Restriction map for pF1KB5677
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BciVI       BfaI        
BfrI        BglI        BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaJI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstNI       BstUI       BstXI       
BstZ17I     Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoRII      
EcoT14I     EcoT22I     FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        KasI        KspI        MaeI        
MfeI        MluI        MluNI       MscI        MspA1I      
MunI        MvaI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        250  GGTACC               G^GTACC
AccIII         96  TCCGGA               T^CCGGA
AocI          115  CCTCAGG              CC^T[ACGT]AGG
AseI          799  ATTAAT               AT^TAAT
AsnI          799  ATTAAT               AT^TAAT
Asp718I       250  GGTACC               G^GTACC
AvaII         311  GGTCC                G^G[AT]CC
BbsI          338  GAAGAC               GAAGAC(2/6)
BcgI          276  CGATCCTCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          192  TGATCA               T^GATCA
BcnI          859  CCCGG                CC^[GC]GG
BglII         580  AGATCT               A^GATCT
BpmI          473  CTCCAG               CTGGAG(16/14)
BpuAI         338  GAAGAC               GAAGAC(2/6)
BsaBI         382  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI          96  TCCGGA               T^CCGGA
BsmAI         431  GAGAC                GTCTC(1/5)
BspEI          96  TCCGGA               T^CCGGA
BspHI         483  TCATGA               T^CATGA
BssKI         859  CCCGG                ^CC[ACGT]GG
BstEII        922  GGTGACC              G^GT[ACGT]ACC
BstPI         922  GGTGACC              G^GT[ACGT]ACC
Bsu36I        115  CCTCAGG              CC^T[ACGT]AGG
EarI          505  GAAGAG               CTCTTC(1/4)
Eco81I        115  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       922  GGTGACC              G^GT[ACGT]ACC
EcoRI         438  GAATTC               G^AATTC
EcoRV         422  GATATC               GAT^ATC
FauI          455  CCCGC                CCCGC(4/6)
FbaI          192  TGATCA               T^GATCA
HaeIII        449  GGCC                 GG^CC
HindIII       149  AAGCTT               A^AGCTT
HinfI         361  GATTC                G^A[ACGT]TC
KpnI          250  GGTACC               GGTAC^C
Ksp632I       505  GAAGAG               CTCTTC(1/4)
MaeII         918  ACGT                 A^CGT
MamI          382  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI           96  TCCGGA               T^CCGGA
MslI          828  CATACCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          286  GCTACATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          859  CCCGG                CC^[GC]GG
PshBI         799  ATTAAT               AT^TAAT
RcaI          483  TCATGA               T^CATGA
ScaI          984  AGTACT               AGT^ACT
ScrFI         859  CCCGG                CC^[ACGT]GG
TaqI          546  TCGA                 T^CGA
TfiI          361  GATTC                G^A[AT]TC
Tsp45I        923  GTGAC                ^GT[GC]AC
TspRI         812  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       546  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI          456  CCGC                 CCGC(-3/-1)
              621  GCGG                
AluI           42  AGCT                 AG^CT
              150  AGCT                
BanI          250  GGTACC               G^G[CT][GA]CC
              631  GGCACC              
BsaWI          96  TCCGGA               [AT]^CCGG[AT]
              257  TCCGGT              
BsiYI         649  CCAAGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              860  CCGGAATGAGG         
BslI          649  CCAAGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              860  CCGGAATGAGG         
BsrI          813  ACTGG                ACTGG(1/-1)
              475  CCAGT               
BstYI         468  AGATCC               [GA]^GATC[CT]
              580  AGATCT              
Cfr13I        311  GGTCC                G^G[ACGT]CC
              448  GGGCC               
DraI          133  TTTAAA               TTT^AAA
              727  TTTAAA              
HphI          385  GGTGA                GGTGA(8/7)
              922  GGTGA               
MaeIII        575  GTTAC                ^GT[ACGT]AC
              923  GTGAC               
MflI          468  AGATCC               [GA]^GATC[CT]
              580  AGATCT              
Sau96I        311  GGTCC                G^G[ACGT]CC
              448  GGGCC               
XhoII         468  AGATCC               [GA]^GATC[CT]
              580  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AcsI        AfaI        AlwI        ApoI        BbvI        
BseRI       BstF5I      DdeI        DpnII       Fnu4HI      
FokI        HapII       HpaII       ItaI        MboI        
MboII       MnlI        MseI        MspI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Tru9I       
TseI        Tsp509I     
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