Restriction map for pF1KB7114
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        AspEI       AspI        AvaII       AviII       
AvrII       BamHI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        HindIII     HpaI        KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   75 enzymes) #
###################################

Acc65I        697  GGTACC               G^GTACC
AccII          94  CGCG                 CG^CG
AcyI          341  GGCGCC               G[GA]^CG[CT]C
Alw44I        675  GTGCAC               G^TGCAC
AlwI          499  GATCC                GGATC(4/5)
ApaI          230  GGGCCC               GGGCC^C
ApaLI         675  GTGCAC               G^TGCAC
Asp700I       549  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       697  GGTACC               G^GTACC
AvaI          791  CCCGAG               C^[CT]CG[GA]G
BalI          572  TGGCCA               TGG^CCA
BbeI          341  GGCGCC               GGCGC^C
BbrPI         280  CACGTG               CAC^GTG
BbsI           82  GTCTTC               GAAGAC(2/6)
BfaI          403  CTAG                 C^TAG
BglI          167  GCCAGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI            6  CTGGAG               CTGGAG(16/14)
BpuAI          82  GTCTTC               GAAGAC(2/6)
BsaAI         280  CACGTG               [CT]AC^GT[GA]
BsaHI         341  GGCGCC               G[GA]^CG[CT]C
BsaI          794  GAGACC               GGTCTC(1/5)
BseRI         770  CTCCTC               GAGGAG(10/8)
BsiEI         116  CGGCCG               CG[GA][CT]^CG
BsoBI         791  CCCGAG               C^[CT]CG[GA]G
BsrI            5  ACTGG                ACTGG(1/-1)
BstAPI        629  GCAAGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        736  GGTGACC              G^GT[ACGT]ACC
BstPI         736  GGTGACC              G^GT[ACGT]ACC
BstUI          94  CGCG                 CG^CG
BstXI         509  CCAATATAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         498  AGATCC               [GA]^GATC[CT]
DpnII         499  GATC                 ^GATC
DraII         230  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EagI          116  CGGCCG               C^GGCCG
EclXI         116  CGGCCG               C^GGCCG
Eco52I        116  CGGCCG               C^GGCCG
EcoO109I      230  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       736  GGTGACC              G^GT[ACGT]ACC
EcoT14I       324  CCATGG               C^C[AT][AT]GG
FauI          369  GCGGG                CCCGC(4/6)
HaeII         341  GGCGCC               [GA]GCGC^[CT]
Hin1I         341  GGCGCC               G[GA]^CG[CT]C
HincII         37  GTCAAC               GT[CT]^[GA]AC
HindII         37  GTCAAC               GT[CT]^[GA]AC
HinfI          23  GACTC                G^A[ACGT]TC
KasI          341  GGCGCC               G^GCGCC
KpnI          697  GGTACC               GGTAC^C
MaeI          403  CTAG                 C^TAG
MboI          499  GATC                 ^GATC
MflI          498  AGATCC               [GA]^GATC[CT]
MluNI         572  TGGCCA               TGG^CCA
MscI          572  TGGCCA               TGG^CCA
MslI          276  CATGCACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        578  CAGCTG               C[AC]G^C[GT]G
MvnI           94  CGCG                 CG^CG
NarI          341  GGCGCC               GG^CGCC
NcoI          324  CCATGG               C^CATGG
NdeI          389  CATATG               CA^TATG
NdeII         499  GATC                 ^GATC
NspI          253  ACATGC               [GA]CATG^[CT]
PleI           23  GACTC                GAGTC(4/5)
PmaCI         280  CACGTG               CAC^GTG
PmlI          280  CACGTG               CAC^GTG
PshAI         387  GACATATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         578  CAGCTG               CAG^CTG
SacI          610  GAGCTC               GAGCT^C
Sau3AI        499  GATC                 ^GATC
ScaI           44  AGTACT               AGT^ACT
SfoI          341  GGCGCC               GGC^GCC
SmlI          665  CTCAAG               C^T[CT][GA]AG
StyI          324  CCATGG               C^C[AT][AT]GG
TaqI          152  TCGA                 T^CGA
TthHB8I       152  TCGA                 T^CGA
XhoII         498  AGATCC               [GA]^GATC[CT]
XmnI          549  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspHI         610  GAGCTC               G[AT]GC[AT]^C
              675  GTGCAC              
BsiHKAI       610  GAGCTC               G[AT]GC[AT]^C
              675  GTGCAC              
BsmAI         794  GAGAC                GTCTC(1/5)
               75  GTCTC               
BstF5I        591  CATCC                GGATG(2/0)
              766  CATCC               
Cfr13I        230  GGGCC                G^G[ACGT]CC
              231  GGCCC               
DsaI          324  CCATGG               C^C[GA][CT]GG
              693  CCACGG              
EaeI          116  CGGCCG               [CT]^GGCC[GA]
              572  TGGCCA              
FokI          591  CATCC                GGATG(9/13)
              766  CATCC               
MaeII         185  ACGT                 A^CGT
              281  ACGT                
PstI          235  CTGCAG               CTGCA^G
              396  CTGCAG              
Sau96I        230  GGGCC                G^G[ACGT]CC
              231  GGCCC               
SexAI         107  ACCTGGT              A^CC[AT]GGT
              620  ACCTGGT             
SfcI          235  CTGCAG               C^T[GA][CT]AG
              396  CTGCAG              
TspRI         263  CACTG                CA[GC]TG[ACGT][ACGT]^
              336  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AfaI        AluI        BanI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BssSI       
BstNI       Cac8I       CfoI        DdeI        EarI        
EcoRII      Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboII       MnlI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       RsaI        
ScrFI       SfaNI       TseI        Tsp45I      
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