Restriction map for pF1KB5574
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BanI        BanII       BbeI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoO109I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
MaeI        MamI        MfeI        MflI        MluI        
MroI        MseI        MslI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmeI        PpuMI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          250  GTCTAC               GT^[AC][GT]AC
AfaI          111  GTAC                 GT^AC
Alw44I        115  GTGCAC               G^TGCAC
AlwI          237  GATCC                GGATC(4/5)
AlwNI         542  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         115  GTGCAC               G^TGCAC
AspHI         115  GTGCAC               G[AT]GC[AT]^C
AviII         470  TGCGCA               TGC^GCA
BalI          330  TGGCCA               TGG^CCA
BbrPI         354  CACGTG               CAC^GTG
BbsI           25  GTCTTC               GAAGAC(2/6)
BbvCI         190  GCTGAGG              CCTCAGC(-5/-2)
BmyI          115  GTGCAC               G[AGT]GC[ACT]^C
BpuAI          25  GTCTTC               GAAGAC(2/6)
BseRI          18  CTCCTC               GAGGAG(10/8)
BsiEI         591  CGGCCG               CG[GA][CT]^CG
BsiHKAI       115  GTGCAC               G[AT]GC[AT]^C
BsmAI         498  GAGAC                GTCTC(1/5)
BsmBI         498  GAGACG               CGTCTC(1/5)
Bsp1286I      115  GTGCAC               G[AGT]GC[ACT]^C
BsrI          563  ACTGG                ACTGG(1/-1)
BssSI         445  CTCGTG               CACGAG(-5/-1)
BstEII        303  GGTGACC              G^GT[ACGT]ACC
BstF5I        508  GGATG                GGATG(2/0)
BstPI         303  GGTGACC              G^GT[ACGT]ACC
BstXI          92  CCAAGGCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI          591  CGGCCG               C^GGCCG
EclXI         591  CGGCCG               C^GGCCG
Eco52I        591  CGGCCG               C^GGCCG
EcoNI         186  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       303  GGTGACC              G^GT[ACGT]ACC
FauI          391  GCGGG                CCCGC(4/6)
FokI          508  GGATG                GGATG(9/13)
FspI          470  TGCGCA               TGC^GCA
HphI          303  GGTGA                GGTGA(8/7)
KspI          389  CCGCGG               CCGC^GG
MaeIII        304  GTGAC                ^GT[ACGT]AC
MluNI         330  TGGCCA               TGG^CCA
MscI          330  TGGCCA               TGG^CCA
NaeI          136  GCCGGC               GCC^GGC
NcoI          431  CCATGG               C^CATGG
NgoMIV        136  GCCGGC               G^CCGGC
NlaIV         174  GGTCCC               GG[ACGT]^[ACGT]CC
PflMI         561  CCACTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         354  CACGTG               CAC^GTG
PmlI          354  CACGTG               CAC^GTG
PshAI         513  GACTGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         271  CAGCTG               CAG^CTG
RsaI          111  GTAC                 GT^AC
SacII         389  CCGCGG               CCGC^GG
SmaI          177  CCCGGG               CCC^GGG
SmlI          523  CTCAAG               C^T[CT][GA]AG
Tsp45I        304  GTGAC                ^GT[GC]AC
TspRI         562  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        561  CCACTGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         177  CCCGGG               C^CCGGG
XmaI          177  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI           46  GACGCC               G[GA]^CG[CT]C
              182  GACGCC              
AvaI          157  CCCGAG               C^[CT]CG[GA]G
              177  CCCGGG              
AvaII         174  GGTCC                G^G[AT]CC
              435  GGTCC               
BpmI          147  CTGGAG               CTGGAG(16/14)
              229  CTGGAG              
BsaAI         112  TACGTG               [CT]AC^GT[GA]
              354  CACGTG              
BsaHI          46  GACGCC               G[GA]^CG[CT]C
              182  GACGCC              
BsgI          274  CTGCAC               GTGCAG(16/14)
              575  CTGCAC              
BsoBI         157  CCCGAG               C^[CT]CG[GA]G
              177  CCCGGG              
BsrBI          57  CCGCTC               CCGCTC(-3/-3)
              555  CCGCTC              
BsrFI         136  GCCGGC               [GA]^CCGG[CT]
              171  GCCGGT              
BssHII        132  GCGCGC               G^CGCGC
              152  GCGCGC              
Cfr10I        136  GCCGGC               [GA]^CCGG[CT]
              171  GCCGGT              
DdeI          191  CTGAG                C^T[ACGT]AG
              293  CTGAG               
DpnII          39  GATC                 ^GATC
              237  GATC                
EcoT14I        92  CCAAGG               C^C[AT][AT]GG
              431  CCATGG              
HaeII          14  GGCGCT               [GA]GCGC^[CT]
               96  GGCGCT              
Hin1I          46  GACGCC               G[GA]^CG[CT]C
              182  GACGCC              
MboI           39  GATC                 ^GATC
              237  GATC                
MspA1I        271  CAGCTG               C[AC]G^C[GT]G
              389  CCGCGG              
NdeII          39  GATC                 ^GATC
              237  GATC                
NlaIII         42  CATG                 CATG^
              432  CATG                
Sau3AI         39  GATC                 ^GATC
              237  GATC                
SfaNI         310  GATGC                GCATC(5/9)
              350  GCATC               
StyI           92  CCAAGG               C^C[AT][AT]GG
              431  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BsmFI       BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DsaI        EaeI        EcoRII      Fnu4HI      HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
ItaI        MaeII       MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        PstI        
Sau96I      ScrFI       SfcI        TseI        
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