Restriction map for pF1KB5457
 (Linear) (Six-base) MAPSORT of: KIBB5457.seq  Check: 1602  from: 1  to: 1449



KIBB5457.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     755    1449 
   Size:        755     694
 
Acc65I G'GTAC_C

Cuts at:      0     264    1449 
   Size:        264    1185
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     898     931    1141    1449 
   Size:        214     684      33     210     308

  Fragments arranged by size: 

                 684     308     214     210      33
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     763    1273    1449 
   Size:        763     510     176

  Fragments arranged by size: 

                 763     510     176
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171    1449 
   Size:        171    1278
 
ApaI G_GGCC'C

Cuts at:      0     405    1449 
   Size:        405    1044
 
ApaLI G'TGCA_C

Cuts at:      0     556    1449 
   Size:        556     893
 
ApoI r'AATT_y

Cuts at:      0     306     359     890    1449 
   Size:        306      53     531     559

  Fragments arranged by size: 

                 559     531     306      53
 
AvrII C'CTAG_G

Cuts at:      0    1116    1449 
   Size:       1116     333
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     256     289    1449 
   Size:        256      33    1160

  Fragments arranged by size: 

                1160     256      33
 
BanI G'GyrC_C

Cuts at:      0     160     264     335    1420    1449 
   Size:        160     104      71    1085      29

  Fragments arranged by size: 

                1085     160     104      71      29
 
BanII G_rGCy'C

Cuts at:      0     405    1356    1449 
   Size:        405     951      93

  Fragments arranged by size: 

                 951     405      93
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     270    1449 
   Size:        270    1179
 
BfrBI ATG'CAT

Cuts at:      0    1135    1449 
   Size:       1135     314
 
BglI GCCn_nnn'nGGC

Cuts at:      0     136    1418    1449 
   Size:        136    1282      31

  Fragments arranged by size: 

                1282     136      31
 
BlpI GC'TnA_GC

Cuts at:      0    1334    1449 
   Size:       1334     115
 
Bme1580I G_kGCm'C

Cuts at:      0     165     405     560     787    1449 
   Size:        165     240     155     227     662

  Fragments arranged by size: 

                 662     240     227     165     155
 
BmgBI CAC'GTC

Cuts at:      0     621    1449 
   Size:        621     828
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     159     525    1244    1449 
   Size:        159     366     719     205

  Fragments arranged by size: 

                 719     366     205     159
 
BmtI G_CTAG'C

Cuts at:      0     902    1449 
   Size:        902     547
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     201     702    1449 
   Size:        201     501     747

  Fragments arranged by size: 

                 747     501     201
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     979    1449 
   Size:        518     461     470

  Fragments arranged by size: 

                 518     470     461
 
Bpu10I CC'TnA_GC

Cuts at:      0    1024    1449 
   Size:       1024     425
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     703     992    1449 
   Size:        331     372     289     457

  Fragments arranged by size: 

                 457     372     331     289
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     189     335    1449 
   Size:        189     146    1114

  Fragments arranged by size: 

                1114     189     146
 
BsaBI GATnn'nnATC

Cuts at:      0     700    1449 
   Size:        700     749
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     836     866    1449 
   Size:        836      30     583

  Fragments arranged by size: 

                 836     583      30
 
BseYI C'CCAG_C

Cuts at:      0     229     624    1449 
   Size:        229     395     825

  Fragments arranged by size: 

                 825     395     229
 
BsiHKAI G_wGCw'C

Cuts at:      0     560    1449 
   Size:        560     889
 
BsmI GAATG_Cn'

Cuts at:      0    1250    1449 
   Size:       1250     199
 
Bsp1286I G_dGCh'C

Cuts at:      0     165     405     560     787    1356    1449 
   Size:        165     240     155     227     569      93

  Fragments arranged by size: 

                 569     240     227     165     155      93
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     755    1449 
   Size:        755     694
 
BsrBI CCG'CTC

Cuts at:      0     377    1449 
   Size:        377    1072
 
BsrDI GCAATG_nn'

Cuts at:      0     476    1449 
   Size:        476     973
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     156     937    1449 
   Size:        156     781     512

  Fragments arranged by size: 

                 781     512     156
 
BtgI C'CryG_G

Cuts at:      0      72    1413    1449 
   Size:         72    1341      36

  Fragments arranged by size: 

                1341      72      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     401    1056    1449 
   Size:        401     655     393

  Fragments arranged by size: 

                 655     401     393
 
BtsI GCAGTG_nn'

Cuts at:      0    1271    1449 
   Size:       1271     178
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1026    1047    1061    1082    1449 
   Size:       1026      21      14      21     367

  Fragments arranged by size: 

                1026     367      21      21      14
 
DraIII CAC_nnn'GTG

Cuts at:      0     546    1449 
   Size:        546     903
 
EaeI y'GGCC_r

Cuts at:      0      32     128    1373    1449 
   Size:         32      96    1245      76

  Fragments arranged by size: 

                1245      96      76      32
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     222     798    1449 
   Size:        222     576     651

  Fragments arranged by size: 

                 651     576     222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     518     898     931     979    1141    1449 
   Size:        214     304     380      33      48     162     308

  Fragments arranged by size: 

                 380     308     304     214     162      48      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     223    1327    1449 
   Size:        223    1104     122

  Fragments arranged by size: 

                1104     223     122
 
EcoO109I rG'GnC_Cy

Cuts at:      0     401     916    1449 
   Size:        401     515     533

  Fragments arranged by size: 

                 533     515     401
 
EcoRI G'AATT_C

Cuts at:      0     890    1449 
   Size:        890     559
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     483    1449 
   Size:        483     966
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      20      52     836     868     934     966    1449 
   Size:         20      32     784      32      66      32     483

  Fragments arranged by size: 

                 784     483      66      32      32      32      20
 
KpnI G_GTAC'C

Cuts at:      0     268    1449 
   Size:        268    1181
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1449 
   Size:        254    1195
 
MscI TGG'CCA

Cuts at:      0     130    1375    1449 
   Size:        130    1245      74

  Fragments arranged by size: 

                1245     130      74
 
MslI CAynn'nnrTG

Cuts at:      0    1255    1361    1449 
   Size:       1255     106      88

  Fragments arranged by size: 

                1255     106      88
 
MspA1I CmG'CkG

Cuts at:      0     588    1192    1338    1449 
   Size:        588     604     146     111

  Fragments arranged by size: 

                 604     588     146     111
 
NcoI C'CATG_G

Cuts at:      0    1413    1449 
   Size:       1413      36
 
NheI G'CTAG_C

Cuts at:      0     898    1449 
   Size:        898     551
 
NsiI A_TGCA'T

Cuts at:      0    1137    1449 
   Size:       1137     312
 
NspI r_CATG'y

Cuts at:      0     585    1449 
   Size:        585     864
 
PfoI T'CCnGG_A

Cuts at:      0     956    1449 
   Size:        956     493
 
PpuMI rG'GwC_Cy

Cuts at:      0     916    1449 
   Size:        916     533
 
PspOMI G'GGCC_C

Cuts at:      0     401    1449 
   Size:        401    1048
 
PvuII CAG'CTG

Cuts at:      0     588    1192    1449 
   Size:        588     604     257

  Fragments arranged by size: 

                 604     588     257
 
SanDI GG'GwC_CC

Cuts at:      0     916    1449 
   Size:        916     533
 
SmlI C'TyrA_G

Cuts at:      0     346     718    1007    1449 
   Size:        346     372     289     442

  Fragments arranged by size: 

                 442     372     346     289
 
SspI AAT'ATT

Cuts at:      0     714    1449 
   Size:        714     735
 
StuI AGG'CCT

Cuts at:      0     655    1449 
   Size:        655     794
 
StyI C'CwwG_G

Cuts at:      0     353     471    1116    1413    1449 
   Size:        353     118     645     297      36

  Fragments arranged by size: 

                 645     353     297     118      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148     507    1449 
   Size:        148     359     942

  Fragments arranged by size: 

                 942     359     148
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     137     169    1449 
   Size:        137      32    1280

  Fragments arranged by size: 

                1280     137      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     733     810    1449 
   Size:        733      77     639

  Fragments arranged by size: 

                 733     639      77
 
XmnI GAAnn'nnTTC

Cuts at:      0     952    1449 
   Size:        952     497
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AlfI    AlwNI     ApaI    ApaLI     ApoI
    AvrII     BaeI     BanI    BanII    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaXI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrBI    BsrDI    BstXI     BtgI    BtgZI     BtsI    CspCI   DraIII
     EaeI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NheI     NsiI
     NspI     PfoI    PpuMI   PspOMI    PvuII    SanDI     SmlI     SspI
     StuI     StyI    TaqII     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     AscI     AseI    AsiSI     AvaI    BamHI     BbeI
     BbsI    BbvCI     BcgI     BclI    BglII    BsaAI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     ClaI     DraI     DrdI     EagI     EarI  EcoICRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI
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