Restriction map for pF1KB7130
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstZ17I     Cac8I       CelII       
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       FauI        
FbaI        FokI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AfaI          847  GTAC                 GT^AC
AlwI          121  GGATC                GGATC(4/5)
AocI          870  CCTTAGG              CC^T[ACGT]AGG
Asp700I       185  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          818  TGGCCA               TGG^CCA
BfaI          277  CTAG                 C^TAG
BsaWI         809  ACCGGA               [AT]^CCGG[AT]
BsmI          468  GCATTC               GAATGC(1/-1)
BsrBI         541  CCGCTC               CCGCTC(-3/-3)
BssSI         247  CTCGTG               CACGAG(-5/-1)
BstXI         329  CCATTTTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         121  GGATCT               [GA]^GATC[CT]
Bsu36I        870  CCTTAGG              CC^T[ACGT]AGG
CfoI          833  GCGC                 GCG^C
DpnII         122  GATC                 ^GATC
DraI          925  TTTAAA               TTT^AAA
DraII         682  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          614  CCATGG               C^C[GA][CT]GG
EaeI          818  TGGCCA               [CT]^GGCC[GA]
Eco81I        870  CCTTAGG              CC^T[ACGT]AGG
EcoO109I      682  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       614  CCATGG               C^C[AT][AT]GG
EcoT22I       466  ATGCAT               ATGCA^T
HaeII         832  AGCGCC               [GA]GCGC^[CT]
HapII         810  CCGG                 C^CGG
HhaI          833  GCGC                 GCG^C
HinP1I        833  GCGC                 G^CGC
HpaII         810  CCGG                 C^CGG
MaeI          277  CTAG                 C^TAG
MboI          122  GATC                 ^GATC
MflI          121  GGATCT               [GA]^GATC[CT]
MluNI         818  TGGCCA               TGG^CCA
MscI          818  TGGCCA               TGG^CCA
MspA1I        428  CAGCTG               C[AC]G^C[GT]G
MspI          810  CCGG                 C^CGG
NcoI          614  CCATGG               C^CATGG
NdeII         122  GATC                 ^GATC
NlaIV         147  GGAACC               GG[ACGT]^[ACGT]CC
NsiI          466  ATGCAT               ATGCA^T
PleI          633  GACTC                GAGTC(4/5)
PpuMI         682  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         428  CAGCTG               CAG^CTG
RsaI          847  GTAC                 GT^AC
Sau3AI        122  GATC                 ^GATC
SfaNI         465  GATGC                GCATC(5/9)
StyI          614  CCATGG               C^C[AT][AT]GG
XhoII         121  GGATCT               [GA]^GATC[CT]
XmnI          185  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI          541  CCGC                 CCGC(-3/-1)
              433  GCGG                
AlwNI          55  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              681  CAGGTCCTG           
BsaJI         407  CCCAGG               C^C[ACGT][ACGT]GG
              614  CCATGG              
BsgI          482  CTGCAC               GTGCAG(16/14)
              252  GTGCAG              
BspHI         398  TCATGA               T^CATGA
              476  TCATGA              
BssKI         408  CCAGG                ^CC[ACGT]GG
              437  CCTGG               
BstNI         408  CCAGG                CC^[AT]GG
              437  CCTGG               
EarI          380  CTCTTC               CTCTTC(1/4)
              635  CTCTTC              
EcoRII        408  CCAGG                ^CC[AT]GG
              437  CCTGG               
Ksp632I       380  CTCTTC               CTCTTC(1/4)
              635  CTCTTC              
MvaI          408  CCAGG                CC^[AT]GG
              437  CCTGG               
PflMI         306  CCACTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              726  CCATTTTATGG         
PstI           61  CTGCAG               CTGCA^G
              687  CTGCAG              
RcaI          398  TCATGA               T^CATGA
              476  TCATGA              
ScrFI         408  CCAGG                CC^[ACGT]GG
              437  CCTGG               
TfiI          801  GATTC                G^A[AT]TC
              852  GAATC               
Tsp509I       551  AATT                 ^AATT
              657  AATT                
Van91I        306  CCACTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              726  CCATTTTATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AluI        AvaII       BbvI        BsiYI       BslI        
BsrI        Cfr13I      DdeI        Fnu4HI      HaeIII      
HinfI       HphI        ItaI        MaeIII      MboII       
MnlI        MseI        MslI        MwoI        NlaIII      
Sau96I      SfcI        Tru9I       TseI        Tsp45I      
TspRI       
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