Restriction map for pF1KB4977
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaHI       BsaI        BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MfeI        MluI        MroI        MspA1I      
MunI        MvnI        NarI        NciI        NdeI        
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        TseI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI           18  GTCGAC               GT^[AC][GT]AC
AcsI          589  AAATTC               [GA]^AATT[CT]
AlwNI          61  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          589  AAATTC               [GA]^AATT[CT]
AspI          118  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         455  GGACC                G^G[AT]CC
BalI          561  TGGCCA               TGG^CCA
BfaI          503  CTAG                 C^TAG
BlpI           66  GCTGAGC              GC^T[ACGT]AGC
BmyI          700  GGGCAC               G[AGT]GC[ACT]^C
BpmI          101  CTGGAG               CTGGAG(16/14)
Bpu1102I       66  GCTGAGC              GC^T[ACGT]AGC
BsaAI         412  TACGTG               [CT]AC^GT[GA]
BsaJI         564  CCATGG               C^C[ACGT][ACGT]GG
BsaWI         395  TCCGGT               [AT]^CCGG[AT]
BsiYI         283  CCATTGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          283  CCATTGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         104  GAGACG               CGTCTC(1/5)
BsmI          813  GAATGC               GAATGC(1/-1)
Bsp1286I      700  GGGCAC               G[AGT]GC[ACT]^C
BsrFI          27  GCCGGC               [GA]^CCGG[CT]
BstAPI       1119  GCAAAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII          66  GCTGAGC              GC^T[ACGT]AGC
CfoI           25  GCGC                 GCG^C
Cfr10I         27  GCCGGC               [GA]^CCGG[CT]
Cfr13I        455  GGACC                G^G[ACGT]CC
DsaI          564  CCATGG               C^C[GA][CT]GG
EcoT14I       564  CCATGG               C^C[AT][AT]GG
FauI           31  GCGGG                CCCGC(4/6)
Fnu4HI         49  GCGGC                GC^[ACGT]GC
HgaI          118  GACGC                GACGC(5/10)
HhaI           25  GCGC                 GCG^C
HinP1I         25  GCGC                 G^CGC
HincII         18  GTCGAC               GT[CT]^[GA]AC
HindII         18  GTCGAC               GT[CT]^[GA]AC
HphI          742  GGTGA                GGTGA(8/7)
ItaI           49  GCGGC                GC^[ACGT]GC
MaeI          503  CTAG                 C^TAG
MaeII         413  ACGT                 A^CGT
MluNI         561  TGGCCA               TGG^CCA
MscI          561  TGGCCA               TGG^CCA
NaeI           27  GCCGGC               GCC^GGC
NcoI          564  CCATGG               C^CATGG
NgoMIV         27  GCCGGC               G^CCGGC
NspI           41  ACATGC               [GA]CATG^[CT]
PflFI         118  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         283  CCATTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI           18  GTCGAC               G^TCGAC
Sau96I        455  GGACC                G^G[ACGT]CC
SgrAI          26  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI          262  CTCAAG               C^T[CT][GA]AG
StyI          564  CCATGG               C^C[AT][AT]GG
TaqI           19  TCGA                 T^CGA
Tth111I       118  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        19  TCGA                 T^CGA
Van91I        283  CCATTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          502  TCTAGA               T^CTAGA
XcmI          279  CCAACCATTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AciI           31  GCGG                 CCGC(-3/-1)
               49  GCGG                
AfaI          140  GTAC                 GT^AC
             1092  GTAC                
BssKI         187  CCTGG                ^CC[ACGT]GG
              997  CCTGG               
BstNI         187  CCTGG                CC^[AT]GG
              997  CCTGG               
EaeI           50  CGGCCA               [CT]^GGCC[GA]
              561  TGGCCA              
EcoRII        187  CCTGG                ^CC[AT]GG
              997  CCTGG               
HapII          28  CCGG                 C^CGG
              396  CCGG                
HinfI         826  GAATC                G^A[ACGT]TC
             1039  GAATC               
HpaII          28  CCGG                 C^CGG
              396  CCGG                
MspI           28  CCGG                 C^CGG
              396  CCGG                
MvaI          187  CCTGG                CC^[AT]GG
              997  CCTGG               
MwoI          467  GCAATACGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1119  GCAAAAGATGC         
RsaI          140  GTAC                 GT^AC
             1092  GTAC                
ScrFI         187  CCTGG                CC^[ACGT]GG
              997  CCTGG               
SfcI          344  CTGTAG               C^T[GA][CT]AG
              491  CTGTAG              
SspI          236  AATATT               AAT^ATT
              681  AATATT              
TfiI          826  GAATC                G^A[AT]TC
             1039  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        AlwI        BsaBI       BsmAI       BsrI        
BstF5I      BstYI       Cac8I       DdeI        DpnII       
FokI        HaeIII      MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
NdeII       NlaIII      Sau3AI      SfaNI       Tru9I       
Tsp45I      Tsp509I     TspRI       XhoII       
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