Restriction map for pF1KB5457
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     AscI        AseI        AsnI        AspEI       
AspI        AvaI        AviII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BfrI        BglII       BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
Ksp632I     KspI        MfeI        MflI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        TfiI        Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I        264  GGTACC               G^GTACC
Alw44I        556  GTGCAC               G^TGCAC
AlwI          209  GGATC                GGATC(4/5)
AlwNI         166  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          401  GGGCCC               GGGCC^C
ApaLI         556  GTGCAC               G^TGCAC
Asp700I       948  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       264  GGTACC               G^GTACC
AspHI         556  GTGCAC               G[AT]GC[AT]^C
AvrII        1116  CCTAGG               C^CTAGG
BciVI         276  GGATAC               GTATCC(6/5)
BlnI         1116  CCTAGG               C^CTAGG
BlpI         1333  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1333  GCTCAGC              GC^T[ACGT]AGC
BsaBI         696  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       556  GTGCAC               G[AT]GC[AT]^C
BsmI         1250  GCATTC               GAATGC(1/-1)
BspMI         746  ACCTGC               ACCTGC(4/8)
BsrBI         375  GAGCGG               CCGCTC(-3/-3)
BsrDI         477  CATTGC               GCAATG(2/0)
CelII        1333  GCTCAGC              GC^T[ACGT]AGC
DraIII        541  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI         890  GAATTC               G^AATTC
EcoT22I      1133  ATGCAT               ATGCA^T
KpnI          264  GGTACC               GGTAC^C
MaeII         620  ACGT                 A^CGT
MamI          696  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         1413  CCATGG               C^CATGG
NheI          898  GCTAGC               G^CTAGC
NsiI         1133  ATGCAT               ATGCA^T
NspI          581  ACATGC               [GA]CATG^[CT]
PpuMI         915  GGGTCCC              [GA]G^G[AT]CC[CT]
SspI          712  AATATT               AAT^ATT
StuI          653  AGGCCT               AGG^CCT
XmnI          948  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII          18  CGCG                 CG^CG
              771  CGCG                
BalI          128  TGGCCA               TGG^CCA
             1373  TGGCCA              
BanII         401  GGGCCC               G[GA]GC[CT]^C
             1352  GGGCTC              
BglI          130  GCCAATGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1412  GCCATGGTGGC         
BpmI          497  CTGGAG               CTGGAG(16/14)
              958  CTGGAG              
BsaI          341  GAGACC               GGTCTC(1/5)
              183  GGTCTC              
BstUI          18  CGCG                 CG^CG
              771  CGCG                
BstXI         149  CCAAAGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              930  CCAGTTTGATGG        
DdeI         1024  CTAAG                C^T[ACGT]AG
             1334  CTCAG               
DraII         400  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              915  GGGTCCC             
DsaI           72  CCACGG               C^C[GA][CT]GG
             1413  CCATGG              
EcoNI         219  CCTGGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1323  CCTCATCCAGG         
EcoO109I      400  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              915  GGGTCCC             
MluNI         128  TGGCCA               TGG^CCA
             1373  TGGCCA              
MscI          128  TGGCCA               TGG^CCA
             1373  TGGCCA              
MslI         1251  CATTCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1357  CACACCTATG          
MvnI           18  CGCG                 CG^CG
              771  CGCG                
PvuII         586  CAGCTG               CAG^CTG
             1190  CAGCTG              
TaqI          254  TCGA                 T^CGA
             1143  TCGA                
TthHB8I       254  TCGA                 T^CGA
             1143  TCGA                
XcmI          726  CCAAGACACCGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              803  CCATCTCCGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BanI        BbvI        BcnI        BfaI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DpnII       
EaeI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com