Restriction map for pF1KB4976
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       AvrII       BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsgI        BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HindIII     HpaI        KasI        
KspI        MfeI        MluI        MseI        MspA1I      
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I       1225  GGTACC               G^GTACC
AccIII        503  TCCGGA               T^CCGGA
AcyI         1107  GACGCC               G[GA]^CG[CT]C
AlwNI        1180  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          394  GGGCCC               GGGCC^C
Asp718I      1225  GGTACC               G^GTACC
AspHI         328  GAGCAC               G[AT]GC[AT]^C
BalI          242  TGGCCA               TGG^CCA
BamHI         129  GGATCC               G^GATCC
BanII         394  GGGCCC               G[GA]GC[CT]^C
BcgI          787  GCACCCAGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI        1107  GACGCC               G[GA]^CG[CT]C
BsaI          553  GAGACC               GGTCTC(1/5)
BsaWI         503  TCCGGA               [AT]^CCGG[AT]
BseAI         503  TCCGGA               T^CCGGA
BsiHKAI       328  GAGCAC               G[AT]GC[AT]^C
BsmBI        1105  GAGACG               CGTCTC(1/5)
BspEI         503  TCCGGA               T^CCGGA
BspMI         172  ACCTGC               ACCTGC(4/8)
BsrBI         148  GAGCGG               CCGCTC(-3/-3)
BssSI          97  CTCGTG               CACGAG(-5/-1)
BstXI        1019  CCAGCGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI         483  GAATTC               G^AATTC
Hin1I        1107  GACGCC               G[GA]^CG[CT]C
HincII        646  GTCAAC               GT[CT]^[GA]AC
HindII        646  GTCAAC               GT[CT]^[GA]AC
HinfI         155  GAGTC                G^A[ACGT]TC
KpnI         1225  GGTACC               GGTAC^C
MluNI         242  TGGCCA               TGG^CCA
MroI          503  TCCGGA               T^CCGGA
MscI          242  TGGCCA               TGG^CCA
MslI         1047  CACCCTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI          155  GAGTC                GAGTC(4/5)
SmlI          877  CTCAAG               C^T[CT][GA]AG
StuI          529  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI           58  GTCTAC               GT^[AC][GT]AC
              811  GTCTAC              
AccII         293  CGCG                 CG^CG
              450  CGCG                
AcsI          456  GAATTT               [GA]^AATT[CT]
              483  GAATTC              
ApoI          456  GAATTT               [GA]^AATT[CT]
              483  GAATTC              
BbsI          400  GAAGAC               GAAGAC(2/6)
              886  GAAGAC              
BbvI          387  GCAGC                GCAGC(8/12)
              297  GCTGC               
BcnI          192  CCGGG                CC^[GC]GG
              391  CCGGG               
BpuAI         400  GAAGAC               GAAGAC(2/6)
              886  GAAGAC              
BsaBI         690  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1117  GATACCAATC          
BseRI         463  GAGGAG               GAGGAG(10/8)
             1216  GAGGAG              
BsmFI          13  GGGAC                GGGAC(10/14)
               74  GGGAC               
BsrFI         264  ACCGGC               [GA]^CCGG[CT]
             1228  ACCGGC              
BstUI         293  CGCG                 CG^CG
              450  CGCG                
Cfr10I        264  ACCGGC               [GA]^CCGG[CT]
             1228  ACCGGC              
DrdI          199  GACCTGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              427  GACTATGAAGTC        
EarI          766  GAAGAG               CTCTTC(1/4)
             1213  GAAGAG              
Ksp632I       766  GAAGAG               CTCTTC(1/4)
             1213  GAAGAG              
MamI          690  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1117  GATACCAATC          
MvnI          293  CGCG                 CG^CG
              450  CGCG                
NciI          192  CCGGG                CC^[GC]GG
              391  CCGGG               
NcoI          627  CCATGG               C^CATGG
              761  CCATGG              
PstI          298  CTGCAG               CTGCA^G
              385  CTGCAG              
SfaNI        1015  GCATC                GCATC(5/9)
             1083  GCATC               
SfcI          298  CTGCAG               C^T[GA][CT]AG
              385  CTGCAG              
TaqI           92  TCGA                 T^CGA
              796  TCGA                
TseI          297  GCTGC                G^C[AT]GC
              387  GCAGC               
Tsp509I       457  AATT                 ^AATT
              484  AATT                
TthHB8I        92  TCGA                 T^CGA
              796  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
AvaII       BanI        BfaI        BglII       BmyI        
BsaJI       BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       StyI        Tsp45I      TspRI       XhoII       
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