Restriction map for pF1KB7162
 (Linear) (Six-base) MAPSORT of: KIBB7162.seq  Check: 5386  from: 1  to: 1125



KIBB7162.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     964    1125 
   Size:        964     161
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     454    1125 
   Size:          2     452     671

  Fragments arranged by size: 

                 671     452       2
 
AflIII A'CryG_T

Cuts at:      0     764    1125 
   Size:        764     361
 
AhdI GACnn_n'nnGTC

Cuts at:      0     288    1125 
   Size:        288     837
 
AlwNI CAG_nnn'CTG

Cuts at:      0      96     714     788    1125 
   Size:         96     618      74     337

  Fragments arranged by size: 

                 618     337      96      74
 
ApaI G_GGCC'C

Cuts at:      0    1029    1125 
   Size:       1029      96
 
AvaI C'yCGr_G

Cuts at:      0    1003    1042    1125 
   Size:       1003      39      83

  Fragments arranged by size: 

                1003      83      39
 
BanI G'GyrC_C

Cuts at:      0     679     997    1125 
   Size:        679     318     128

  Fragments arranged by size: 

                 679     318     128
 
BanII G_rGCy'C

Cuts at:      0    1029    1043    1125 
   Size:       1029      14      82

  Fragments arranged by size: 

                1029      82      14
 
BglI GCCn_nnn'nGGC

Cuts at:      0     258     943     982    1006    1125 
   Size:        258     685      39      24     119

  Fragments arranged by size: 

                 685     258     119      39      24
 
BlpI GC'TnA_GC

Cuts at:      0     721    1125 
   Size:        721     404
 
Bme1580I G_kGCm'C

Cuts at:      0    1002    1029    1125 
   Size:       1002      27      96

  Fragments arranged by size: 

                1002      96      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     945    1125 
   Size:        945     180
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     296     895    1125 
   Size:        296     599     230

  Fragments arranged by size: 

                 599     296     230
 
BsaAI yAC'GTr

Cuts at:      0     270     765    1125 
   Size:        270     495     360

  Fragments arranged by size: 

                 495     360     270
 
BsaHI Gr'CG_yC

Cuts at:      0     449     637    1125 
   Size:        449     188     488

  Fragments arranged by size: 

                 488     449     188
 
BsaWI w'CCGG_w

Cuts at:      0     425    1125 
   Size:        425     700
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     823     853    1125 
   Size:        823      30     272

  Fragments arranged by size: 

                 823     272      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18      45     523    1051    1125 
   Size:         18      27     478     528      74

  Fragments arranged by size: 

                 528     478      74      27      18
 
BseYI C'CCAG_C

Cuts at:      0     195     993    1125 
   Size:        195     798     132

  Fragments arranged by size: 

                 798     195     132
 
BsiEI CG_ry'CG

Cuts at:      0     443     972    1125 
   Size:        443     529     153

  Fragments arranged by size: 

                 529     443     153
 
BsiHKAI G_wGCw'C

Cuts at:      0     554     722     884    1125 
   Size:        554     168     162     241

  Fragments arranged by size: 

                 554     241     168     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     554     722     884    1002    1029    1043    1125 
   Size:        554     168     162     118      27      14      82

  Fragments arranged by size: 

                 554     168     162     118      82      27      14
 
BspEI T'CCGG_A

Cuts at:      0     425    1125 
   Size:        425     700
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     179     964    1106    1125 
   Size:        179     785     142      19

  Fragments arranged by size: 

                 785     179     142      19
 
BsrFI r'CCGG_y

Cuts at:      0     967    1125 
   Size:        967     158
 
BssHII G'CGCG_C

Cuts at:      0     576     657    1125 
   Size:        576      81     468

  Fragments arranged by size: 

                 576     468      81
 
BssSI C'ACGA_G

Cuts at:      0     505    1125 
   Size:        505     620
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     270    1125 
   Size:        270     855
 
BstYI r'GATC_y

Cuts at:      0     704    1125 
   Size:        704     421
 
BtgI C'CryG_G

Cuts at:      0     377     675     728     930    1109    1125 
   Size:        377     298      53     202     179      16

  Fragments arranged by size: 

                 377     298     202     179      53      16
 
BtsI GCAGTG_nn'

Cuts at:      0      41    1125 
   Size:         41    1084
 
DraIII CAC_nnn'GTG

Cuts at:      0     540    1125 
   Size:        540     585
 
EaeI y'GGCC_r

Cuts at:      0     140     224     440     969    1125 
   Size:        140      84     216     529     156

  Fragments arranged by size: 

                 529     216     156     140      84
 
EagI C'GGCC_G

Cuts at:      0     440     969    1125 
   Size:        440     529     156

  Fragments arranged by size: 

                 529     440     156
 
EarI CTCTTCn'nnn_

Cuts at:      0     238     619     682    1125 
   Size:        238     381      63     443

  Fragments arranged by size: 

                 443     381     238      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     454     945    1125 
   Size:          2     452     491     180

  Fragments arranged by size: 

                 491     452     180       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     960    1125 
   Size:        960     165
 
EcoO109I rG'GnC_Cy

Cuts at:      0     915     935    1025    1125 
   Size:        915      20      90     100

  Fragments arranged by size: 

                 915     100      90      20
 
FspI TGC'GCA

Cuts at:      0     781    1125 
   Size:        781     344
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      40      72     274     306    1125 
   Size:         40      32     202      32     819

  Fragments arranged by size: 

                 819     202      40      32      32
 
HincII GTy'rAC

Cuts at:      0    1052    1125 
   Size:       1052      73
 
MslI CAynn'nnrTG

Cuts at:      0     352     376    1125 
   Size:        352      24     749

  Fragments arranged by size: 

                 749     352      24
 
MspA1I CmG'CkG

Cuts at:      0     711     800     932     993    1125 
   Size:        711      89     132      61     132

  Fragments arranged by size: 

                 711     132     132      89      61
 
PasI CC'CwG_GG

Cuts at:      0    1029    1125 
   Size:       1029      96
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      96     714    1125 
   Size:         96     618     411

  Fragments arranged by size: 

                 618     411      96
 
PfoI T'CCnGG_A

Cuts at:      0     961    1012    1125 
   Size:        961      51     113

  Fragments arranged by size: 

                 961     113      51
 
PmlI CAC'GTG

Cuts at:      0     765    1125 
   Size:        765     360
 
PpuMI rG'GwC_Cy

Cuts at:      0     915    1125 
   Size:        915     210
 
PspOMI G'GGCC_C

Cuts at:      0    1025    1125 
   Size:       1025     100
 
PstI C_TGCA'G

Cuts at:      0      48     176     266     278    1125 
   Size:         48     128      90      12     847

  Fragments arranged by size: 

                 847     128      90      48      12
 
PvuII CAG'CTG

Cuts at:      0     800    1125 
   Size:        800     325
 
SacII CC_GC'GG

Cuts at:      0     933    1125 
   Size:        933     192
 
SapI GCTCTTCn'nnn_

Cuts at:      0     682    1125 
   Size:        682     443
 
SfcI C'TryA_G

Cuts at:      0      44     172     262     274    1125 
   Size:         44     128      90      12     851

  Fragments arranged by size: 

                 851     128      90      44      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     943     982    1125 
   Size:        943      39     143

  Fragments arranged by size: 

                 943     143      39
 
StuI AGG'CCT

Cuts at:      0     985    1009    1125 
   Size:        985      24     116

  Fragments arranged by size: 

                 985     116      24
 
StyI C'CwwG_G

Cuts at:      0     470     812    1125 
   Size:        470     342     313

  Fragments arranged by size: 

                 470     342     313
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     697     922     985    1125 
   Size:        697     225      63     140

  Fragments arranged by size: 

                 697     225     140      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1116    1125 
   Size:       1116       9
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI    AlwNI     ApaI     AvaI     BanI
    BanII     BglI     BlpI Bme1580I     BpmI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstYI     BtgI     BtsI
   DraIII     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I     FspI
    Hin4I   HincII     MslI   MspA1I     PasI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII    SacII     SapI     SfcI     SfiI
     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaBI     BsgI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI
    BsrGI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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