Restriction map for pF1KB7032
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AspHI       AspI        AvrII       BamHI       
BanII       BbsI        BcgI        BciVI       BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrFI       BsrGI       
BssSI       Bst1107I    BstBI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRI       EcoRV       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   70 enzymes) #
###################################

Acc65I       1218  GGTACC               G^GTACC
AcyI          206  GGCGCC               G[GA]^CG[CT]C
AfaI         1219  GTAC                 GT^AC
AlwI          951  GATCC                GGATC(4/5)
AlwNI         842  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          642  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1163  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1218  GGTACC               G^GTACC
AvaI         1064  CCCGAG               C^[CT]CG[GA]G
AvaII         195  GGACC                G^G[AT]CC
BbeI          206  GGCGCC               GGCGC^C
BbrPI         805  CACGTG               CAC^GTG
BbvCI         943  GCTGAGG              CCTCAGC(-5/-2)
BcnI           24  CCCGG                CC^[GC]GG
BmyI         1061  GTGCCC               G[AGT]GC[ACT]^C
BsaAI         805  CACGTG               [CT]AC^GT[GA]
BsaHI         206  GGCGCC               G[GA]^CG[CT]C
BsaI           83  GAGACC               GGTCTC(1/5)
BsgI         1056  GTGCAG               GTGCAG(16/14)
BsmI          451  GAATGC               GAATGC(1/-1)
BsoBI        1064  CCCGAG               C^[CT]CG[GA]G
Bsp1286I     1061  GTGCCC               G[AGT]GC[ACT]^C
BspHI         533  TCATGA               T^CATGA
BspMI          62  ACCTGC               ACCTGC(4/8)
BsrBI         117  GAGCGG               CCGCTC(-3/-3)
BsrDI         939  CATTGC               GCAATG(2/0)
BssHII         43  GCGCGC               G^CGCGC
BstAPI        611  GCAAGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        637  GGTGACC              G^GT[ACGT]ACC
BstPI         637  GGTGACC              G^GT[ACGT]ACC
BstXI         185  CCACCTTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         950  AGATCC               [GA]^GATC[CT]
Bsu36I        642  CCTGAGG              CC^T[ACGT]AGG
DraII         121  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        351  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          865  GACTTGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        642  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      121  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       637  GGTGACC              G^GT[ACGT]ACC
HaeII         206  GGCGCC               [GA]GCGC^[CT]
HapII          25  CCGG                 C^CGG
HgaI          161  GACGC                GACGC(5/10)
Hin1I         206  GGCGCC               G[GA]^CG[CT]C
HpaII          25  CCGG                 C^CGG
KasI          206  GGCGCC               G^GCGCC
KpnI         1218  GGTACC               GGTAC^C
MaeII         806  ACGT                 A^CGT
MflI          950  AGATCC               [GA]^GATC[CT]
MseI         1037  TTAA                 T^TAA
MspA1I        246  CAGCTG               C[AC]G^C[GT]G
MspI           25  CCGG                 C^CGG
NarI          206  GGCGCC               GG^CGCC
NciI           24  CCCGG                CC^[GC]GG
NcoI          291  CCATGG               C^CATGG
PmaCI         805  CACGTG               CAC^GTG
PmlI          805  CACGTG               CAC^GTG
PshAI         363  GACTAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          243  CTGCAG               CTGCA^G
PvuII         246  CAGCTG               CAG^CTG
RcaI          533  TCATGA               T^CATGA
RsaI         1219  GTAC                 GT^AC
SapI          255  GCTCTTC              GCTCTTC(1/4)
SfoI          206  GGCGCC               GGC^GCC
SspI          487  AATATT               AAT^ATT
TaqI          761  TCGA                 T^CGA
Tru9I        1037  TTAA                 T^TAA
TthHB8I       761  TCGA                 T^CGA
XcmI          857  CCAACCAAGACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         950  AGATCC               [GA]^GATC[CT]
XmnI         1163  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII          44  CGCG                 CG^CG
               74  CGCG                
AcsI         1021  AAATTT               [GA]^AATT[CT]
             1131  AAATTC              
ApoI         1021  AAATTT               [GA]^AATT[CT]
             1131  AAATTC              
AviII          65  TGCGCA               TGC^GCA
              674  TGCGCA              
BanI          206  GGCGCC               G^G[CT][GA]CC
             1218  GGTACC              
BpmI          481  CTCCAG               CTGGAG(16/14)
             1241  CTCCAG              
BsmAI          83  GAGAC                GTCTC(1/5)
             1122  GTCTC               
BsmFI         296  GGGAC                GGGAC(10/14)
              824  GTCCC               
BsrI          735  ACTGG                ACTGG(1/-1)
              907  ACTGG               
BstUI          44  CGCG                 CG^CG
               74  CGCG                
DsaI          291  CCATGG               C^C[GA][CT]GG
             1051  CCACGG              
EarI          256  CTCTTC               CTCTTC(1/4)
              394  CTCTTC              
EcoT14I       188  CCTTGG               C^C[AT][AT]GG
              291  CCATGG              
FauI          101  CCCGC                CCCGC(4/6)
              119  GCGGG               
FspI           65  TGCGCA               TGC^GCA
              674  TGCGCA              
Ksp632I       256  CTCTTC               CTCTTC(1/4)
              394  CTCTTC              
MvnI           44  CGCG                 CG^CG
               74  CGCG                
PflMI         210  CCACACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              861  CCAAGACTTGG         
PleI         1239  GACTC                GAGTC(4/5)
              346  GAGTC               
SfcI          169  CTACAG               C^T[GA][CT]AG
              243  CTGCAG              
StyI          188  CCTTGG               C^C[AT][AT]GG
              291  CCATGG              
TfiI          477  GATTC                G^A[AT]TC
             1267  GAATC               
Van91I        210  CCACACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              861  CCAAGACTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        BalI        BbvI        BclI        
BsaJI       BseRI       BsiYI       BslI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EcoRII      FbaI        
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      Tsp509I     TspRI       
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