Restriction map for pF1KB5554
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AlwNI       Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DrdI        EagI        EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
EcoT14I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        Hin1I       HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI           34  GTAGAC               GT^[AC][GT]AC
AciI          840  GCGG                 CCGC(-3/-1)
AcsI          305  GAATTC               [GA]^AATT[CT]
AfaI          472  GTAC                 GT^AC
AflIII        621  ACATGT               A^C[GA][CT]GT
Alw44I        224  GTGCAC               G^TGCAC
AlwI          357  GGATC                GGATC(4/5)
AocI          886  CCTGAGG              CC^T[ACGT]AGG
ApaLI         224  GTGCAC               G^TGCAC
ApoI          305  GAATTC               [GA]^AATT[CT]
BanI          846  GGCACC               G^G[CT][GA]CC
BbvI          679  GCAGC                GCAGC(8/12)
BciVI         147  GTATCC               GTATCC(6/5)
BfaI          418  CTAG                 C^TAG
BpmI          434  CTCCAG               CTGGAG(16/14)
BsaAI         197  TACGTG               [CT]AC^GT[GA]
BsaBI         124  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         212  CCGTGG               C^C[ACGT][ACGT]GG
BsgI          497  GTGCAG               GTGCAG(16/14)
BsmAI           3  GTCTC                GTCTC(1/5)
BspLU11I      621  ACATGT               A^CATGT
BssKI         423  CCAGG                ^CC[ACGT]GG
BstNI         423  CCAGG                CC^[AT]GG
Bsu36I        886  CCTGAGG              CC^T[ACGT]AGG
Cac8I         810  GCCTGC               GC[ACGT]^[ACGT]GC
CfoI          520  GCGC                 GCG^C
DraII         577  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        523  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          212  CCGTGG               C^C[GA][CT]GG
EaeI          209  TGGCCG               [CT]^GGCC[GA]
Eco81I        886  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      577  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         305  GAATTC               G^AATTC
EcoRII        423  CCAGG                ^CC[AT]GG
EcoT22I       601  ATGCAT               ATGCA^T
Fnu4HI        679  GCAGC                GC^[ACGT]GC
HhaI          520  GCGC                 GCG^C
HinP1I        520  GCGC                 G^CGC
HincII        774  GTCAAC               GT[CT]^[GA]AC
HindII        774  GTCAAC               GT[CT]^[GA]AC
HindIII       101  AAGCTT               A^AGCTT
HphI          487  GGTGA                GGTGA(8/7)
ItaI          679  GCAGC                GC^[ACGT]GC
MaeI          418  CTAG                 C^TAG
MaeII         198  ACGT                 A^CGT
MamI          124  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI          423  CCAGG                CC^[AT]GG
NsiI          601  ATGCAT               ATGCA^T
NspI          621  ACATGT               [GA]CATG^[CT]
PstI          677  CTGCAG               CTGCA^G
RsaI          472  GTAC                 GT^AC
ScaI          471  AGTACT               AGT^ACT
ScrFI         423  CCAGG                CC^[ACGT]GG
TaqI           20  TCGA                 T^CGA
TfiI          333  GATTC                G^A[AT]TC
TseI          679  GCAGC                G^C[AT]GC
Tsp45I        572  GTGAC                ^GT[GC]AC
TspRI         615  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        20  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AhdI          728  GACAGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              766  GACATACTGTC         
AspEI         728  GACAGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              766  GACATACTGTC         
AvaII         465  GGACC                G^G[AT]CC
              873  GGACC               
BanII         154  GAGCTC               G[GA]GC[CT]^C
              507  GAGCTC              
BbsI           22  GAAGAC               GAAGAC(2/6)
              692  GTCTTC              
BcgI          593  CGATTACCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              327  GCAAGGGATTCG        
BpuAI          22  GAAGAC               GAAGAC(2/6)
              692  GTCTTC              
BsmFI         464  GGGAC                GGGAC(10/14)
              784  GGGAC               
BstAPI         70  GCAAACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              522  GCACAAGGTGC         
DpnII         358  GATC                 ^GATC
              480  GATC                
DraI          180  TTTAAA               TTT^AAA
              637  TTTAAA              
Eam1105I      728  GACAGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              766  GACATACTGTC         
HaeIII        210  GGCC                 GG^CC
              578  GGCC                
MboI          358  GATC                 ^GATC
              480  GATC                
NdeII         358  GATC                 ^GATC
              480  GATC                
PleI          377  GACTC                GAGTC(4/5)
              751  GACTC               
SacI          154  GAGCTC               GAGCT^C
              507  GAGCTC              
Sau3AI        358  GATC                 ^GATC
              480  GATC                
SfcI          677  CTGCAG               C^T[GA][CT]AG
              788  CTGTAG              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        AspHI       BmyI        BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        BstF5I      Cfr13I      
DdeI        FokI        HinfI       MaeIII      MboII       
MnlI        MseI        MwoI        NlaIII      NlaIV       
Sau96I      Tru9I       Tsp509I     
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