Restriction map for pF1KB7114
 (Linear) (Six-base) MAPSORT of: KIBB7114.seq  Check: 5425  from: 1  to: 819



KIBB7114.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

Acc65I G'GTAC_C

Cuts at:      0     697     819 
   Size:        697     122
 
ApaI G_GGCC'C

Cuts at:      0     234     819 
   Size:        234     585
 
ApaLI G'TGCA_C

Cuts at:      0     675     819 
   Size:        675     144
 
AvaI C'yCGr_G

Cuts at:      0     791     819 
   Size:        791      28
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      27     819 
   Size:         27     792
 
BanI G'GyrC_C

Cuts at:      0     311     341     697     819 
   Size:        311      30     356     122

  Fragments arranged by size: 

                 356     311     122      30
 
BanII G_rGCy'C

Cuts at:      0     234     380     614     757     819 
   Size:        234     146     234     143      62

  Fragments arranged by size: 

                 234     234     146     143      62
 
BbeI G_GCGC'C

Cuts at:      0     345     819 
   Size:        345     474
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      75     819 
   Size:         75     744
 
BglI GCCn_nnn'nGGC

Cuts at:      0     173     819 
   Size:        173     646
 
Bme1580I G_kGCm'C

Cuts at:      0     234     679     819 
   Size:        234     445     140

  Fragments arranged by size: 

                 445     234     140
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     819 
   Size:         27     792
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     650     819 
   Size:        650     169
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     788     819 
   Size:        788      31
 
BsaAI yAC'GTr

Cuts at:      0     282     819 
   Size:        282     537
 
BsaHI Gr'CG_yC

Cuts at:      0     342     819 
   Size:        342     477
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61     819 
   Size:         31      30     758

  Fragments arranged by size: 

                 758      31      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     761     819 
   Size:        761      58
 
BsiEI CG_ry'CG

Cuts at:      0     119     819 
   Size:        119     700
 
BsiHKAI G_wGCw'C

Cuts at:      0     614     679     819 
   Size:        614      65     140

  Fragments arranged by size: 

                 614     140      65
 
Bsp1286I G_dGCh'C

Cuts at:      0     234     380     614     679     757     819 
   Size:        234     146     234      65      78      62

  Fragments arranged by size: 

                 234     234     146      78      65      62
 
BssSI C'ACGA_G

Cuts at:      0     268     604     640     819 
   Size:        268     336      36     179

  Fragments arranged by size: 

                 336     268     179      36
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     635     819 
   Size:        635     184
 
BstEII G'GTnAC_C

Cuts at:      0     736     819 
   Size:        736      83
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     516     819 
   Size:        516     303
 
BstYI r'GATC_y

Cuts at:      0     498     819 
   Size:        498     321
 
BtgI C'CryG_G

Cuts at:      0     324     693     819 
   Size:        324     369     126

  Fragments arranged by size: 

                 369     324     126
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     411     819 
   Size:        411     408
 
BtsI GCAGTG_nn'

Cuts at:      0     262     819 
   Size:        262     557
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      39      74     819 
   Size:         39      35     745

  Fragments arranged by size: 

                 745      39      35
 
EaeI y'GGCC_r

Cuts at:      0     116     572     819 
   Size:        116     456     247

  Fragments arranged by size: 

                 456     247     116
 
EagI C'GGCC_G

Cuts at:      0     116     819 
   Size:        116     703
 
EarI CTCTTCn'nnn_

Cuts at:      0     301     568     711     819 
   Size:        301     267     143     108

  Fragments arranged by size: 

                 301     267     143     108
 
EcoICRI GAG'CTC

Cuts at:      0     612     819 
   Size:        612     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     819 
   Size:         27     792
 
EcoO109I rG'GnC_Cy

Cuts at:      0     231     819 
   Size:        231     588
 
HaeII r_GCGC'y

Cuts at:      0     345     819 
   Size:        345     474
 
HincII GTy'rAC

Cuts at:      0      39     819 
   Size:         39     780
 
KasI G'GCGC_C

Cuts at:      0     341     819 
   Size:        341     478
 
KpnI G_GTAC'C

Cuts at:      0     701     819 
   Size:        701     118
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     819 
   Size:        206     613
 
MscI TGG'CCA

Cuts at:      0     574     819 
   Size:        574     245
 
MslI CAynn'nnrTG

Cuts at:      0     280     819 
   Size:        280     539
 
MspA1I CmG'CkG

Cuts at:      0     580     819 
   Size:        580     239
 
NarI GG'CG_CC

Cuts at:      0     342     819 
   Size:        342     477
 
NcoI C'CATG_G

Cuts at:      0     324     819 
   Size:        324     495
 
NdeI CA'TA_TG

Cuts at:      0     390     819 
   Size:        390     429
 
NspI r_CATG'y

Cuts at:      0     257     819 
   Size:        257     562
 
PmlI CAC'GTG

Cuts at:      0     282     819 
   Size:        282     537
 
PshAI GACnn'nnGTC

Cuts at:      0     391     819 
   Size:        391     428
 
PspOMI G'GGCC_C

Cuts at:      0     230     819 
   Size:        230     589
 
PstI C_TGCA'G

Cuts at:      0     239     400     819 
   Size:        239     161     419

  Fragments arranged by size: 

                 419     239     161
 
PvuII CAG'CTG

Cuts at:      0     580     819 
   Size:        580     239
 
SacI G_AGCT'C

Cuts at:      0     614     819 
   Size:        614     205
 
ScaI AGT'ACT

Cuts at:      0      46     819 
   Size:         46     773
 
SexAI A'CCwGG_T

Cuts at:      0     107     620     819 
   Size:        107     513     199

  Fragments arranged by size: 

                 513     199     107
 
SfcI C'TryA_G

Cuts at:      0     235     396     819 
   Size:        235     161     423

  Fragments arranged by size: 

                 423     235     161
 
SfoI GGC'GCC

Cuts at:      0     343     819 
   Size:        343     476
 
SmlI C'TyrA_G

Cuts at:      0     665     819 
   Size:        665     154
 
StyI C'CwwG_G

Cuts at:      0     324     819 
   Size:        324     495
 
TatI w'GTAC_w

Cuts at:      0      44     819 
   Size:         44     775
 
XmnI GAAnn'nnTTC

Cuts at:      0     553     819 
   Size:        553     266
 

 Enzymes that do cut:

   Acc65I     ApaI    ApaLI     AvaI     BaeI     BanI    BanII     BbeI
     BbsI     BglI Bme1580I     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BsiEI  BsiHKAI Bsp1286I    BssSI   BstAPI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI     EagI
     EarI  EcoICRI  Eco57MI EcoO109I    HaeII   HincII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NspI
     PmlI    PshAI   PspOMI     PstI    PvuII     SacI     ScaI    SexAI
     SfcI     SfoI     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApoI     AscI
     AseI    AsiSI    AvrII    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BsaBI    BsaWI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI
  BstZ17I   Bsu36I     ClaI     DraI   DraIII     DrdI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII
     HpaI     MfeI     MluI     NaeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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