Restriction map for pF1KB5400
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        KspI        MamI        MflI        MluI        
MroI        MvnI        NaeI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1225  GGTACC               G^GTACC
AfaI         1226  GTAC                 GT^AC
AocI          560  CCTCAGG              CC^T[ACGT]AGG
Asp718I      1225  GGTACC               G^GTACC
BalI          513  TGGCCA               TGG^CCA
BcgI         1137  GCAGGAGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          361  CTAG                 C^TAG
BsmAI         936  GAGAC                GTCTC(1/5)
BsmFI         702  GGGAC                GGGAC(10/14)
BsmI         1357  GCATTC               GAATGC(1/-1)
BssSI         875  CTCGTG               CACGAG(-5/-1)
BstBI         455  TTCGAA               TT^CGAA
Bsu36I        560  CCTCAGG              CC^T[ACGT]AGG
DpnII         547  GATC                 ^GATC
DraIII        398  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          513  TGGCCA               [CT]^GGCC[GA]
Eco81I        560  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       677  CCATGG               C^C[AT][AT]GG
HapII        1261  CCGG                 C^CGG
HpaII        1261  CCGG                 C^CGG
KpnI         1225  GGTACC               GGTAC^C
MaeI          361  CTAG                 C^TAG
MaeII        1401  ACGT                 A^CGT
MaeIII        664  GTGAC                ^GT[ACGT]AC
MboI          547  GATC                 ^GATC
MfeI         1337  CAATTG               C^AATTG
MluNI         513  TGGCCA               TGG^CCA
MscI          513  TGGCCA               TGG^CCA
MseI           20  TTAA                 T^TAA
MspI         1261  CCGG                 C^CGG
MunI         1337  CAATTG               C^AATTG
NcoI          677  CCATGG               C^CATGG
NdeII         547  GATC                 ^GATC
NspV          455  TTCGAA               TT^CGAA
PpuMI        1242  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI         1226  GTAC                 GT^AC
SacI         1021  GAGCTC               GAGCT^C
Sau3AI        547  GATC                 ^GATC
SexAI         920  ACCAGGT              A^CC[AT]GGT
SfcI          530  CTGTAG               C^T[GA][CT]AG
SfuI          455  TTCGAA               TT^CGAA
StyI          677  CCATGG               C^C[AT][AT]GG
Tru9I          20  TTAA                 T^TAA
Tsp45I        664  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcsI          180  GAATTT               [GA]^AATT[CT]
             1384  GAATTT              
AcyI          953  GGCGCC               G[GA]^CG[CT]C
             1046  GGCGCC              
AlwNI        1241  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1370  CAGCTCCTG           
ApoI          180  GAATTT               [GA]^AATT[CT]
             1384  GAATTT              
Asp700I       201  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              961  GAAGAACTTC          
AspHI         916  GAGCAC               G[AT]GC[AT]^C
             1021  GAGCTC              
BanII         673  GAGCCC               G[GA]GC[CT]^C
             1021  GAGCTC              
BbeI          953  GGCGCC               GGCGC^C
             1046  GGCGCC              
BbsI          201  GAAGAC               GAAGAC(2/6)
              307  GTCTTC              
BpuAI         201  GAAGAC               GAAGAC(2/6)
              307  GTCTTC              
BsaHI         953  GGCGCC               G[GA]^CG[CT]C
             1046  GGCGCC              
BsaJI         402  CCGTGG               C^C[ACGT][ACGT]GG
              677  CCATGG              
BseRI        1235  CTCCTC               GAGGAG(10/8)
             1349  CTCCTC              
BsiHKAI       916  GAGCAC               G[AT]GC[AT]^C
             1021  GAGCTC              
BspMI         123  ACCTGC               ACCTGC(4/8)
             1245  ACCTGC              
BsrI         1017  ACTGG                ACTGG(1/-1)
              684  CCAGT               
DraII         556  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1242  AGGACCT             
DsaI          402  CCGTGG               C^C[GA][CT]GG
              677  CCATGG              
EcoO109I      556  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1242  AGGACCT             
HaeII         953  GGCGCC               [GA]GCGC^[CT]
             1046  GGCGCC              
Hin1I         953  GGCGCC               G[GA]^CG[CT]C
             1046  GGCGCC              
HinfI        1106  GATTC                G^A[ACGT]TC
             1143  GATTC               
HphI           66  TCACC                GGTGA(8/7)
              209  TCACC               
KasI          953  GGCGCC               G^GCGCC
             1046  GGCGCC              
NarI          953  GGCGCC               GG^CGCC
             1046  GGCGCC              
PflMI         397  CCACTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              803  CCATGCGCTGG         
SapI          979  GAAGAGC              GCTCTTC(1/4)
             1036  GAAGAGC             
SfoI          953  GGCGCC               GGC^GCC
             1046  GGCGCC              
TaqI          456  TCGA                 T^CGA
              549  TCGA                
TfiI         1106  GATTC                G^A[AT]TC
             1143  GATTC               
TspRI         255  CACTG                CA[GC]TG[ACGT][ACGT]^
              624  CAGTG               
TthHB8I       456  TCGA                 T^CGA
              549  TCGA                
Van91I        397  CCACTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              803  CCATGCGCTGG         
XcmI          374  CCAAAGTGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              479  CCAACGAACTGCTGG     
XmnI          201  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              961  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AluI        AvaII       BanI        BbvI        
BmyI        BpmI        BsgI        BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
ItaI        Ksp632I     MboII       MnlI        MslI        
MspA1I      MvaI        MwoI        NlaIII      NlaIV       
PvuII       Sau96I      ScrFI       SfaNI       TseI        
Tsp509I     
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