Restriction map for pF1KB7173
 (Linear) (Six-base) MAPSORT of: KIBB7173.seq  Check: 2823  from: 1  to: 1590



KIBB7173.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     273    1590 
   Size:        273    1317
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     883    1408    1590 
   Size:        232     651     525     182

  Fragments arranged by size: 

                 651     525     232     182
 
AhdI GACnn_n'nnGTC

Cuts at:      0      12    1590 
   Size:         12    1578
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     106     138    1590 
   Size:        106      32    1452

  Fragments arranged by size: 

                1452     106      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     240     504    1590 
   Size:        240     264    1086

  Fragments arranged by size: 

                1086     264     240
 
ApaI G_GGCC'C

Cuts at:      0     355     599    1590 
   Size:        355     244     991

  Fragments arranged by size: 

                 991     355     244
 
ApoI r'AATT_y

Cuts at:      0     567    1148    1590 
   Size:        567     581     442

  Fragments arranged by size: 

                 581     567     442
 
AvaI C'yCGr_G

Cuts at:      0     247     484     592    1590 
   Size:        247     237     108     998

  Fragments arranged by size: 

                 998     247     237     108
 
BamHI G'GATC_C

Cuts at:      0     515    1590 
   Size:        515    1075
 
BanI G'GyrC_C

Cuts at:      0     693     753     832    1590 
   Size:        693      60      79     758

  Fragments arranged by size: 

                 758     693      79      60
 
BanII G_rGCy'C

Cuts at:      0     259     311     355     539     599    1478    1590 
   Size:        259      52      44     184      60     879     112

  Fragments arranged by size: 

                 879     259     184     112      60      52      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1418    1590 
   Size:       1418     172
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     710    1590 
   Size:        710     880
 
BclI T'GATC_A

Cuts at:      0      95    1590 
   Size:         95    1495
 
BlpI GC'TnA_GC

Cuts at:      0      89     904    1234    1590 
   Size:         89     815     330     356

  Fragments arranged by size: 

                 815     356     330      89
 
Bme1580I G_kGCm'C

Cuts at:      0     355     589     599     638     974    1590 
   Size:        355     234      10      39     336     616

  Fragments arranged by size: 

                 616     355     336     234      39      10
 
BmgBI CAC'GTC

Cuts at:      0     387    1169    1590 
   Size:        387     782     421

  Fragments arranged by size: 

                 782     421     387
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     253    1130    1590 
   Size:        253     877     460

  Fragments arranged by size: 

                 877     460     253
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     312    1590 
   Size:        312    1278
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     397     426     547     768     865    1590 
   Size:         94     303      29     121     221      97     725

  Fragments arranged by size: 

                 725     303     221     121      97      94      29
 
Bpu10I CC'TnA_GC

Cuts at:      0    1107    1590 
   Size:       1107     483
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     964    1590 
   Size:        964     626
 
BsaWI w'CCGG_w

Cuts at:      0    1049    1499    1590 
   Size:       1049     450      91

  Fragments arranged by size: 

                1049     450      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136     388     418    1529    1559    1590 
   Size:        106      30     252      30    1111      30      31

  Fragments arranged by size: 

                1111     252     106      31      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     429     605    1590 
   Size:        429     176     985

  Fragments arranged by size: 

                 985     429     176
 
BseYI C'CCAG_C

Cuts at:      0     491     497     520    1182    1252    1590 
   Size:        491       6      23     662      70     338

  Fragments arranged by size: 

                 662     491     338      70      23       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    1590 
   Size:        802     788
 
BsiHKAI G_wGCw'C

Cuts at:      0     311     563    1590 
   Size:        311     252    1027

  Fragments arranged by size: 

                1027     311     252
 
Bsp1286I G_dGCh'C

Cuts at:      0     259     311     355     539     563     589     599     638 
   Size:        259      52      44     184      24      26      10      39

Cuts at:    638     974    1478    1590 
   Size:        336     504     112

  Fragments arranged by size: 

                 504     336     259     184     112      52      44      39
                  26      24      10
 
BspHI T'CATG_A

Cuts at:      0     338    1590 
   Size:        338    1252
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      17     273    1590 
   Size:         17     256    1317

  Fragments arranged by size: 

                1317     256      17
 
BsrBI CCG'CTC

Cuts at:      0     475    1590 
   Size:        475    1115
 
BsrDI GCAATG_nn'

Cuts at:      0     330    1590 
   Size:        330    1260
 
BstEII G'GTnAC_C

Cuts at:      0     150    1590 
   Size:        150    1440
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     132     690    1143    1590 
   Size:        132     558     453     447

  Fragments arranged by size: 

                 558     453     447     132
 
BstYI r'GATC_y

Cuts at:      0     515     951    1590 
   Size:        515     436     639

  Fragments arranged by size: 

                 639     515     436
 
Bsu36I CC'TnA_GG

Cuts at:      0     511     887    1480    1590 
   Size:        511     376     593     110

  Fragments arranged by size: 

                 593     511     376     110
 
BtgI C'CryG_G

Cuts at:      0      29     368    1460    1590 
   Size:         29     339    1092     130

  Fragments arranged by size: 

                1092     339     130      29
 
BtsI GCAGTG_nn'

Cuts at:      0    1218    1590 
   Size:       1218     372
 
DraI TTT'AAA

Cuts at:      0     828    1590 
   Size:        828     762
 
DraIII CAC_nnn'GTG

Cuts at:      0    1119    1590 
   Size:       1119     471
 
EaeI y'GGCC_r

Cuts at:      0     221    1307    1568    1590 
   Size:        221    1086     261      22

  Fragments arranged by size: 

                1086     261     221      22
 
EarI CTCTTCn'nnn_

Cuts at:      0     469    1448    1590 
   Size:        469     979     142

  Fragments arranged by size: 

                 979     469     142
 
EcoICRI GAG'CTC

Cuts at:      0     309    1590 
   Size:        309    1281
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     232     397     426     547     768     865     883 
   Size:         94     138     165      29     121     221      97      18

Cuts at:    883    1408    1590 
   Size:        525     182

  Fragments arranged by size: 

                 525     221     182     165     138     121      97      94
                  29      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     421     466    1590 
   Size:        421      45    1124

  Fragments arranged by size: 

                1124     421      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144     351     352     429     480     596     945    1179 
   Size:        144     207       1      77      51     116     349     234

Cuts at:   1179    1590 
   Size:        411

  Fragments arranged by size: 

                 411     349     234     207     144     116      77      51
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     912    1590 
   Size:        912     678
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1182    1214    1590 
   Size:         30    1152      32     376

  Fragments arranged by size: 

                1152     376      32      30
 
MscI TGG'CCA

Cuts at:      0    1570    1590 
   Size:       1570      20
 
MslI CAynn'nnrTG

Cuts at:      0      34     277     283     751     925    1120    1285    1590 
   Size:         34     243       6     468     174     195     165     305

  Fragments arranged by size: 

                 468     305     243     195     174     165      34       6
            
 
MspA1I CmG'CkG

Cuts at:      0      93    1256    1590 
   Size:         93    1163     334

  Fragments arranged by size: 

                1163     334      93
 
NcoI C'CATG_G

Cuts at:      0      29    1460    1590 
   Size:         29    1431     130

  Fragments arranged by size: 

                1431     130      29
 
NspI r_CATG'y

Cuts at:      0     282    1545    1590 
   Size:        282    1263      45

  Fragments arranged by size: 

                1263     282      45
 
PasI CC'CwG_GG

Cuts at:      0     348     582    1590 
   Size:        348     234    1008

  Fragments arranged by size: 

                1008     348     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     504    1590 
   Size:        504    1086
 
PfoI T'CCnGG_A

Cuts at:      0     503    1010    1590 
   Size:        503     507     580

  Fragments arranged by size: 

                 580     507     503
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     106     138    1590 
   Size:        106      32    1452

  Fragments arranged by size: 

                1452     106      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     480    1590 
   Size:        480    1110
 
PspOMI G'GGCC_C

Cuts at:      0     351     595    1590 
   Size:        351     244     995

  Fragments arranged by size: 

                 995     351     244
 
PstI C_TGCA'G

Cuts at:      0      14    1590 
   Size:         14    1576
 
PvuII CAG'CTG

Cuts at:      0      93    1590 
   Size:         93    1497
 
SacI G_AGCT'C

Cuts at:      0     311    1590 
   Size:        311    1279
 
ScaI AGT'ACT

Cuts at:      0     895    1590 
   Size:        895     695
 
SexAI A'CCwGG_T

Cuts at:      0     131    1590 
   Size:        131    1459
 
SfcI C'TryA_G

Cuts at:      0      10    1590 
   Size:         10    1580
 
SmlI C'TyrA_G

Cuts at:      0     979    1590 
   Size:        979     611
 
StuI AGG'CCT

Cuts at:      0     109    1370    1590 
   Size:        109    1261     220

  Fragments arranged by size: 

                1261     220     109
 
StyI C'CwwG_G

Cuts at:      0      29      56    1310    1446    1460    1590 
   Size:         29      27    1254     136      14     130

  Fragments arranged by size: 

                1254     136     130      29      27      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     361     850    1590 
   Size:        361     489     740

  Fragments arranged by size: 

                 740     489     361
 
TatI w'GTAC_w

Cuts at:      0     893    1590 
   Size:        893     697
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     388     420    1039    1071    1563    1590 
   Size:        388      32     619      32     492      27

  Fragments arranged by size: 

                 619     492     388      32      32      27
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AloI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI    BciVI     BclI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I     MscI     MslI   MspA1I     NcoI     NspI     PasI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI     ScaI
    SexAI     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII     BmtI     BsaI
    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DrdI     EagI     EciI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com