Restriction map for pF1KB7894
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        Alw44I      ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspI        AvrII       
BbeI        BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsiWI       BsmAI       
BsmBI       Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MseI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        1536  GACGTC               GACGT^C
AccIII        164  TCCGGA               T^CCGGA
AflIII       1485  ACGTGT               A^C[GA][CT]GT
AocI          808  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1508  AGCGCT               AGC^GCT
AviII        1865  TGCGCA               TGC^GCA
BamHI         858  GGATCC               G^GATCC
BbrPI        1484  CACGTG               CAC^GTG
BfaI          238  CTAG                 C^TAG
BglI          702  GCCCCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1448  AGATCT               A^GATCT
BseAI         164  TCCGGA               T^CCGGA
BsiEI        1962  CGACCG               CG[GA][CT]^CG
BspEI         164  TCCGGA               T^CCGGA
BspHI         116  TCATGA               T^CATGA
BstAPI       1389  GCAGGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        808  CCTGAGG              CC^T[ACGT]AGG
Eco47III     1508  AGCGCT               AGC^GCT
Eco81I        808  CCTGAGG              CC^T[ACGT]AGG
EcoNI         867  CCTGTATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1865  TGCGCA               TGC^GCA
MaeI          238  CTAG                 C^TAG
MroI          164  TCCGGA               T^CCGGA
MslI         1869  CACAGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI        1119  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1484  CACGTG               CAC^GTG
PmlI         1484  CACGTG               CAC^GTG
PshAI         538  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          116  TCATGA               T^CATGA
SacI         1720  GAGCTC               GAGCT^C
SapI         1267  GAAGAGC              GCTCTTC(1/4)
ScaI          650  AGTACT               AGT^ACT
SfiI          701  GGCCCCGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         1360  CTTGAG               C^T[CT][GA]AG
StuI          331  AGGCCT               AGG^CCT
TaqI         1289  TCGA                 T^CGA
TthHB8I      1289  TCGA                 T^CGA
Van91I       1119  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1533  CCAGACGTCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AhdI           46  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1102  GACCCTGCGTC         
AlwNI        1120  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1636  CAGGTGCTG           
Asp700I       570  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1291  GAACGCATTC          
AspEI          46  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1102  GACCCTGCGTC         
BalI          182  TGGCCA               TGG^CCA
              443  TGGCCA              
BanI         1469  GGCACC               G^G[CT][GA]CC
             1694  GGTGCC              
BsaWI          99  ACCGGA               [AT]^CCGG[AT]
              164  TCCGGA              
BseRI        1024  GAGGAG               GAGGAG(10/8)
             1408  GAGGAG              
BsmI           69  GAATGC               GAATGC(1/-1)
             1295  GCATTC              
BsrBI         271  GAGCGG               CCGCTC(-3/-3)
              286  GAGCGG              
BsrFI         608  GCCGGC               [GA]^CCGG[CT]
              707  GCCGGC              
Cfr10I        608  GCCGGC               [GA]^CCGG[CT]
              707  GCCGGC              
DsaI          150  CCATGG               C^C[GA][CT]GG
              903  CCATGG              
Eam1105I       46  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1102  GACCCTGCGTC         
MluNI         182  TGGCCA               TGG^CCA
              443  TGGCCA              
MscI          182  TGGCCA               TGG^CCA
              443  TGGCCA              
MspA1I        625  CAGCTG               C[AC]G^C[GT]G
             1676  CAGCTG              
NaeI          608  GCCGGC               GCC^GGC
              707  GCCGGC              
NcoI          150  CCATGG               C^CATGG
              903  CCATGG              
NgoMIV        608  GCCGGC               G^CCGGC
              707  GCCGGC              
PstI          352  CTGCAG               CTGCA^G
             1444  CTGCAG              
PvuII         625  CAGCTG               CAG^CTG
             1676  CAGCTG              
SmaI          305  CCCGGG               CCC^GGG
              635  CCCGGG              
XmaCI         305  CCCGGG               C^CCGGG
              635  CCCGGG              
XmaI          305  CCCGGG               C^CCGGG
              635  CCCGGG              
XmnI          570  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1291  GAACGCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaAI       BsaHI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        TseI        
Tsp45I      TspRI       XhoII       
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