Restriction map for pF1KB5582
 (Linear) (Six-base) MAPSORT of: KIBB5582.seq  Check: 180  from: 1  to: 1122



KIBB5582.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

Acc65I G'GTAC_C

Cuts at:      0     376    1122 
   Size:        376     746
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     872    1122 
   Size:        268     604     250

  Fragments arranged by size: 

                 604     268     250
 
AgeI A'CCGG_T

Cuts at:      0     532    1122 
   Size:        532     590
 
AleI CACnn'nnGTG

Cuts at:      0     451    1122 
   Size:        451     671
 
ApoI r'AATT_y

Cuts at:      0     301     682    1030    1122 
   Size:        301     381     348      92

  Fragments arranged by size: 

                 381     348     301      92
 
AseI AT'TA_AT

Cuts at:      0     364    1122 
   Size:        364     758
 
BanI G'GyrC_C

Cuts at:      0      81     376    1122 
   Size:         81     295     746

  Fragments arranged by size: 

                 746     295      81
 
BfrBI ATG'CAT

Cuts at:      0    1086    1122 
   Size:       1086      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     591    1122 
   Size:        591     531
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     856    1122 
   Size:        335     521     266

  Fragments arranged by size: 

                 521     335     266
 
BsaHI Gr'CG_yC

Cuts at:      0     860    1122 
   Size:        860     262
 
BsaWI w'CCGG_w

Cuts at:      0     532    1122 
   Size:        532     590
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82     617     794    1122 
   Size:         82     535     177     328

  Fragments arranged by size: 

                 535     328     177      82
 
BseYI C'CCAG_C

Cuts at:      0     854    1122 
   Size:        854     268
 
BsiHKAI G_wGCw'C

Cuts at:      0     761    1122 
   Size:        761     361
 
BsmI GAATG_Cn'

Cuts at:      0     301    1086    1100    1122 
   Size:        301     785      14      22

  Fragments arranged by size: 

                 785     301      22      14
 
Bsp1286I G_dGCh'C

Cuts at:      0     761    1122 
   Size:        761     361
 
BspHI T'CATG_A

Cuts at:      0      96     821    1122 
   Size:         96     725     301

  Fragments arranged by size: 

                 725     301      96
 
BsrDI GCAATG_nn'

Cuts at:      0     116    1122 
   Size:        116    1006
 
BsrFI r'CCGG_y

Cuts at:      0     532    1122 
   Size:        532     590
 
BstBI TT'CG_AA

Cuts at:      0     105    1104    1122 
   Size:        105     999      18

  Fragments arranged by size: 

                 999     105      18
 
BstYI r'GATC_y

Cuts at:      0     480    1122 
   Size:        480     642
 
Bsu36I CC'TnA_GG

Cuts at:      0     170     728    1122 
   Size:        170     558     394

  Fragments arranged by size: 

                 558     394     170
 
BtsI GCAGTG_nn'

Cuts at:      0     122     554     947    1122 
   Size:        122     432     393     175

  Fragments arranged by size: 

                 432     393     175     122
 
DraIII CAC_nnn'GTG

Cuts at:      0     289    1122 
   Size:        289     833
 
EarI CTCTTCn'nnn_

Cuts at:      0     961    1122 
   Size:        961     161
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     872    1122 
   Size:        268     604     250

  Fragments arranged by size: 

                 604     268     250
 
EcoRV GAT'ATC

Cuts at:      0     462    1122 
   Size:        462     660
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     526    1122 
   Size:        526     596
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1029    1061    1122 
   Size:       1029      32      61

  Fragments arranged by size: 

                1029      61      32
 
KpnI G_GTAC'C

Cuts at:      0     380    1122 
   Size:        380     742
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     588     855    1122 
   Size:        545      43     267     267

  Fragments arranged by size: 

                 545     267     267      43
 
MslI CAynn'nnrTG

Cuts at:      0     140     203     451     661     766    1122 
   Size:        140      63     248     210     105     356

  Fragments arranged by size: 

                 356     248     210     140     105      63
 
MspA1I CmG'CkG

Cuts at:      0     921    1122 
   Size:        921     201
 
NsiI A_TGCA'T

Cuts at:      0    1088    1122 
   Size:       1088      34
 
NspI r_CATG'y

Cuts at:      0     845    1122 
   Size:        845     277
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     289    1122 
   Size:        289     833
 
PfoI T'CCnGG_A

Cuts at:      0     649     749    1122 
   Size:        649     100     373

  Fragments arranged by size: 

                 649     373     100
 
PstI C_TGCA'G

Cuts at:      0      33    1122 
   Size:         33    1089
 
PvuII CAG'CTG

Cuts at:      0     921    1122 
   Size:        921     201
 
SfcI C'TryA_G

Cuts at:      0      29    1122 
   Size:         29    1093
 
SmlI C'TyrA_G

Cuts at:      0     350     835    1122 
   Size:        350     485     287

  Fragments arranged by size: 

                 485     350     287
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     787    1122 
   Size:        787     335
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     263     295     645     677    1122 
   Size:        263      32     350      32     445

  Fragments arranged by size: 

                 445     350     263      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     262    1122 
   Size:        262     860
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AleI     ApoI     AseI     BanI    BfrBI
     BplI    BpuEI    BsaHI    BsaWI    BseRI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrDI    BsrFI    BstBI    BstYI   Bsu36I     BtsI
   DraIII     EarI  Eco57MI    EcoRV     FalI    Hin4I     KpnI     MmeI
     MslI   MspA1I     NsiI     NspI    PflMI     PfoI     PstI    PvuII
     SfcI     SmlI    TaqII     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaXI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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