Restriction map for pF1KB5477
 (Linear) (Six-base) MAPSORT of: KIBB5477.seq  Check: 8470  from: 1  to: 1173



KIBB5477.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AclI AA'CG_TT

Cuts at:      0     648    1173 
   Size:        648     525
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337    1115    1173 
   Size:        337     778      58

  Fragments arranged by size: 

                 778     337      58
 
AflIII A'CryG_T

Cuts at:      0    1058    1173 
   Size:       1058     115
 
AhdI GACnn_n'nnGTC

Cuts at:      0     449    1173 
   Size:        449     724
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1150    1173 
   Size:       1150      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     315    1173 
   Size:        315     858
 
AvaI C'yCGr_G

Cuts at:      0     407     865    1173 
   Size:        407     458     308

  Fragments arranged by size: 

                 458     407     308
 
BamHI G'GATC_C

Cuts at:      0     267     402     467    1173 
   Size:        267     135      65     706

  Fragments arranged by size: 

                 706     267     135      65
 
BanI G'GyrC_C

Cuts at:      0     394    1000    1173 
   Size:        394     606     173

  Fragments arranged by size: 

                 606     394     173
 
BanII G_rGCy'C

Cuts at:      0     255    1091    1173 
   Size:        255     836      82

  Fragments arranged by size: 

                 836     255      82
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     515     731     859     958    1173 
   Size:        515     216     128      99     215

  Fragments arranged by size: 

                 515     216     215     128      99
 
BbvCI CC'TCA_GC

Cuts at:      0     701    1173 
   Size:        701     472
 
BlpI GC'TnA_GC

Cuts at:      0      98    1173 
   Size:         98    1075
 
Bme1580I G_kGCm'C

Cuts at:      0     399     579    1173 
   Size:        399     180     594

  Fragments arranged by size: 

                 594     399     180
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     625    1173 
   Size:        403     222     548

  Fragments arranged by size: 

                 548     403     222
 
Bpu10I CC'TnA_GC

Cuts at:      0     701     900    1173 
   Size:        701     199     273

  Fragments arranged by size: 

                 701     273     199
 
BsaWI w'CCGG_w

Cuts at:      0     136     202    1173 
   Size:        136      66     971

  Fragments arranged by size: 

                 971     136      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     862     892    1150    1173 
   Size:        862      30     258      23

  Fragments arranged by size: 

                 862     258      30      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     718     721     778     883    1158    1173 
   Size:        718       3      57     105     275      15

  Fragments arranged by size: 

                 718     275     105      57      15       3
 
BsiEI CG_ry'CG

Cuts at:      0    1047    1173 
   Size:       1047     126
 
Bsp1286I G_dGCh'C

Cuts at:      0     255     399     579    1091    1173 
   Size:        255     144     180     512      82

  Fragments arranged by size: 

                 512     255     180     144      82
 
BspEI T'CCGG_A

Cuts at:      0     202    1173 
   Size:        202     971
 
BsrBI CCG'CTC

Cuts at:      0     899    1173 
   Size:        899     274
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     977     986     995    1173 
   Size:        977       9       9     178

  Fragments arranged by size: 

                 977     178       9       9
 
BstYI r'GATC_y

Cuts at:      0     267     402     467    1173 
   Size:        267     135      65     706

  Fragments arranged by size: 

                 706     267     135      65
 
Bsu36I CC'TnA_GG

Cuts at:      0     263    1173 
   Size:        263     910
 
BtgI C'CryG_G

Cuts at:      0      59    1173 
   Size:         59    1114
 
BtsI GCAGTG_nn'

Cuts at:      0      73     579     953     973    1173 
   Size:         73     506     374      20     200

  Fragments arranged by size: 

                 506     374     200      73      20
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     185    1173 
   Size:        185     988
 
EaeI y'GGCC_r

Cuts at:      0     894    1173 
   Size:        894     279
 
EarI CTCTTCn'nnn_

Cuts at:      0    1033    1173 
   Size:       1033     140
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     252    1173 
   Size:        252     921
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     403     625    1115    1173 
   Size:        337      66     222     490      58

  Fragments arranged by size: 

                 490     337     222      66      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     319    1173 
   Size:        319     854
 
EcoO109I rG'GnC_Cy

Cuts at:      0     784    1173 
   Size:        784     389
 
FspI TGC'GCA

Cuts at:      0     370    1173 
   Size:        370     803
 
HaeII r_GCGC'y

Cuts at:      0     234    1173 
   Size:        234     939
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     435     467     653     685     757     789     862     894 
   Size:        435      32     186      32      72      32      73      32

Cuts at:    894    1173 
   Size:        279

  Fragments arranged by size: 

                 435     279     186      73      72      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0    1142    1173 
   Size:       1142      31
 
MfeI C'AATT_G

Cuts at:      0     153    1173 
   Size:        153    1020
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1173 
   Size:       1084      89
 
MslI CAynn'nnrTG

Cuts at:      0     166    1173 
   Size:        166    1007
 
MspA1I CmG'CkG

Cuts at:      0     210     962    1173 
   Size:        210     752     211

  Fragments arranged by size: 

                 752     211     210
 
NcoI C'CATG_G

Cuts at:      0      59    1173 
   Size:         59    1114
 
NspI r_CATG'y

Cuts at:      0    1062    1173 
   Size:       1062     111
 
PciI A'CATG_T

Cuts at:      0    1058    1173 
   Size:       1058     115
 
PfoI T'CCnGG_A

Cuts at:      0     189     488     602    1173 
   Size:        189     299     114     571

  Fragments arranged by size: 

                 571     299     189     114
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1150    1173 
   Size:       1150      23
 
PpuMI rG'GwC_Cy

Cuts at:      0     784    1173 
   Size:        784     389
 
PvuI CG_AT'CG

Cuts at:      0    1047    1173 
   Size:       1047     126
 
PvuII CAG'CTG

Cuts at:      0     210    1173 
   Size:        210     963
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1033    1173 
   Size:       1033     140
 
ScaI AGT'ACT

Cuts at:      0      46    1173 
   Size:         46    1127
 
StuI AGG'CCT

Cuts at:      0     668    1173 
   Size:        668     505
 
StyI C'CwwG_G

Cuts at:      0      33      59     554    1173 
   Size:         33      26     495     619

  Fragments arranged by size: 

                 619     495      33      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     753    1173 
   Size:        753     420
 
TatI w'GTAC_w

Cuts at:      0      44     212    1061    1173 
   Size:         44     168     849     112

  Fragments arranged by size: 

                 849     168     112      44
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AhdI     AloI    AlwNI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BlpI Bme1580I     BpmI   Bpu10I
    BsaWI    BsaXI    BseRI    BsiEI Bsp1286I    BspEI    BsrBI   BstAPI
    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I  HindIII     MfeI
     MmeI     MslI   MspA1I     NcoI     NspI     PciI     PfoI     PpiI
    PpuMI     PvuI    PvuII     SapI     ScaI     StuI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AleI
     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI
  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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