Restriction map for pF1KB7089
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      Cfr13I      CpoI        
DraI        DraII       DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HapII       HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        Sau96I      
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     TspRI       Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcyI          373  GACGCC               G[GA]^CG[CT]C
AflIII        200  ACGTGT               A^C[GA][CT]GT
AspHI          88  GTGCTC               G[AT]GC[AT]^C
BcgI          321  GCAGGACTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          417  GCCCTCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          420  CTCCAG               CTGGAG(16/14)
BsaAI         199  TACGTG               [CT]AC^GT[GA]
BsaHI         373  GACGCC               G[GA]^CG[CT]C
BsiHKAI        88  GTGCTC               G[AT]GC[AT]^C
BspDI         295  ATCGAT               AT^CGAT
BstXI         278  CCATCGCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          295  ATCGAT               AT^CGAT
DdeI          106  CTGAG                C^T[ACGT]AG
DpnII         237  GATC                 ^GATC
EagI          389  CGGCCG               C^GGCCG
EarI           58  CTCTTC               CTCTTC(1/4)
EclXI         389  CGGCCG               C^GGCCG
Eco52I        389  CGGCCG               C^GGCCG
EcoT14I       140  CCAAGG               C^C[AT][AT]GG
HaeII         288  GGCGCT               [GA]GCGC^[CT]
HgaI          373  GACGC                GACGC(5/10)
Hin1I         373  GACGCC               G[GA]^CG[CT]C
Ksp632I        58  CTCTTC               CTCTTC(1/4)
KspI          386  CCGCGG               CCGC^GG
MaeII         200  ACGT                 A^CGT
MaeIII        217  GTCAC                ^GT[ACGT]AC
MboI          237  GATC                 ^GATC
NdeII         237  GATC                 ^GATC
NlaIV         414  GGAGCC               GG[ACGT]^[ACGT]CC
NotI          388  GCGGCCGC             GC^GGCCGC
PstI          319  CTGCAG               CTGCA^G
SacII         386  CCGCGG               CCGC^GG
Sau3AI        237  GATC                 ^GATC
SbfI          318  CCTGCAGG             CCTGCA^GG
SfcI          319  CTGCAG               C^T[GA][CT]AG
SmlI          166  CTCAAG               C^T[CT][GA]AG
Sse8387I      318  CCTGCAGG             CCTGCA^GG
StyI          140  CCAAGG               C^C[AT][AT]GG
Tsp45I        217  GTCAC                ^GT[GC]AC
XcmI          161  CCATCCTCAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          198  GTAC                 GT^AC
              204  GTAC                
BanII         406  GAGCCC               G[GA]GC[CT]^C
              415  GAGCCC              
BsiEI          99  CGACCG               CG[GA][CT]^CG
              389  CGGCCG              
BssHII        131  GCGCGC               G^CGCGC
              133  GCGCGC              
BssKI         339  CCAGG                ^CC[ACGT]GG
              422  CCAGG               
BstF5I        162  CATCC                GGATG(2/0)
              428  CATCC               
BstNI         339  CCAGG                CC^[AT]GG
              422  CCAGG               
DsaI          377  CCGTGG               C^C[GA][CT]GG
              386  CCGCGG              
EaeI          275  CGGCCA               [CT]^GGCC[GA]
              389  CGGCCG              
EcoRII        339  CCAGG                ^CC[AT]GG
              422  CCAGG               
FokI          162  CATCC                GGATG(9/13)
              428  CATCC               
MspA1I        246  CCGCTG               C[AC]G^C[GT]G
              386  CCGCGG              
MvaI          339  CCAGG                CC^[AT]GG
              422  CCAGG               
RsaI          198  GTAC                 GT^AC
              204  GTAC                
ScrFI         339  CCAGG                CC^[ACGT]GG
              422  CCAGG               
TaqI          239  TCGA                 T^CGA
              296  TCGA                
TthHB8I       239  TCGA                 T^CGA
              296  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BstUI       
Cac8I       CfoI        FauI        Fnu4HI      HaeIII      
HhaI        HinP1I      HphI        ItaI        MboII       
MnlI        MvnI        MwoI        TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com