Restriction map for
pF1KB5311
(Linear) (Six-base) MAPSORT of: KIBB5311.seq Check: 7675 from: 1 to: 1398
KIBB5311.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 571 1398
Size: 277 294 827
Fragments arranged by size:
827 294 277
AflII C'TTAA_G
Cuts at: 0 1327 1398
Size: 1327 71
AflIII A'CryG_T
Cuts at: 0 137 1070 1398
Size: 137 933 328
Fragments arranged by size:
933 328 137
AleI CACnn'nnGTG
Cuts at: 0 370 1398
Size: 370 1028
AlwNI CAG_nnn'CTG
Cuts at: 0 103 1152 1242 1398
Size: 103 1049 90 156
Fragments arranged by size:
1049 156 103 90
ApaLI G'TGCA_C
Cuts at: 0 1096 1398
Size: 1096 302
ApoI r'AATT_y
Cuts at: 0 293 1084 1398
Size: 293 791 314
Fragments arranged by size:
791 314 293
AvaI C'yCGr_G
Cuts at: 0 717 1398
Size: 717 681
BanI G'GyrC_C
Cuts at: 0 1078 1377 1398
Size: 1078 299 21
Fragments arranged by size:
1078 299 21
BanII G_rGCy'C
Cuts at: 0 242 1145 1398
Size: 242 903 253
Fragments arranged by size:
903 253 242
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 853 1398
Size: 457 396 545
Fragments arranged by size:
545 457 396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1160 1194 1398
Size: 1160 34 204
Fragments arranged by size:
1160 204 34
BclI T'GATC_A
Cuts at: 0 789 1398
Size: 789 609
BlpI GC'TnA_GC
Cuts at: 0 907 1398
Size: 907 491
Bme1580I G_kGCm'C
Cuts at: 0 1100 1219 1398
Size: 1100 119 179
Fragments arranged by size:
1100 179 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 572 1398
Size: 572 826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1398
Size: 88 1310
Bpu10I CC'TnA_GC
Cuts at: 0 662 1398
Size: 662 736
BsaI GGTCTCn'nnnn_
Cuts at: 0 158 1398
Size: 158 1240
BsaAI yAC'GTr
Cuts at: 0 336 1398
Size: 336 1062
BsaBI GATnn'nnATC
Cuts at: 0 1126 1398
Size: 1126 272
BsaWI w'CCGG_w
Cuts at: 0 918 1398
Size: 918 480
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 1024 1398
Size: 618 406 374
Fragments arranged by size:
618 406 374
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1380 1398
Size: 455 925 18
Fragments arranged by size:
925 455 18
BsiEI CG_ry'CG
Cuts at: 0 958 1398
Size: 958 440
BsiHKAI G_wGCw'C
Cuts at: 0 1100 1398
Size: 1100 298
BsmI GAATG_Cn'
Cuts at: 0 835 903 1398
Size: 835 68 495
Fragments arranged by size:
835 495 68
Bsp1286I G_dGCh'C
Cuts at: 0 242 1100 1145 1219 1398
Size: 242 858 45 74 179
Fragments arranged by size:
858 242 179 74 45
BspEI T'CCGG_A
Cuts at: 0 918 1398
Size: 918 480
BspHI T'CATG_A
Cuts at: 0 485 1398
Size: 485 913
BsrDI GCAATG_nn'
Cuts at: 0 1196 1398
Size: 1196 202
BsrGI T'GTAC_A
Cuts at: 0 140 1398
Size: 140 1258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 78 1242 1398
Size: 78 1164 156
Fragments arranged by size:
1164 156 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 876 1398
Size: 876 522
BstYI r'GATC_y
Cuts at: 0 347 1103 1398
Size: 347 756 295
Fragments arranged by size:
756 347 295
BtgI C'CryG_G
Cuts at: 0 242 1398
Size: 242 1156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 421 1398
Size: 421 977
EaeI y'GGCC_r
Cuts at: 0 94 287 767 955 1398
Size: 94 193 480 188 443
Fragments arranged by size:
480 443 193 188 94
EagI C'GGCC_G
Cuts at: 0 955 1398
Size: 955 443
EarI CTCTTCn'nnn_
Cuts at: 0 230 961 1398
Size: 230 731 437
Fragments arranged by size:
731 437 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 277 571 1398
Size: 88 189 294 827
Fragments arranged by size:
827 294 189 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 161 1398
Size: 161 1237
EcoRI G'AATT_C
Cuts at: 0 293 1398
Size: 293 1105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 346 1398
Size: 346 1052
HaeII r_GCGC'y
Cuts at: 0 52 1398
Size: 52 1346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 711 743 774 806 1398
Size: 91 32 588 32 31 32 592
Fragments arranged by size:
592 588 91 32 32 32 31
MfeI C'AATT_G
Cuts at: 0 600 1398
Size: 600 798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 776 1398
Size: 80 696 622
Fragments arranged by size:
696 622 80
MscI TGG'CCA
Cuts at: 0 289 769 1398
Size: 289 480 629
Fragments arranged by size:
629 480 289
MslI CAynn'nnrTG
Cuts at: 0 370 1398
Size: 370 1028
MspA1I CmG'CkG
Cuts at: 0 396 1242 1398
Size: 396 846 156
Fragments arranged by size:
846 396 156
NcoI C'CATG_G
Cuts at: 0 242 1398
Size: 242 1156
NspI r_CATG'y
Cuts at: 0 42 141 1074 1398
Size: 42 99 933 324
Fragments arranged by size:
933 324 99 42
PciI A'CATG_T
Cuts at: 0 137 1070 1398
Size: 137 933 328
Fragments arranged by size:
933 328 137
PmlI CAC'GTG
Cuts at: 0 336 1398
Size: 336 1062
PstI C_TGCA'G
Cuts at: 0 83 401 671 727 1238 1247 1398
Size: 83 318 270 56 511 9 151
Fragments arranged by size:
511 318 270 151 83 56 9
PvuII CAG'CTG
Cuts at: 0 396 1242 1398
Size: 396 846 156
Fragments arranged by size:
846 396 156
SapI GCTCTTCn'nnn_
Cuts at: 0 230 1398
Size: 230 1168
SfcI C'TryA_G
Cuts at: 0 79 397 475 667 723 1234 1243 1398
Size: 79 318 78 192 56 511 9 155
Fragments arranged by size:
511 318 192 155 79 78 56 9
SmlI C'TyrA_G
Cuts at: 0 7 1327 1398
Size: 7 1320 71
Fragments arranged by size:
1320 71 7
SphI G_CATG'C
Cuts at: 0 42 1398
Size: 42 1356
StyI C'CwwG_G
Cuts at: 0 242 632 961 1062 1398
Size: 242 390 329 101 336
Fragments arranged by size:
390 336 329 242 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 1365 1398
Size: 145 1220 33
Fragments arranged by size:
1220 145 33
TatI w'GTAC_w
Cuts at: 0 140 749 1398
Size: 140 609 649
Fragments arranged by size:
649 609 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1398
Size: 179 32 1187
Fragments arranged by size:
1187 179 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1041 1398
Size: 1041 357
XmnI GAAnn'nnTTC
Cuts at: 0 821 1398
Size: 821 577
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BcgI BclI BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrGI
BstAPI BstXI BstYI BtgI BtgZI EaeI EagI EarI
Eco57MI EcoNI EcoRI FalI HaeII Hin4I MfeI MmeI
MscI MslI MspA1I NcoI NspI PciI PmlI PstI
PvuII SapI SfcI SmlI SphI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BfrBI BglI BglII BmgBI
BmtI BplI BpuEI BsaHI BsaXI BseYI BsiWI BsmBI
BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoO109I EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
ZraI