(Linear) (Six-base) MAPSORT of: KIBB5166.seq  Check: 3543  from: 1  to: 474



KIBB5166.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     148     474 
   Size:         20     128     326

  Fragments arranged by size: 

                 326     128      20
 
BanI G'GyrC_C

Cuts at:      0     206     474 
   Size:        206     268
 
BbeI G_GCGC'C

Cuts at:      0     210     474 
   Size:        210     264
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     232     474 
   Size:        232     242
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     268     474 
   Size:        268     206
 
Bpu10I CC'TnA_GC

Cuts at:      0     163     474 
   Size:        163     311
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     280     474 
   Size:        280     194
 
BsaHI Gr'CG_yC

Cuts at:      0     207     474 
   Size:        207     267
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     397     474 
   Size:        397      77
 
BseYI C'CCAG_C

Cuts at:      0     189     474 
   Size:        189     285
 
BsrFI r'CCGG_y

Cuts at:      0     185     474 
   Size:        185     289
 
BssSI C'ACGA_G

Cuts at:      0     151     474 
   Size:        151     323
 
BstYI r'GATC_y

Cuts at:      0      65     474 
   Size:         65     409
 
EaeI y'GGCC_r

Cuts at:      0       8     474 
   Size:          8     466
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     474 
   Size:         16     458
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     148     474 
   Size:         20     128     326

  Fragments arranged by size: 

                 326     128      20
 
FspI TGC'GCA

Cuts at:      0     282     474 
   Size:        282     192
 
HaeII r_GCGC'y

Cuts at:      0     210     474 
   Size:        210     264
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     234     266     392     424     474 
   Size:        234      32     126      32      50

  Fragments arranged by size: 

                 234     126      50      32      32
 
KasI G'GCGC_C

Cuts at:      0     206     474 
   Size:        206     268
 
MslI CAynn'nnrTG

Cuts at:      0     386     474 
   Size:        386      88
 
NaeI GCC'GGC

Cuts at:      0     187     474 
   Size:        187     287
 
NarI GG'CG_CC

Cuts at:      0     207     474 
   Size:        207     267
 
NgoMIV G'CCGG_C

Cuts at:      0     185     474 
   Size:        185     289
 
NspI r_CATG'y

Cuts at:      0      89     474 
   Size:         89     385
 
PstI C_TGCA'G

Cuts at:      0      65     474 
   Size:         65     409
 
SapI GCTCTTCn'nnn_

Cuts at:      0      16     474 
   Size:         16     458
 
ScaI AGT'ACT

Cuts at:      0     145     474 
   Size:        145     329
 
SfcI C'TryA_G

Cuts at:      0      61     474 
   Size:         61     413
 
SfoI GGC'GCC

Cuts at:      0     208     474 
   Size:        208     266
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     181     474 
   Size:        181     293
 
TatI w'GTAC_w

Cuts at:      0     143     474 
   Size:        143     331
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     106     474 
   Size:        106     368
 
XmnI GAAnn'nnTTC

Cuts at:      0     382     474 
   Size:        382      92
 

 Enzymes that do cut:

     AcuI     BanI     BbeI     BmrI     BplI   Bpu10I     BsaI    BsaHI
    BseRI    BseYI    BsrFI    BssSI    BstYI     EaeI     EarI  Eco57MI
     FspI    HaeII    Hin4I     KasI     MslI     NaeI     NarI   NgoMIV
     NspI     PstI     SapI     ScaI     SfcI     SfoI    TaqII     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BpmI    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI