Restriction map for pF1KB7055
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BcnI        BfrI        BglI        BglII       BlpI        
BpmI        Bpu1102I    BsaBI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsoBI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        HinP1I      KasI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        1247  GACGTC               GACGT^C
AccI          211  GTAGAC               GT^[AC][GT]AC
AciI          101  GCGG                 CCGC(-3/-1)
AcyI         1247  GACGTC               G[GA]^CG[CT]C
Alw44I        539  GTGCAC               G^TGCAC
AlwNI         530  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         539  GTGCAC               G^TGCAC
AvrII         297  CCTAGG               C^CTAGG
BamHI         565  GGATCC               G^GATCC
BanII         775  GAGCTC               G[GA]GC[CT]^C
BlnI          297  CCTAGG               C^CTAGG
BsaAI         872  CACGTA               [CT]AC^GT[GA]
BsaHI        1247  GACGTC               G[GA]^CG[CT]C
BsaI          341  GAGACC               GGTCTC(1/5)
BsgI          719  CTGCAC               GTGCAG(16/14)
BsmBI        1249  CGTCTC               CGTCTC(1/5)
BsmI          261  GAATGC               GAATGC(1/-1)
DraIII        557  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          897  CCATGG               C^C[GA][CT]GG
EcoNI         267  CCTACATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1172  GAATTC               G^AATTC
HapII         840  CCGG                 C^CGG
Hin1I        1247  GACGTC               G[GA]^CG[CT]C
HindIII       524  AAGCTT               A^AGCTT
HpaI          607  GTTAAC               GTT^AAC
HpaII         840  CCGG                 C^CGG
HphI          894  TCACC                GGTGA(8/7)
MfeI           91  CAATTG               C^AATTG
MseI          608  TTAA                 T^TAA
MslI          295  CACCTAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         56  CAGCTG               C[AC]G^C[GT]G
MspI          840  CCGG                 C^CGG
MunI           91  CAATTG               C^AATTG
MwoI          532  GCCTCTGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          897  CCATGG               C^CATGG
PflMI         660  CCAAAATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         1589  CTGCAG               CTGCA^G
PvuII          56  CAGCTG               CAG^CTG
SacI          775  GAGCTC               GAGCT^C
SapI         1135  GAAGAGC              GCTCTTC(1/4)
SfaNI        1091  GCATC                GCATC(5/9)
SfcI         1589  CTGCAG               C^T[GA][CT]AG
SpeI          600  ACTAGT               A^CTAGT
SspI         1663  AATATT               AAT^ATT
Tru9I         608  TTAA                 T^TAA
Tsp45I       1656  GTCAC                ^GT[GC]AC
Van91I        660  CCAAAATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI          633  AAATTC               [GA]^AATT[CT]
             1172  GAATTC              
ApoI          633  AAATTC               [GA]^AATT[CT]
             1172  GAATTC              
AvaII         233  GGACC                G^G[AT]CC
              745  GGACC               
BbsI         1723  GAAGAC               GAAGAC(2/6)
              867  GTCTTC              
BbvI           55  GCAGC                GCAGC(8/12)
              935  GCAGC               
BclI          222  TGATCA               T^GATCA
             1698  TGATCA              
BpuAI        1723  GAAGAC               GAAGAC(2/6)
              867  GTCTTC              
BsaJI         297  CCTAGG               C^C[ACGT][ACGT]GG
              897  CCATGG              
BsmFI          66  GGGAC                GGGAC(10/14)
              412  GGGAC               
Bsp1407I      912  TGTACA               T^GTACA
             1272  TGTACA              
BspHI         225  TCATGA               T^CATGA
             1617  TCATGA              
BsrGI         912  TGTACA               T^GTACA
             1272  TGTACA              
BstYI         103  GGATCT               [GA]^GATC[CT]
              565  GGATCC              
EcoT14I       297  CCTAGG               C^C[AT][AT]GG
              897  CCATGG              
FbaI          222  TGATCA               T^GATCA
             1698  TGATCA              
Fnu4HI         55  GCAGC                GC^[ACGT]GC
              935  GCAGC               
ItaI           55  GCAGC                GC^[ACGT]GC
              935  GCAGC               
MflI          103  GGATCT               [GA]^GATC[CT]
              565  GGATCC              
RcaI          225  TCATGA               T^CATGA
             1617  TCATGA              
SmlI          428  CTCAAG               C^T[CT][GA]AG
             1187  CTCAAG              
SspBI         912  TGTACA               T^GTACA
             1272  TGTACA              
StyI          297  CCTAGG               C^C[AT][AT]GG
              897  CCATGG              
TaqI         1123  TCGA                 T^CGA
             1231  TCGA                
TfiI          795  GAATC                G^A[AT]TC
             1645  GAATC               
TseI           55  GCAGC                G^C[AT]GC
              935  GCAGC               
TspRI        1257  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1650  CAGTG               
TthHB8I      1123  TCGA                 T^CGA
             1231  TCGA                
XhoII         103  GGATCT               [GA]^GATC[CT]
              565  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AfaI        AluI        AlwI        AspHI       BalI        
BciVI       BfaI        BmyI        BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EarI        EcoRII      FokI        
HaeIII      HincII      HindII      HinfI       Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MvaI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       Tsp509I     XcmI        
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