACC              G^GT[ACGT]ACC
BstPI         150  GGTGACC              G^GT[ACGT]ACC
DraI          826  TTTAAA               TTT^AAA
DraIII       1114  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I        7  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       150  GGTGACC              G^GT[ACGT]ACC
FbaI           95  TGATCA               T^GATCA
MaeI         1027  CTAG                 C^TAG
MluNI        1568  TGGCCA               TGG^CCA
MscI         1568  TGGCCA               TGG^CCA
NciI          750  CCCGG                CC^[GC]GG
PflMI         498  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         479  AGGACCC              [GA]G^G[AT]CC[CT]
PstI           10  CTGCAG               CTGCA^G
PvuII          91  CAGCTG               CAG^CTG
RcaI          338  TCATGA               T^CATGA
SacI          307  GAGCTC               GAGCT^C
ScaI          893  AGTACT               AGT^ACT
SexAI         131  ACCTGGT              A^CC[AT]GGT
SfcI           10  CTGCAG               C^T[GA][CT]AG
SmlI          979  CTCAAG               C^T[CT][GA]AG
TaqI         1328  TCGA                 T^CGA
TfiI          769  GAATC                G^A[AT]TC
TthHB8I      1328  TCGA                 T^CGA
Van91I        498  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          567  GAATTT               [GA]^AATT[CT]
             1148  AAATTT              
AfaI          446  GTAC                 GT^AC
              894  GTAC                
AlwNI         235  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              499  CAGCTCCTG           
ApaI          351  GGGCCC               GGGCC^C
              595  GGGCCC              
ApoI          567  GAATTT               [GA]^AATT[CT]
             1148  AAATTT              
AspHI         307  GAGCTC               G[AT]GC[AT]^C
              559  GTGCTC              
BsaWI        1049  ACCGGA               [AT]^CCGG[AT]
             1499  ACCGGA              
BseRI         438  CTCCTC               GAGGAG(10/8)
              614  CTCCTC              
BsiHKAI       307  GAGCTC               G[AT]GC[AT]^C
              559  GTGCTC              
BspMI           8  ACCTGC               ACCTGC(4/8)
              282  GCAGGT              
BstYI         515  GGATCC               [GA]^GATC[CT]
              951  GGATCT              
EarI          463  CTCTTC               CTCTTC(1/4)
             1453  GAAGAG              
EcoNI         417  CCTGCCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              462  CCTCTTCCAGG         
Ksp632I       463  CTCTTC               CTCTTC(1/4)
             1453  GAAGAG              
MflI          515  GGATCC               [GA]^GATC[CT]
              951  GGATCT              
MspA1I         91  CAGCTG               C[AC]G^C[GT]G
             1254  CAGCGG              
NcoI           29  CCATGG               C^CATGG
             1460  CCATGG              
NspI          278  ACATGC               [GA]CATG^[CT]
             1541  ACATGC              
RsaI          446  GTAC                 GT^AC
              894  GTAC                
StuI          107  AGGCCT               AGG^CCT
             1368  AGGCCT              
Tsp509I       568  AATT                 ^AATT
             1149  AATT                
XhoII         515  GGATCC               [GA]^GATC[CT]
              951  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AluI        AlwI        AocI        AvaI        
AvaII       BanI        BanII       BbvI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Bsu36I      Cac8I       CelII       Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eco81I      
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
Tru9I       TseI        Tsp45I      TspRI       
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