Restriction map for pF1KB7172
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsaHI       
BsiEI       BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspLU11I    BspMI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRV       FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        KasI        KpnI        KspI        
MfeI        MluI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccI          814  GTAGAC               GT^[AC][GT]AC
AccII         681  CGCG                 CG^CG
AccIII        500  TCCGGA               T^CCGGA
AspHI         328  GAGCTC               G[AT]GC[AT]^C
AspI          775  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          599  TGGCCA               TGG^CCA
BanI          940  GGTGCC               G^G[CT][GA]CC
BanII         328  GAGCTC               G[GA]GC[CT]^C
BbsI         1420  GAAGAC               GAAGAC(2/6)
BfaI          937  CTAG                 C^TAG
BglI          802  GCCACGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          550  GCTAAGC              GC^T[ACGT]AGC
BmyI          328  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      550  GCTAAGC              GC^T[ACGT]AGC
BpuAI        1420  GAAGAC               GAAGAC(2/6)
BsaBI         208  GATCGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          932  GGTCTC               GGTCTC(1/5)
BseAI         500  TCCGGA               T^CCGGA
BsgI         1269  GTGCAG               GTGCAG(16/14)
BsiHKAI       328  GAGCTC               G[AT]GC[AT]^C
BsmBI         446  CGTCTC               CGTCTC(1/5)
BsmFI         398  GGGAC                GGGAC(10/14)
Bsp1286I      328  GAGCTC               G[AGT]GC[ACT]^C
BspEI         500  TCCGGA               T^CCGGA
BspHI        1296  TCATGA               T^CATGA
BsrBI         728  CCGCTC               CCGCTC(-3/-3)
BsrDI         687  CATTGC               GCAATG(2/0)
BssSI         385  CACGAG               CACGAG(-5/-1)
BstEII       1134  GGTCACC              G^GT[ACGT]ACC
BstPI        1134  GGTCACC              G^GT[ACGT]ACC
BstUI         681  CGCG                 CG^CG
BstYI         869  AGATCC               [GA]^GATC[CT]
CelII         550  GCTAAGC              GC^T[ACGT]AGC
EcoO65I      1134  GGTCACC              G^GT[ACGT]ACC
EcoRI         896  GAATTC               G^AATTC
EcoT22I      1167  ATGCAT               ATGCA^T
FauI         1144  GCGGG                CCCGC(4/6)
MaeI          937  CTAG                 C^TAG
MaeII         805  ACGT                 A^CGT
MamI          208  GATCGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          869  AGATCC               [GA]^GATC[CT]
MluNI         599  TGGCCA               TGG^CCA
MroI          500  TCCGGA               T^CCGGA
MscI          599  TGGCCA               TGG^CCA
MspA1I       1336  CAGCGG               C[AC]G^C[GT]G
MvnI          681  CGCG                 CG^CG
NsiI         1167  ATGCAT               ATGCA^T
PflFI         775  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1073  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         926  GACTTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          348  CTGCAG               CTGCA^G
RcaI         1296  TCATGA               T^CATGA
SacI          328  GAGCTC               GAGCT^C
SapI          330  GCTCTTC              GCTCTTC(1/4)
SmlI          573  CTTGAG               C^T[CT][GA]AG
TaqI          308  TCGA                 T^CGA
Tsp45I       1135  GTCAC                ^GT[GC]AC
Tth111I       775  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       308  TCGA                 T^CGA
Van91I       1073  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          630  CCAGAATGAGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         869  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BpmI          160  CTGGAG               CTGGAG(16/14)
              466  CTGGAG              
BsaWI         500  TCCGGA               [AT]^CCGG[AT]
              657  TCCGGT              
DdeI          551  CTAAG                C^T[ACGT]AG
              949  CTGAG               
DsaI          995  CCGTGG               C^C[GA][CT]GG
             1031  CCGTGG              
EaeI          185  TGGCCG               [CT]^GGCC[GA]
              599  TGGCCA              
HincII         49  GTCAAC               GT[CT]^[GA]AC
               82  GTTGAC              
HindII         49  GTCAAC               GT[CT]^[GA]AC
               82  GTTGAC              
HindIII       553  AAGCTT               A^AGCTT
              904  AAGCTT              
MaeIII       1135  GTCAC                ^GT[ACGT]AC
             1439  GTAAC               
MseI           71  TTAA                 T^TAA
             1313  TTAA                
NlaIV         398  GGGACC               GG[ACGT]^[ACGT]CC
              940  GGTGCC              
ScaI          311  AGTACT               AGT^ACT
             1232  AGTACT              
Tru9I          71  TTAA                 T^TAA
             1313  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BcnI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
Cfr13I      DpnII       EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        TseI        
Tsp509I     TspRI       
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