Restriction map for pF1KB7122
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SwaI        
TaqI        TfiI        Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          241  GTAGAC               GT^[AC][GT]AC
AcsI           25  GAATTC               [GA]^AATT[CT]
AfaI          765  GTAC                 GT^AC
AlwI          326  GGATC                GGATC(4/5)
AlwNI         151  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           25  GAATTC               [GA]^AATT[CT]
Asp700I        25  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         258  GAGCAC               G[AT]GC[AT]^C
AvaII         502  GGTCC                G^G[AT]CC
BmyI          258  GAGCAC               G[AGT]GC[ACT]^C
BpmI           55  CTGGAG               CTGGAG(16/14)
BsaAI         361  TACGTG               [CT]AC^GT[GA]
BsaI           40  GGTCTC               GGTCTC(1/5)
BseRI         334  CTCCTC               GAGGAG(10/8)
BsiHKAI       258  GAGCAC               G[AT]GC[AT]^C
BsmAI          41  GTCTC                GTCTC(1/5)
BsmI          864  GAATGC               GAATGC(1/-1)
Bsp1286I      258  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I      764  TGTACA               T^GTACA
BspHI         398  TCATGA               T^CATGA
BspMI         709  ACCTGC               ACCTGC(4/8)
BsrGI         764  TGTACA               T^GTACA
BstEII        161  GGTCACC              G^GT[ACGT]ACC
BstPI         161  GGTCACC              G^GT[ACGT]ACC
BstXI         104  CCACAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI          406  CCACGG               C^C[GA][CT]GG
EarI          475  CTCTTC               CTCTTC(1/4)
EcoO65I       161  GGTCACC              G^GT[ACGT]ACC
EcoRI          25  GAATTC               G^AATTC
EcoT14I       230  CCAAGG               C^C[AT][AT]GG
HgaI          640  GCGTC                GACGC(5/10)
HincII        546  GTTGAC               GT[CT]^[GA]AC
HindII        546  GTTGAC               GT[CT]^[GA]AC
Ksp632I       475  CTCTTC               CTCTTC(1/4)
MaeII         362  ACGT                 A^CGT
MspA1I        292  CAGCTG               C[AC]G^C[GT]G
PflMI         570  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          711  CTGCAG               CTGCA^G
PvuII         292  CAGCTG               CAG^CTG
RcaI          398  TCATGA               T^CATGA
RsaI          765  GTAC                 GT^AC
SfcI          711  CTGCAG               C^T[GA][CT]AG
SspBI         764  TGTACA               T^GTACA
SspI           69  AATATT               AAT^ATT
StuI          772  AGGCCT               AGG^CCT
StyI          230  CCAAGG               C^C[AT][AT]GG
Van91I        570  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI           25  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI          357  CCGC                 CCGC(-3/-1)
              690  GCGG                
BalI          365  TGGCCA               TGG^CCA
              617  TGGCCA              
BsgI          382  CTGCAC               GTGCAG(16/14)
              558  CTGCAC              
BsmFI         192  GTCCC                GGGAC(10/14)
              748  GTCCC               
BsrDI         460  GCAATG               GCAATG(2/0)
              802  GCAATG              
BssKI          54  CCTGG                ^CC[ACGT]GG
              576  CCTGG               
BstNI          54  CCTGG                CC^[AT]GG
              576  CCTGG               
Cac8I         456  GCACGC               GC[ACGT]^[ACGT]GC
              556  GCCTGC              
Cfr13I        502  GGTCC                G^G[ACGT]CC
              579  GGGCC               
EaeI          365  TGGCCA               [CT]^GGCC[GA]
              617  TGGCCA              
EcoRII         54  CCTGG                ^CC[AT]GG
              576  CCTGG               
MluNI         365  TGGCCA               TGG^CCA
              617  TGGCCA              
MscI          365  TGGCCA               TGG^CCA
              617  TGGCCA              
MseI          634  TTAA                 T^TAA
              838  TTAA                
MvaI           54  CCTGG                CC^[AT]GG
              576  CCTGG               
Sau96I        502  GGTCC                G^G[ACGT]CC
              579  GGGCC               
ScrFI          54  CCTGG                CC^[ACGT]GG
              576  CCTGG               
Tru9I         634  TTAA                 T^TAA
              838  TTAA                
Tsp509I        26  AATT                 ^AATT
              636  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BstF5I      DdeI        DpnII       Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MslI        NdeII       
NlaIII      PleI        Sau3AI      TseI        Tsp45I      
TspRI       
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