Restriction map for pF1KB5400
 (Linear) (Six-base) MAPSORT of: KIBB5400.seq  Check: 1399  from: 1  to: 1413



KIBB5400.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

Acc65I G'GTAC_C

Cuts at:      0    1225    1413 
   Size:       1225     188
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196    1413 
   Size:        196    1217
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1246    1375    1413 
   Size:       1246     129      38

  Fragments arranged by size: 

                1246     129      38
 
ApoI r'AATT_y

Cuts at:      0     180    1384    1413 
   Size:        180    1204      29

  Fragments arranged by size: 

                1204     180      29
 
BanI G'GyrC_C

Cuts at:      0     953    1046    1195    1225    1413 
   Size:        953      93     149      30     188

  Fragments arranged by size: 

                 953     188     149      93      30
 
BanII G_rGCy'C

Cuts at:      0     677    1025    1413 
   Size:        677     348     388

  Fragments arranged by size: 

                 677     388     348
 
BbeI G_GCGC'C

Cuts at:      0     957    1050    1413 
   Size:        957      93     363

  Fragments arranged by size: 

                 957     363      93
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     208     300    1413 
   Size:        208      92    1113

  Fragments arranged by size: 

                1113     208      92
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1126    1160    1413 
   Size:       1126      34     253

  Fragments arranged by size: 

                1126     253      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     907    1039    1223    1397    1413 
   Size:        907     132     184     174      16

  Fragments arranged by size: 

                 907     184     174     132      16
 
Bpu10I CC'TnA_GC

Cuts at:      0    1024    1413 
   Size:       1024     389
 
BsaHI Gr'CG_yC

Cuts at:      0     954    1047    1413 
   Size:        954      93     366

  Fragments arranged by size: 

                 954     366      93
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1218    1248    1413 
   Size:       1218      30     165

  Fragments arranged by size: 

                1218     165      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1226    1340    1413 
   Size:       1226     114      73

  Fragments arranged by size: 

                1226     114      73
 
BseYI C'CCAG_C

Cuts at:      0    1230    1413 
   Size:       1230     183
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     890     970    1388    1413 
   Size:        890      80     418      25

  Fragments arranged by size: 

                 890     418      80      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     920    1025    1413 
   Size:        920     105     388

  Fragments arranged by size: 

                 920     388     105
 
BsmI GAATG_Cn'

Cuts at:      0    1357    1413 
   Size:       1357      56
 
Bsp1286I G_dGCh'C

Cuts at:      0     677     920    1025    1413 
   Size:        677     243     105     388

  Fragments arranged by size: 

                 677     388     243     105
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     132    1254    1413 
   Size:        132    1122     159

  Fragments arranged by size: 

                1122     159     132
 
BssSI C'ACGA_G

Cuts at:      0     875    1413 
   Size:        875     538
 
BstBI TT'CG_AA

Cuts at:      0     456    1413 
   Size:        456     957
 
Bsu36I CC'TnA_GG

Cuts at:      0     561    1413 
   Size:        561     852
 
BtgI C'CryG_G

Cuts at:      0     402     677    1413 
   Size:        402     275     736

  Fragments arranged by size: 

                 736     402     275
 
BtsI GCAGTG_nn'

Cuts at:      0     630    1413 
   Size:        630     783
 
DraIII CAC_nnn'GTG

Cuts at:      0     403    1413 
   Size:        403    1010
 
EaeI y'GGCC_r

Cuts at:      0     513    1413 
   Size:        513     900
 
EarI CTCTTCn'nnn_

Cuts at:      0     692     974    1031    1052    1413 
   Size:        692     282      57      21     361

  Fragments arranged by size: 

                 692     361     282      57      21
 
EcoICRI GAG'CTC

Cuts at:      0    1023    1413 
   Size:       1023     390
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     907    1039    1223    1397    1413 
   Size:        196     711     132     184     174      16

  Fragments arranged by size: 

                 711     196     184     174     132      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     557    1243    1413 
   Size:        557     686     170

  Fragments arranged by size: 

                 686     557     170
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     721     982    1134    1413 
   Size:        721     261     152     279

  Fragments arranged by size: 

                 721     279     261     152
 
HaeII r_GCGC'y

Cuts at:      0     957    1050    1413 
   Size:        957      93     363

  Fragments arranged by size: 

                 957     363      93
 
KasI G'GCGC_C

Cuts at:      0     953    1046    1413 
   Size:        953      93     367

  Fragments arranged by size: 

                 953     367      93
 
KpnI G_GTAC'C

Cuts at:      0    1229    1413 
   Size:       1229     184
 
MfeI C'AATT_G

Cuts at:      0    1337    1413 
   Size:       1337      76
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1413 
   Size:        503     910
 
MscI TGG'CCA

Cuts at:      0     515    1413 
   Size:        515     898
 
MslI CAynn'nnrTG

Cuts at:      0     826     931    1255    1413 
   Size:        826     105     324     158

  Fragments arranged by size: 

                 826     324     158     105
 
MspA1I CmG'CkG

Cuts at:      0     414     598    1222    1413 
   Size:        414     184     624     191

  Fragments arranged by size: 

                 624     414     191     184
 
NarI GG'CG_CC

Cuts at:      0     954    1047    1413 
   Size:        954      93     366

  Fragments arranged by size: 

                 954     366      93
 
NcoI C'CATG_G

Cuts at:      0     677    1413 
   Size:        677     736
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     403     809    1413 
   Size:        403     406     604

  Fragments arranged by size: 

                 604     406     403
 
PfoI T'CCnGG_A

Cuts at:      0    1239    1374    1413 
   Size:       1239     135      39

  Fragments arranged by size: 

                1239     135      39
 
PpuMI rG'GwC_Cy

Cuts at:      0    1243    1413 
   Size:       1243     170
 
PsiI TTA'TAA

Cuts at:      0     724    1413 
   Size:        724     689
 
PvuII CAG'CTG

Cuts at:      0     414     598    1222    1413 
   Size:        414     184     624     191

  Fragments arranged by size: 

                 624     414     191     184
 
SacI G_AGCT'C

Cuts at:      0    1025    1413 
   Size:       1025     388
 
SapI GCTCTTCn'nnn_

Cuts at:      0     974    1031    1413 
   Size:        974      57     382

  Fragments arranged by size: 

                 974     382      57
 
SexAI A'CCwGG_T

Cuts at:      0     920    1413 
   Size:        920     493
 
SfcI C'TryA_G

Cuts at:      0     530    1413 
   Size:        530     883
 
SfoI GGC'GCC

Cuts at:      0     955    1048    1413 
   Size:        955      93     365

  Fragments arranged by size: 

                 955     365      93
 
StyI C'CwwG_G

Cuts at:      0     677    1413 
   Size:        677     736
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     777    1413 
   Size:        226     551     636

  Fragments arranged by size: 

                 636     551     226
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381     486    1413 
   Size:        381     105     927

  Fragments arranged by size: 

                 927     381     105
 
XmnI GAAnn'nnTTC

Cuts at:      0     205     965    1413 
   Size:        205     760     448

  Fragments arranged by size: 

                 760     448     205
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI    BanII     BbeI     BbsI
     BcgI     BpmI   Bpu10I    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BssSI    BstBI   Bsu36I     BtgI
     BtsI   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI    PflMI     PfoI    PpuMI     PsiI    PvuII     SacI
     SapI    SexAI     SfcI     SfoI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com