Restriction map for pF1KB7091
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbsI        BbvCI       
BcgI        BciVI       BfrI        BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MseI        MspA1I      MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          319  GTAGAC               GT^[AC][GT]AC
AccII         172  CGCG                 CG^CG
AfaI          144  GTAC                 GT^AC
AflIII        434  ACGTGT               A^C[GA][CT]GT
AlwNI         274  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         121  GAGCTC               G[AT]GC[AT]^C
AvaII         183  GGACC                G^G[AT]CC
BalI          197  TGGCCA               TGG^CCA
BanI          289  GGTGCC               G^G[CT][GA]CC
BanII         121  GAGCTC               G[GA]GC[CT]^C
BbrPI         433  CACGTG               CAC^GTG
BcnI          369  CCGGG                CC^[GC]GG
BglI          292  GCCATGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         509  AGATCT               A^GATCT
BmyI          121  GAGCTC               G[AGT]GC[ACT]^C
BsaAI         433  CACGTG               [CT]AC^GT[GA]
BsgI          217  CTGCAC               GTGCAG(16/14)
BsiHKAI       121  GAGCTC               G[AT]GC[AT]^C
BsmAI          97  GAGAC                GTCTC(1/5)
Bsp1286I      121  GAGCTC               G[AGT]GC[ACT]^C
BsrDI         224  GCAATG               GCAATG(2/0)
BsrFI         155  GCCGGC               [GA]^CCGG[CT]
BsrI          242  CCAGT                ACTGG(1/-1)
BstUI         172  CGCG                 CG^CG
BstYI         509  AGATCT               [GA]^GATC[CT]
CfoI          171  GCGC                 GCG^C
Cfr10I        155  GCCGGC               [GA]^CCGG[CT]
DraII         182  AGGACCT              [GA]G^G[ACGT]CC[CT]
EarI           85  CTCTTC               CTCTTC(1/4)
EcoO109I      182  AGGACCT              [GA]G^G[ACGT]CC[CT]
HhaI          171  GCGC                 GCG^C
HinP1I        171  GCGC                 G^CGC
Ksp632I        85  CTCTTC               CTCTTC(1/4)
MaeII         434  ACGT                 A^CGT
MflI          509  AGATCT               [GA]^GATC[CT]
MluNI         197  TGGCCA               TGG^CCA
MscI          197  TGGCCA               TGG^CCA
MvnI          172  CGCG                 CG^CG
NaeI          155  GCCGGC               GCC^GGC
NciI          369  CCGGG                CC^[GC]GG
NgoMIV        155  GCCGGC               G^CCGGC
PmaCI         433  CACGTG               CAC^GTG
PmlI          433  CACGTG               CAC^GTG
PpuMI         182  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         454  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          144  GTAC                 GT^AC
SacI          121  GAGCTC               GAGCT^C
SfaNI         425  GCATC                GCATC(5/9)
TaqI          332  TCGA                 T^CGA
TthHB8I       332  TCGA                 T^CGA
XhoII         509  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BstF5I        426  CATCC                GGATG(2/0)
              372  GGATG               
BstXI         200  CCATCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              242  CCAGTGAGCTGG        
EaeI          197  TGGCCA               [CT]^GGCC[GA]
              420  TGGCCG              
EcoT14I       137  CCAAGG               C^C[AT][AT]GG
              501  CCTTGG              
FauI          263  CCCGC                CCCGC(4/6)
               64  GCGGG               
FokI          426  CATCC                GGATG(9/13)
              372  GGATG               
HapII         156  CCGG                 C^CGG
              369  CCGG                
HinfI           4  GAGTC                G^A[ACGT]TC
              521  GACTC               
HpaII         156  CCGG                 C^CGG
              369  CCGG                
HphI          313  GGTGA                GGTGA(8/7)
              326  TCACC               
MaeIII        414  GTCAC                ^GT[ACGT]AC
              515  GTGAC               
MboII          42  GAAGA                GAAGA(8/7)
               86  TCTTC               
MslI          201  CATCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              220  CACCGCAATG          
MspI          156  CCGG                 C^CGG
              369  CCGG                
NlaIII        294  CATG                 CATG^
              418  CATG                
PleI          521  GACTC                GAGTC(4/5)
                4  GAGTC               
StyI          137  CCAAGG               C^C[AT][AT]GG
              501  CCTTGG              
Tsp45I        414  GTCAC                ^GT[GC]AC
              515  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AciI        AluI        BbvI        BclI        BfaI        
BsaJI       BsiYI       BslI        BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
FbaI        Fnu4HI      HaeIII      ItaI        MaeI        
MboI        MnlI        MvaI        MwoI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       TseI        
TspRI       
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