Restriction map for pF1KB7118
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      CelII       
CpoI        DpnII       DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HincII      HindII      
HindIII     HpaI        KasI        KpnI        MaeI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         342  CGCG                 CG^CG
AcyI           70  GACGCC               G[GA]^CG[CT]C
AhdI          662  GACATGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         662  GACATGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         705  GTGCTC               G[AT]GC[AT]^C
BsaBI         324  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          70  GACGCC               G[GA]^CG[CT]C
BsaI          764  GGTCTC               GGTCTC(1/5)
BsgI          566  CTGCAC               GTGCAG(16/14)
BsiHKAI       705  GTGCTC               G[AT]GC[AT]^C
BspDI         195  ATCGAT               AT^CGAT
BsrBI          75  CCGCTC               CCGCTC(-3/-3)
BsrFI         736  GCCGGT               [GA]^CCGG[CT]
BstUI         342  CGCG                 CG^CG
Cfr10I        736  GCCGGT               [GA]^CCGG[CT]
ClaI          195  ATCGAT               AT^CGAT
DsaI          341  CCGCGG               C^C[GA][CT]GG
Eam1105I      662  GACATGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          619  GAAGAG               CTCTTC(1/4)
EcoT14I       747  CCAAGG               C^C[AT][AT]GG
HgaI           70  GACGC                GACGC(5/10)
Hin1I          70  GACGCC               G[GA]^CG[CT]C
HinfI         499  GATTC                G^A[ACGT]TC
Ksp632I       619  GAAGAG               CTCTTC(1/4)
KspI          341  CCGCGG               CCGC^GG
MaeIII        422  GTGAC                ^GT[ACGT]AC
MamI          324  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        341  CCGCGG               C[AC]G^C[GT]G
MvnI          342  CGCG                 CG^CG
NlaIII        664  CATG                 CATG^
SacII         341  CCGCGG               CCGC^GG
SfaNI         285  GATGC                GCATC(5/9)
SmaI          440  CCCGGG               CCC^GGG
StuI          368  AGGCCT               AGG^CCT
StyI          747  CCAAGG               C^C[AT][AT]GG
TaqI          196  TCGA                 T^CGA
TfiI          499  GATTC                G^A[AT]TC
Tsp45I        422  GTGAC                ^GT[GC]AC
TthHB8I       196  TCGA                 T^CGA
XmaCI         440  CCCGGG               C^CCGGG
XmaI          440  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI          279  GTAC                 GT^AC
              408  GTAC                
AluI          512  AGCT                 AG^CT
              755  AGCT                
BbvCI         183  CCTCAGC              CCTCAGC(-5/-2)
              371  CCTCAGC             
BpmI          514  CTCCAG               CTGGAG(16/14)
              103  CTGGAG              
BsaAI         280  TACGTG               [CT]AC^GT[GA]
              409  TACGTG              
BsmAI         679  GAGAC                GTCTC(1/5)
              765  GTCTC               
CfoI          611  GCGC                 GCG^C
              691  GCGC                
DrdI          646  GACCAGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              794  GACCAGTGGGTC        
EaeI          530  TGGCCG               [CT]^GGCC[GA]
              734  TGGCCG              
HhaI          611  GCGC                 GCG^C
              691  GCGC                
HinP1I        611  GCGC                 G^CGC
              691  GCGC                
MslI          192  CACATCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              838  CACCCCAGTG          
RsaI          279  GTAC                 GT^AC
              408  GTAC                
SmlI          547  CTCAAG               C^T[CT][GA]AG
              718  CTTGAG              
XcmI          239  CCACCAACTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              560  CCAGGGCTGCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        ApaI        AvaI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HpaII       HphI        ItaI        MaeII       
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NlaIV       PpuMI       Sau96I      ScrFI       
TseI        TspRI       
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