Restriction map for pF1KB7162
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvrII       BalI        
BamHI       BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BpuAI       BsaBI       BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HaeII       HindIII     
HinfI       HpaI        KasI        KpnI        MaeI        
MamI        MfeI        MluI        MluNI       MscI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SbfI        ScaI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        425  TCCGGA               T^CCGGA
AflIII        764  ACGTGT               A^C[GA][CT]GT
AhdI          283  GACAACTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          705  GATCC                GGATC(4/5)
ApaI         1025  GGGCCC               GGGCC^C
AspEI         283  GACAACTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         779  TGCGCA               TGC^GCA
BbrPI         763  CACGTG               CAC^GTG
BlpI          720  GCTCAGC              GC^T[ACGT]AGC
BpmI          960  CTCCAG               CTGGAG(16/14)
Bpu1102I      720  GCTCAGC              GC^T[ACGT]AGC
BsaWI         425  TCCGGA               [AT]^CCGG[AT]
BseAI         425  TCCGGA               T^CCGGA
BspEI         425  TCCGGA               T^CCGGA
BsrFI         967  ACCGGC               [GA]^CCGG[CT]
BssSI         505  CACGAG               CACGAG(-5/-1)
BstAPI        264  GCAGTACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         704  AGATCC               [GA]^GATC[CT]
CelII         720  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        967  ACCGGC               [GA]^CCGG[CT]
DdeI          721  CTCAG                C^T[ACGT]AG
DraIII        535  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      283  GACAACTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         956  CCTGCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          779  TGCGCA               TGC^GCA
HincII       1050  GTTGAC               GT[CT]^[GA]AC
HindII       1050  GTTGAC               GT[CT]^[GA]AC
KspI          930  CCGCGG               CCGC^GG
MaeIII        115  GTTAC                ^GT[ACGT]AC
MflI          704  AGATCC               [GA]^GATC[CT]
MroI          425  TCCGGA               T^CCGGA
NlaIII        569  CATG                 CATG^
PmaCI         763  CACGTG               CAC^GTG
PmlI          763  CACGTG               CAC^GTG
PpuMI         914  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         798  CAGCTG               CAG^CTG
SacII         930  CCGCGG               CCGC^GG
SapI          687  GAAGAGC              GCTCTTC(1/4)
TspRI          42  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI         1109  CCACGGGCAGGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         704  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          448  GGCGTC               G[GA]^CG[CT]C
              636  GGCGTC              
AvaI         1003  CCCGAG               C^[CT]CG[GA]G
             1042  CCCGAG              
BanI          679  GGCACC               G^G[CT][GA]CC
              997  GGTGCC              
BanII        1025  GGGCCC               G[GA]GC[CT]^C
             1039  GAGCCC              
BsaAI         268  TACGTG               [CT]AC^GT[GA]
              763  CACGTG              
BsaHI         448  GGCGTC               G[GA]^CG[CT]C
              636  GGCGTC              
BsaI          901  GAGACC               GGTCTC(1/5)
              290  GGTCTC              
BsiEI         440  CGGCCG               CG[GA][CT]^CG
              969  CGGCCG              
BsmAI         901  GAGAC                GTCTC(1/5)
              291  GTCTC               
BsmFI         914  GGGAC                GGGAC(10/14)
              153  GTCCC               
BsoBI        1003  CCCGAG               C^[CT]CG[GA]G
             1042  CCCGAG              
BsrI          287  ACTGG                ACTGG(1/-1)
              420  CCAGT               
BssHII        576  GCGCGC               G^CGCGC
              657  GCGCGC              
DpnII         177  GATC                 ^GATC
              705  GATC                
EagI          440  CGGCCG               C^GGCCG
              969  CGGCCG              
EclXI         440  CGGCCG               C^GGCCG
              969  CGGCCG              
Eco52I        440  CGGCCG               C^GGCCG
              969  CGGCCG              
EcoT14I       470  CCAAGG               C^C[AT][AT]GG
              812  CCAAGG              
Hin1I         448  GGCGTC               G[GA]^CG[CT]C
              636  GGCGTC              
MaeII         269  ACGT                 A^CGT
              764  ACGT                
MboI          177  GATC                 ^GATC
              705  GATC                
MslI          348  CATCAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              372  CATCTCCGTG          
NdeII         177  GATC                 ^GATC
              705  GATC                
PflMI          90  CCAGACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              708  CCAGCGGCTGG         
Sau3AI        177  GATC                 ^GATC
              705  GATC                
SfiI          936  GGCCTGCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              975  GGCCAGGGAGGCC       
StuI          983  AGGCCT               AGG^CCT
             1007  AGGCCT              
StyI          470  CCAAGG               C^C[AT][AT]GG
              812  CCAAGG              
Van91I         90  CCAGACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              708  CCAGCGGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BbvI        BcnI        BglI        
BmyI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BspMI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
HphI        ItaI        Ksp632I     MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIV       PstI        RsaI        Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
TthHB8I     
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