Restriction map for pF1KB5018
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AviII       AvrII       BalI        
BamHI       BbrPI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     CelII       
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        NciI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       TspRI       XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         393  GACGTC               GACGT^C
Acc65I        250  GGTACC               G^GTACC
AcsI          602  GAATTC               [GA]^AATT[CT]
AfaI          251  GTAC                 GT^AC
AlwI           48  GATCC                GGATC(4/5)
AocI          323  CCTCAGG              CC^T[ACGT]AGG
ApoI          602  GAATTC               [GA]^AATT[CT]
Asp718I       250  GGTACC               G^GTACC
AspI          169  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          616  CCCGAG               C^[CT]CG[GA]G
AvaII         705  GGACC                G^G[AT]CC
BbeI          901  GGCGCC               GGCGC^C
BbsI          451  GAAGAC               GAAGAC(2/6)
BfaI          469  CTAG                 C^TAG
BglII         158  AGATCT               A^GATCT
BpuAI         451  GAAGAC               GAAGAC(2/6)
BseRI         913  GAGGAG               GAGGAG(10/8)
BsoBI         616  CCCGAG               C^[CT]CG[GA]G
BspDI         196  ATCGAT               AT^CGAT
BsrBI         532  GAGCGG               CCGCTC(-3/-3)
BsrI          714  CCAGT                ACTGG(1/-1)
BstEII        225  GGTGACC              G^GT[ACGT]ACC
BstPI         225  GGTGACC              G^GT[ACGT]ACC
Bsu36I        323  CCTCAGG              CC^T[ACGT]AGG
ClaI          196  ATCGAT               AT^CGAT
EarI          426  CTCTTC               CTCTTC(1/4)
Eco81I        323  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       225  GGTGACC              G^GT[ACGT]ACC
EcoRI         602  GAATTC               G^AATTC
EcoT14I       515  CCATGG               C^C[AT][AT]GG
HaeII         901  GGCGCC               [GA]GCGC^[CT]
KasI          901  GGCGCC               G^GCGCC
KpnI          250  GGTACC               GGTAC^C
Ksp632I       426  CTCTTC               CTCTTC(1/4)
MaeI          469  CTAG                 C^TAG
MaeII         394  ACGT                 A^CGT
NarI          901  GGCGCC               GG^CGCC
NcoI          515  CCATGG               C^CATGG
PflFI         169  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         774  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           28  CTGCAG               CTGCA^G
RsaI          251  GTAC                 GT^AC
SfoI          901  GGCGCC               GGC^GCC
SmlI          162  CTTGAG               C^T[CT][GA]AG
StyI          515  CCATGG               C^C[AT][AT]GG
TaqI          197  TCGA                 T^CGA
Tsp509I       603  AATT                 ^AATT
Tth111I       169  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       197  TCGA                 T^CGA
Van91I        774  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         456  CGCG                 CG^CG
             1023  CGCG                
BbvCI         254  CCTCAGC              CCTCAGC(-5/-2)
              796  CCTCAGC             
BpmI          772  CTCCAG               CTGGAG(16/14)
              691  CTGGAG              
BsmAI          67  GAGAC                GTCTC(1/5)
              490  GAGAC               
BsmBI          67  GAGACG               CGTCTC(1/5)
              490  GAGACG              
BspMI         345  ACCTGC               ACCTGC(4/8)
              732  GCAGGT              
BstUI         456  CGCG                 CG^CG
             1023  CGCG                
BstYI          47  AGATCC               [GA]^GATC[CT]
              158  AGATCT              
CfoI          754  GCGC                 GCG^C
              902  GCGC                
DraII         917  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              980  AGGCCCT             
EcoO109I      917  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              980  AGGCCCT             
HgaI          454  GACGC                GACGC(5/10)
              945  GACGC               
HhaI          754  GCGC                 GCG^C
              902  GCGC                
HinP1I        754  GCGC                 G^CGC
              902  GCGC                
MflI           47  AGATCC               [GA]^GATC[CT]
              158  AGATCT              
MvnI          456  CGCG                 CG^CG
             1023  CGCG                
NspI          329  GCATGT               [GA]CATG^[CT]
              725  ACATGC              
PvuII         281  CAGCTG               CAG^CTG
              778  CAGCTG              
SfcI           28  CTGCAG               C^T[GA][CT]AG
              965  CTATAG              
StuI          269  AGGCCT               AGG^CCT
             1013  AGGCCT              
TfiI          379  GAATC                G^A[AT]TC
              738  GATTC               
XhoII          47  AGATCC               [GA]^GATC[CT]
              158  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcyI        AluI        AlwNI       BanI        
BanII       BbvI        BmyI        BsaHI       BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrFI       
BssKI       BstF5I      BstNI       Cac8I       Cfr10I      
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       Hin1I       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NaeI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        Tsp45I      
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