Restriction map for
pF1KB7092
(Circular) (Six-base) MAPSORT of: pF1KB7092.seq Check: 6126 from: 1 to: 3665
pF1KB7092 3665 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 141 141
Size: 3665
AccI GT'mk_AC
Cuts at: 703 703
Size: 3665
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 463 1490 1922 2669 2937 166
Size: 297 1027 432 747 268 894
Fragments arranged by size:
1027 894 747 432 297 268
AflII C'TTAA_G
Cuts at: 121 121
Size: 3665
AflIII A'CryG_T
Cuts at: 516 1017 2121 3098 516
Size: 501 1104 977 1083
Fragments arranged by size:
1104 1083 977 501
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AleI CACnn'nnGTG
Cuts at: 586 586
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 141 970 2537 141
Size: 829 1567 1269
Fragments arranged by size:
1567 1269 829
ApaI G_GGCC'C
Cuts at: 526 526
Size: 3665
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 669 3018 3274 669
Size: 2349 256 1060
Fragments arranged by size:
2349 1060 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 685 685
Size: 3665
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 287 372 681 1095 1343 1378 287
Size: 85 309 414 248 35 2574
Fragments arranged by size:
2574 414 309 248 85 35
BanII G_rGCy'C
Cuts at: 398 526 679 1709 398
Size: 128 153 1030 2354
Fragments arranged by size:
2354 1030 153 128
BbeI G_GCGC'C
Cuts at: 291 1099 1347 291
Size: 808 248 2609
Fragments arranged by size:
2609 808 248
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 259 777 259
Size: 518 3147
Bme1580I G_kGCm'C
Cuts at: 377 512 526 1290 1383 2439 377
Size: 135 14 764 93 1056 1603
Fragments arranged by size:
1603 1056 764 135 93 14
BmgBI CAC'GTC
Cuts at: 531 531
Size: 3665
BmrI ACTGGGnnnn_n'
Cuts at: 177 877 958 1293 3437 177
Size: 700 81 335 2144 405
Fragments arranged by size:
2144 700 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 841 1720 2212 2510 439
Size: 402 879 492 298 1594
Fragments arranged by size:
1594 879 492 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 513 615 1018 513
Size: 102 403 3160
Fragments arranged by size:
3160 403 102
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 288 1096 1344 2046 3542 288
Size: 808 248 702 1496 411
Fragments arranged by size:
1496 808 702 411 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 646 442
Size: 204 3461
BseYI C'CCAG_C
Cuts at: 1090 1848 2425 2921 1090
Size: 758 577 496 1834
Fragments arranged by size:
1834 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 3665
BsiEI CG_ry'CG
Cuts at: 104 338 1207 1253 2461 104
Size: 234 869 46 1208 1308
Fragments arranged by size:
1308 1208 869 234 46
BsiHKAI G_wGCw'C
Cuts at: 398 452 679 1457 1647 2439 398
Size: 54 227 778 190 792 1624
Fragments arranged by size:
1624 792 778 227 190 54
BsiWI C'GTAC_G
Cuts at: 513 513
Size: 3665
Bsp1286I G_dGCh'C
Cuts at: 377 398 452 512 526 679 1290 1383 1457
Size: 21 54 60 14 153 611 93 74
Cuts at: 1457 1647 1709 2439 377
Size: 190 62 730 1603
Fragments arranged by size:
1603 730 611 190 153 93 74 62
60 54 21 14
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 197 308 715 929 1231 1612 141
Size: 56 111 407 214 302 381 2194
Fragments arranged by size:
2194 407 381 302 214 111 56
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1577 1577
Size: 3665
BsrFI r'CCGG_y
Cuts at: 218 1663 1844 2053 218
Size: 1445 181 209 1830
Fragments arranged by size:
1830 1445 209 181
BssHII G'CGCG_C
Cuts at: 327 327
Size: 3665
BssSI C'ACGA_G
Cuts at: 130 193 1936 2294 130
Size: 63 1743 358 1501
Fragments arranged by size:
1743 1501 358 63
BstAPI GCAn_nnn'nTGC
Cuts at: 141 198 1042 141
Size: 57 844 2764
Fragments arranged by size:
2764 844 57
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstEII G'GTnAC_C
Cuts at: 174 534 174
Size: 360 3305
BstYI r'GATC_y
Cuts at: 1 690 1157 1180 1515 1761 2773 2859 2871
Size: 689 467 23 335 246 1012 86 12
Cuts at: 2871 3217 1
Size: 346 449
Fragments arranged by size:
1012 689 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 488 107
Size: 381 3284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 653 1615 1796 1959 653
Size: 962 181 163 2359
Fragments arranged by size:
2359 962 181 163
BtsI GCAGTG_nn'
Cuts at: 908 908
Size: 3665
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 335 1250 1424 1815 1842 3187 335
Size: 915 174 391 27 1345 813
Fragments arranged by size:
1345 915 813 391 174 27
EagI C'GGCC_G
Cuts at: 335 1250 335
Size: 915 2750
EarI CTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2183 2329 3475 1873
Size: 310 146 1146 2063
Fragments arranged by size:
2063 1146 310 146
EcoICRI GAG'CTC
Cuts at: 396 677 396
Size: 281 3384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 412 463 1490 1922 2669 2937 166
Size: 246 51 1027 432 747 268 894
Fragments arranged by size:
1027 894 747 432 268 246 51
EcoO109I rG'GnC_Cy
Cuts at: 523 804 523
Size: 281 3384
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 463 1446 463
Size: 983 2682
HaeII r_GCGC'y
Cuts at: 291 1099 1347 2369 3348 291
Size: 808 248 1022 979 608
Fragments arranged by size:
1022 979 808 608 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1507 1537 1539 1569 1507
Size: 30 2 30 3603
Fragments arranged by size:
3603 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
HindIII A'AGCT_T
Cuts at: 118 118
Size: 3665
KasI G'GCGC_C
Cuts at: 287 1095 1343 287
Size: 808 248 2609
Fragments arranged by size:
2609 808 248
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 516 3098 516
Size: 2582 1083
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2520 2336
Size: 184 3481
MscI TGG'CCA
Cuts at: 1426 1426
Size: 3665
MslI CAynn'nnrTG
Cuts at: 586 1654 1781 586
Size: 1068 127 2470
Fragments arranged by size:
2470 1068 127
MspA1I CmG'CkG
Cuts at: 228 258 776 1090 1450 2463 2708 228
Size: 30 518 314 360 1013 245 1185
Fragments arranged by size:
1185 1013 518 360 314 245 30
NaeI GCC'GGC
Cuts at: 1846 1846
Size: 3665
NarI GG'CG_CC
Cuts at: 288 1096 1344 288
Size: 808 248 2609
Fragments arranged by size:
2609 808 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3665
NgoMIV G'CCGG_C
Cuts at: 1844 1844
Size: 3665
NspI r_CATG'y
Cuts at: 718 1218 2125 718
Size: 500 907 2258
Fragments arranged by size:
2258 907 500
PciI A'CATG_T
Cuts at: 2121 2121
Size: 3665
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PmlI CAC'GTG
Cuts at: 615 615
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PspOMI G'GGCC_C
Cuts at: 522 522
Size: 3665
PstI C_TGCA'G
Cuts at: 227 712 1397 227
Size: 485 685 2495
Fragments arranged by size:
2495 685 485
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 258 1090 1450 258
Size: 832 360 2473
Fragments arranged by size:
2473 832 360
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 398 679 398
Size: 281 3384
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 223 708 1393 2386 2577 33
Size: 190 485 685 993 191 1121
Fragments arranged by size:
1121 993 685 485 191 190
SfoI GGC'GCC
Cuts at: 289 1097 1345 289
Size: 808 248 2609
Fragments arranged by size:
2609 808 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 121 454 820 1735 2227 2489 121
Size: 333 366 915 492 262 1297
Fragments arranged by size:
1297 915 492 366 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
StyI C'CwwG_G
Cuts at: 107 799 1153 107
Size: 692 354 2619
Fragments arranged by size:
2619 692 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 576 1248 1381 1840 2049 576
Size: 672 133 459 209 2192
Fragments arranged by size:
2192 672 459 209 133
TatI w'GTAC_w
Cuts at: 623 3180 623
Size: 2557 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 3633
Tth111I GACn'n_nGTC
Cuts at: 406 577 1462 406
Size: 171 885 2609
Fragments arranged by size:
2609 885 171
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1160 1160
Size: 3665
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BsmI BsmBI BsrGI BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI