Restriction map for pF1KB5046
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsgI        BsiEI       BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoT22I     FbaI        FseI        FspI        HpaI        
KasI        KpnI        MaeI        MaeII       MamI        
MfeI        MluI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII        601  TCCGGA               T^CCGGA
AspHI         886  GAGCTC               G[AT]GC[AT]^C
AvaI          415  CCCGGG               C^[CT]CG[GA]G
BalI          191  TGGCCA               TGG^CCA
BanI         1117  GGTGCC               G^G[CT][GA]CC
BciVI          91  GTATCC               GTATCC(6/5)
BglI          733  GCCCTTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         601  TCCGGA               [AT]^CCGG[AT]
BseAI         601  TCCGGA               T^CCGGA
BsiHKAI       886  GAGCTC               G[AT]GC[AT]^C
BsmAI        1129  GTCTC                GTCTC(1/5)
BsmBI        1128  CGTCTC               CGTCTC(1/5)
BsmFI         715  GTCCC                GGGAC(10/14)
BsmI          915  GAATGC               GAATGC(1/-1)
BsoBI         415  CCCGGG               C^[CT]CG[GA]G
BspEI         601  TCCGGA               T^CCGGA
BsrDI         134  GCAATG               GCAATG(2/0)
BssSI         874  CTCGTG               CACGAG(-5/-1)
BstEII        960  GGTGACC              G^GT[ACGT]ACC
BstPI         960  GGTGACC              G^GT[ACGT]ACC
EaeI          191  TGGCCA               [CT]^GGCC[GA]
EarI          883  GAAGAG               CTCTTC(1/4)
EcoNI         668  CCTTAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       960  GGTGACC              G^GT[ACGT]ACC
EcoRV         388  GATATC               GAT^ATC
HincII        520  GTCAAC               GT[CT]^[GA]AC
HindII        520  GTCAAC               GT[CT]^[GA]AC
HindIII       216  AAGCTT               A^AGCTT
Ksp632I       883  GAAGAG               CTCTTC(1/4)
KspI          972  CCGCGG               CCGC^GG
MluNI         191  TGGCCA               TGG^CCA
MroI          601  TCCGGA               T^CCGGA
MscI          191  TGGCCA               TGG^CCA
NspI           57  ACATGC               [GA]CATG^[CT]
PleI         1163  GAGTC                GAGTC(4/5)
PvuII        1083  CAGCTG               CAG^CTG
SacI          886  GAGCTC               GAGCT^C
SacII         972  CCGCGG               CCGC^GG
SapI          883  GAAGAGC              GCTCTTC(1/4)
SmaI          415  CCCGGG               CCC^GGG
TfiI          585  GATTC                G^A[AT]TC
Tsp509I       552  AATT                 ^AATT
XmaCI         415  CCCGGG               C^CCGGG
XmaI          415  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         829  CGCG                 CG^CG
              973  CGCG                
AcyI          745  GGCGTC               G[GA]^CG[CT]C
             1126  GGCGTC              
AfaI           67  GTAC                 GT^AC
              870  GTAC                
AlwI          489  GATCC                GGATC(4/5)
              693  GATCC               
BsaHI         745  GGCGTC               G[GA]^CG[CT]C
             1126  GGCGTC              
BstUI         829  CGCG                 CG^CG
              973  CGCG                
BstYI         488  AGATCC               [GA]^GATC[CT]
              692  AGATCC              
DdeI          252  CTCAG                C^T[ACGT]AG
             1140  CTCAG               
DsaI           62  CCGTGG               C^C[GA][CT]GG
              972  CCGCGG              
EcoT14I       533  CCAAGG               C^C[AT][AT]GG
              570  CCAAGG              
HaeII         314  AGCGCC               [GA]GCGC^[CT]
              436  AGCGCC              
Hin1I         745  GGCGTC               G[GA]^CG[CT]C
             1126  GGCGTC              
HinfI         585  GATTC                G^A[ACGT]TC
             1163  GAGTC               
MboII         657  GAAGA                GAAGA(8/7)
              883  GAAGA               
MflI          488  AGATCC               [GA]^GATC[CT]
              692  AGATCC              
MseI          554  TTAA                 T^TAA
              670  TTAA                
MvnI          829  CGCG                 CG^CG
              973  CGCG                
RsaI           67  GTAC                 GT^AC
              870  GTAC                
StyI          533  CCAAGG               C^C[AT][AT]GG
              570  CCAAGG              
Tru9I         554  TTAA                 T^TAA
              670  TTAA                
TspRI          83  CACTG                CA[GC]TG[ACGT][ACGT]^
              206  CACTG               
XcmI          533  CCAAGGAGGATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              728  CCAGGGCCCTTGTGG     
XhoII         488  AGATCC               [GA]^GATC[CT]
              692  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr13I      DpnII       DraII       
DrdI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        TseI        Tsp45I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com