Restriction map for
pF1KB7169
(Circular) (Six-base) MAPSORT of: pF1KB7169.seq Check: 4232 from: 1 to: 4412
pF1KB7169 4412 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1450 1450
Size: 4412
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 4252 4252
Size: 4412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 2237 2669 3416 3684 814
Size: 1423 432 747 268 1542
Fragments arranged by size:
1542 1423 747 432 268
AflIII A'CryG_T
Cuts at: 1764 2868 3845 1764
Size: 1104 977 2331
Fragments arranged by size:
2331 1104 977
AgeI A'CCGG_T
Cuts at: 2800 2800
Size: 4412
AlwNI CAG_nnn'CTG
Cuts at: 179 837 1717 3284 179
Size: 658 880 1567 1307
Fragments arranged by size:
1567 1307 880 658
ApaLI G'TGCA_C
Cuts at: 397 3182 397
Size: 2785 1627
ApoI r'AATT_y
Cuts at: 1416 3765 4021 1416
Size: 2349 256 1807
Fragments arranged by size:
2349 1807 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 1046 1138 1432 1046
Size: 92 294 4026
Fragments arranged by size:
4026 294 92
BamHI G'GATC_C
Cuts at: 206 635 800 1437 206
Size: 429 165 637 3181
Fragments arranged by size:
3181 637 429 165
BanI G'GyrC_C
Cuts at: 1428 1842 2090 2125 1428
Size: 414 248 35 3715
Fragments arranged by size:
3715 414 248 35
BanII G_rGCy'C
Cuts at: 1426 2456 1426
Size: 1030 3382
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BbsI GAAGACnn'nnnn_
Cuts at: 1312 1312
Size: 4412
BbvCI CC'TCA_GC
Cuts at: 550 550
Size: 4412
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3071 4412 2310
Size: 761 1341 2310
Fragments arranged by size:
2310 1341 761
BclI T'GATC_A
Cuts at: 1932 1932
Size: 4412
BglI GCCn_nnn'nGGC
Cuts at: 788 788
Size: 4412
BglII A'GATC_T
Cuts at: 1927 3964 1927
Size: 2037 2375
BlpI GC'TnA_GC
Cuts at: 1363 1524 1363
Size: 161 4251
Bme1580I G_kGCm'C
Cuts at: 392 401 1092 2037 2130 3186 392
Size: 9 691 945 93 1056 1618
Fragments arranged by size:
1618 1056 945 691 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 1624 1705 2040 4184 1624
Size: 81 335 2144 1852
Fragments arranged by size:
2144 1852 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 199 2779 199
Size: 2580 1832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 556 138
Size: 418 3994
Bpu10I CC'TnA_GC
Cuts at: 550 1079 1354 550
Size: 529 275 3608
Fragments arranged by size:
3608 529 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1588 2467 2959 3257 1588
Size: 879 492 298 2743
Fragments arranged by size:
2743 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 964 1219 4116 964
Size: 255 2897 1260
Fragments arranged by size:
2897 1260 255
BsaAI yAC'GTr
Cuts at: 384 1765 384
Size: 1381 3031
BsaBI GATnn'nnATC
Cuts at: 6 211 1926 3963 6
Size: 205 1715 2037 455
Fragments arranged by size:
2037 1715 455 205
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 203 632 803 1601 2122 2800 3074 3221 3632
Size: 429 171 798 521 678 274 147 411
Cuts at: 3632 203
Size: 983
Fragments arranged by size:
983 798 678 521 429 411 274 171
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1264 1294 1264
Size: 30 4382
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 430 457 870 1024 1138 1198 157
Size: 273 27 413 154 114 60 3371
Fragments arranged by size:
3371 413 273 154 114 60 27
BseYI C'CCAG_C
Cuts at: 275 447 1837 2595 3172 3668 275
Size: 172 1390 758 577 496 1019
Fragments arranged by size:
1390 1019 758 577 496 172
BsiEI CG_ry'CG
Cuts at: 104 227 1954 2000 3208 104
Size: 123 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 123 46
BsiHKAI G_wGCw'C
Cuts at: 158 401 1426 2204 2394 3186 158
Size: 243 1025 778 190 792 1384
Fragments arranged by size:
1384 1025 792 778 243 190
BsmBI CGTCTCn'nnnn_
Cuts at: 366 366
Size: 4412
Bsp1286I G_dGCh'C
Cuts at: 158 392 401 1092 1426 2037 2130 2204 2394
Size: 234 9 691 334 611 93 74 190
Cuts at: 2394 2456 3186 158
Size: 62 730 1384
Fragments arranged by size:
1384 730 691 611 334 234 190 93
74 62 9
BspEI T'CCGG_A
Cuts at: 203 632 3632 203
Size: 429 3000 983
Fragments arranged by size:
3000 983 429
BspHI T'CATG_A
Cuts at: 3588 3588
Size: 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1462 1676 1978 2359 1462
Size: 214 302 381 3515
Fragments arranged by size:
3515 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2704 2758 4242 4407 50
Size: 2654 54 1484 165 55
Fragments arranged by size:
2654 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 869 2324 869
Size: 1455 2957
BsrFI r'CCGG_y
Cuts at: 659 2410 2591 2800 659
Size: 1751 181 209 2271
Fragments arranged by size:
2271 1751 209 181
BssSI C'ACGA_G
Cuts at: 2683 3041 2683
Size: 358 4054
BstAPI GCAn_nnn'nTGC
Cuts at: 396 1293 1789 396
Size: 897 496 3019
Fragments arranged by size:
3019 897 496
BstBI TT'CG_AA
Cuts at: 2773 2773
Size: 4412
BstXI CCAn_nnnn'nTGG
Cuts at: 283 681 283
Size: 398 4014
BstYI r'GATC_y
Cuts at: 1 206 635 800 1437 1904 1927 2262 2508
Size: 205 429 165 637 467 23 335 246
Cuts at: 2508 3520 3606 3618 3964 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 637 467 449 429 346 335 246
205 165 86 23 12
BtgI C'CryG_G
Cuts at: 107 704 1178 107
Size: 597 474 3341
Fragments arranged by size:
3341 597 474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2362 2543 2706 2362
Size: 181 163 4068
Fragments arranged by size:
4068 181 163
BtsI GCAGTG_nn'
Cuts at: 979 1063 1655 979
Size: 84 592 3736
Fragments arranged by size:
3736 592 84
DraI TTT'AAA
Cuts at: 1411 1411
Size: 4412
DraIII CAC_nnn'GTG
Cuts at: 396 396
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 2118 2976 2118
Size: 858 3554
EaeI y'GGCC_r
Cuts at: 224 1199 1997 2171 2562 2589 3934 224
Size: 975 798 174 391 27 1345 702
Fragments arranged by size:
1345 975 798 702 391 174 27
EagI C'GGCC_G
Cuts at: 224 1997 224
Size: 1773 2639
EarI CTCTTCn'nnn_
Cuts at: 493 811 914 1253 2435 2645 493
Size: 318 103 339 1182 210 2260
Fragments arranged by size:
2260 1182 339 318 210 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 454 2620 2930 3076 4222 454
Size: 2166 310 146 1146 644
Fragments arranged by size:
2166 1146 644 310 146
EcoICRI GAG'CTC
Cuts at: 1424 1424
Size: 4412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 556 814 2237 2669 3416 3684 138
Size: 418 258 1423 432 747 268 866
Fragments arranged by size:
1423 866 747 432 418 268 258
EcoNI CCTnn'n_nnAGG
Cuts at: 816 816
Size: 4412
EcoO109I rG'GnC_Cy
Cuts at: 542 554 1027 1551 542
Size: 12 473 524 3403
Fragments arranged by size:
3403 524 473 12
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 2431 603
Size: 1828 2584
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 1846 2094 3116 4095 1846
Size: 248 1022 979 2163
Fragments arranged by size:
2163 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 627 659 1252 1264 1284 1296 2254
Size: 32 357 32 593 12 20 12 958
Cuts at: 2254 2284 2286 2316 238
Size: 30 2 30 2334
Fragments arranged by size:
2334 958 593 357 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 1451 1451
Size: 4412
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 3845 3845
Size: 4412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 281 1250 3083 3267 281
Size: 969 1833 184 1426
Fragments arranged by size:
1833 1426 969 184
MscI TGG'CCA
Cuts at: 2173 2173
Size: 4412
MslI CAynn'nnrTG
Cuts at: 2401 2528 2401
Size: 127 4285
MspA1I CmG'CkG
Cuts at: 506 653 1300 1523 1837 2197 3210 3455 506
Size: 147 647 223 314 360 1013 245 1463
Fragments arranged by size:
1463 1013 647 360 314 245 223 147
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 4412
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NcoI C'CATG_G
Cuts at: 107 107
Size: 4412
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 4412
NspI r_CATG'y
Cuts at: 1465 1965 2872 1465
Size: 500 907 3005
Fragments arranged by size:
3005 907 500
PasI CC'CwG_GG
Cuts at: 837 1032 837
Size: 195 4217
PciI A'CATG_T
Cuts at: 2868 2868
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4380
PpuMI rG'GwC_Cy
Cuts at: 542 554 1027 542
Size: 12 473 3927
Fragments arranged by size:
3927 473 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 286 318 286
Size: 32 4380
PstI C_TGCA'G
Cuts at: 350 658 1122 1459 2144 350
Size: 308 464 337 685 2618
Fragments arranged by size:
2618 685 464 337 308
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 1300 1837 2197 1300
Size: 537 360 3515
Fragments arranged by size:
3515 537 360
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 1426 1426
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SanDI GG'GwC_CC
Cuts at: 542 542
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 493 1253 2435 2645 493
Size: 760 1182 210 2260
Fragments arranged by size:
2260 1182 760 210
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 3929 3929
Size: 4412
SfcI C'TryA_G
Cuts at: 33 346 654 824 1118 1455 2140 3133 3324
Size: 313 308 170 294 337 685 993 191
Cuts at: 3324 33
Size: 1121
Fragments arranged by size:
1121 993 685 337 313 308 294 191
170
SfiI GGCCn_nnn'nGGCC
Cuts at: 788 788
Size: 4412
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 1048 1434 1048
Size: 386 4026
SmlI C'TyrA_G
Cuts at: 1567 2482 2974 3236 1567
Size: 915 492 262 2743
Fragments arranged by size:
2743 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 1465 1965 1465
Size: 500 3912
StuI AGG'CCT
Cuts at: 313 822 313
Size: 509 3903
StyI C'CwwG_G
Cuts at: 107 216 896 1546 1900 107
Size: 109 680 650 354 2619
Fragments arranged by size:
2619 680 650 354 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 330 967 1995 2128 2587 2796 289
Size: 41 637 1028 133 459 209 1905
Fragments arranged by size:
1905 1028 637 459 209 133 41
TatI w'GTAC_w
Cuts at: 3927 3927
Size: 4412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 4380
Tth111I GACn'n_nGTC
Cuts at: 2209 2209
Size: 4412
XbaI T'CTAG_A
Cuts at: 1443 1443
Size: 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1907 1907
Size: 4412
XmaI C'CCGG_G
Cuts at: 1046 1432 1046
Size: 386 4026
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI SacII SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI