Restriction map for pF1KB4822
 (Linear) (Six-base) MAPSORT of: KIBB4822.seq  Check: 4902  from: 1  to: 1353



KIBB4822.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1127    1353 
   Size:       1127     226
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     870    1353 
   Size:         81     789     483

  Fragments arranged by size: 

                 789     483      81
 
ApaI G_GGCC'C

Cuts at:      0    1016    1353 
   Size:       1016     337
 
AvaI C'yCGr_G

Cuts at:      0     112     811    1353 
   Size:        112     699     542

  Fragments arranged by size: 

                 699     542     112
 
BanII G_rGCy'C

Cuts at:      0     971    1016    1353 
   Size:        971      45     337

  Fragments arranged by size: 

                 971     337      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     286    1353 
   Size:        286    1067
 
BglI GCCn_nnn'nGGC

Cuts at:      0     815    1353 
   Size:        815     538
 
BglII A'GATC_T

Cuts at:      0    1324    1353 
   Size:       1324      29
 
BlpI GC'TnA_GC

Cuts at:      0     922    1353 
   Size:        922     431
 
Bme1580I G_kGCm'C

Cuts at:      0     225     672    1016    1353 
   Size:        225     447     344     337

  Fragments arranged by size: 

                 447     344     337     225
 
BmgBI CAC'GTC

Cuts at:      0     803    1353 
   Size:        803     550
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1014    1353 
   Size:       1014     339
 
BmtI G_CTAG'C

Cuts at:      0    1228    1353 
   Size:       1228     125
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     442     552     952    1069    1353 
   Size:         28     414     110     400     117     284

  Fragments arranged by size: 

                 414     400     284     117     110      28
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1049    1353 
   Size:       1049     304
 
BsaAI yAC'GTr

Cuts at:      0     842    1212    1353 
   Size:        842     370     141

  Fragments arranged by size: 

                 842     370     141
 
BsaHI Gr'CG_yC

Cuts at:      0     200     848    1353 
   Size:        200     648     505

  Fragments arranged by size: 

                 648     505     200
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1111    1141    1353 
   Size:       1111      30     212

  Fragments arranged by size: 

                1111     212      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     208    1180    1353 
   Size:        148      60     972     173

  Fragments arranged by size: 

                 972     173     148      60
 
BseYI C'CCAG_C

Cuts at:      0     483     488     621    1353 
   Size:        483       5     133     732

  Fragments arranged by size: 

                 732     483     133       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739    1353 
   Size:        739     614
 
BsiEI CG_ry'CG

Cuts at:      0      21     525    1035    1125    1353 
   Size:         21     504     510      90     228

  Fragments arranged by size: 

                 510     504     228      90      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     225     672     971    1016    1353 
   Size:        225     447     299      45     337

  Fragments arranged by size: 

                 447     337     299     225      45
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     483    1072    1353 
   Size:        483     589     281

  Fragments arranged by size: 

                 589     483     281
 
BsrBI CCG'CTC

Cuts at:      0     336    1127    1353 
   Size:        336     791     226

  Fragments arranged by size: 

                 791     336     226
 
BsrDI GCAATG_nn'

Cuts at:      0     318    1353 
   Size:        318    1035
 
BsrFI r'CCGG_y

Cuts at:      0     749    1034    1353 
   Size:        749     285     319

  Fragments arranged by size: 

                 749     319     285
 
BsrGI T'GTAC_A

Cuts at:      0     371    1353 
   Size:        371     982
 
BssSI C'ACGA_G

Cuts at:      0     784    1353 
   Size:        784     569
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     858    1047    1353 
   Size:        858     189     306

  Fragments arranged by size: 

                 858     306     189
 
BstYI r'GATC_y

Cuts at:      0     824     998    1324    1353 
   Size:        824     174     326      29

  Fragments arranged by size: 

                 824     326     174      29
 
Bsu36I CC'TnA_GG

Cuts at:      0     386    1273    1353 
   Size:        386     887      80

  Fragments arranged by size: 

                 887     386      80
 
BtgI C'CryG_G

Cuts at:      0     600     831    1119    1353 
   Size:        600     231     288     234

  Fragments arranged by size: 

                 600     288     234     231
 
DraI TTT'AAA

Cuts at:      0     231    1353 
   Size:        231    1122
 
EaeI y'GGCC_r

Cuts at:      0      18     603     894    1122    1353 
   Size:         18     585     291     228     231

  Fragments arranged by size: 

                 585     291     231     228      18
 
EagI C'GGCC_G

Cuts at:      0      18    1122    1353 
   Size:         18    1104     231

  Fragments arranged by size: 

                1104     231      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     133     214    1353 
   Size:        133      81    1139

  Fragments arranged by size: 

                1139     133      81
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      41      61    1321    1353 
   Size:         41      20    1260      32

  Fragments arranged by size: 

                1260      41      32      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     442     552     952    1069    1353 
   Size:         28     414     110     400     117     284

  Fragments arranged by size: 

                 414     400     284     117     110      28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     787     862    1353 
   Size:        787      75     491

  Fragments arranged by size: 

                 787     491      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     426     867    1013    1353 
   Size:        426     441     146     340

  Fragments arranged by size: 

                 441     426     340     146
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     162    1353 
   Size:        162    1191
 
HaeII r_GCGC'y

Cuts at:      0    1024    1353 
   Size:       1024     329
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     236     268    1353 
   Size:        236      32    1085

  Fragments arranged by size: 

                1085     236      32
 
MscI TGG'CCA

Cuts at:      0     896    1353 
   Size:        896     457
 
MslI CAynn'nnrTG

Cuts at:      0     842    1353 
   Size:        842     511
 
MspA1I CmG'CkG

Cuts at:      0     101     345     602    1353 
   Size:        101     244     257     751

  Fragments arranged by size: 

                 751     257     244     101
 
NaeI GCC'GGC

Cuts at:      0     751    1353 
   Size:        751     602
 
NcoI C'CATG_G

Cuts at:      0     831    1353 
   Size:        831     522
 
NgoMIV G'CCGG_C

Cuts at:      0     749    1353 
   Size:        749     604
 
NheI G'CTAG_C

Cuts at:      0    1224    1353 
   Size:       1224     129
 
PfoI T'CCnGG_A

Cuts at:      0     545     690    1353 
   Size:        545     145     663

  Fragments arranged by size: 

                 663     545     145
 
PpuMI rG'GwC_Cy

Cuts at:      0     426     867    1353 
   Size:        426     441     486

  Fragments arranged by size: 

                 486     441     426
 
PspOMI G'GGCC_C

Cuts at:      0    1012    1353 
   Size:       1012     341
 
PvuII CAG'CTG

Cuts at:      0     345    1353 
   Size:        345    1008
 
SacII CC_GC'GG

Cuts at:      0     603    1353 
   Size:        603     750
 
SapI GCTCTTCn'nnn_

Cuts at:      0     133     214    1353 
   Size:        133      81    1139

  Fragments arranged by size: 

                1139     133      81
 
SexAI A'CCwGG_T

Cuts at:      0     863    1353 
   Size:        863     490
 
SmaI CCC'GGG

Cuts at:      0     114    1353 
   Size:        114    1239
 
SnaBI TAC'GTA

Cuts at:      0     842    1353 
   Size:        842     511
 
StyI C'CwwG_G

Cuts at:      0     731     831    1353 
   Size:        731     100     522

  Fragments arranged by size: 

                 731     522     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     539    1353 
   Size:        539     814
 
TatI w'GTAC_w

Cuts at:      0     371     767    1353 
   Size:        371     396     586

  Fragments arranged by size: 

                 586     396     371
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1111    1143    1353 
   Size:       1111      32     210

  Fragments arranged by size: 

                1111     210      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     541     801     865    1353 
   Size:        541     260      64     488

  Fragments arranged by size: 

                 541     488     260      64
 
XmaI C'CCGG_G

Cuts at:      0     112    1353 
   Size:        112    1241
 

 Enzymes that do cut:

     AlfI    AlwNI     ApaI     AvaI    BanII     BbsI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI    BstXI    BstYI   Bsu36I
     BtgI     DraI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NheI     PfoI    PpuMI   PspOMI    PvuII    SacII
     SapI    SexAI     SmaI    SnaBI     StyI    TaqII     TatI     TstI
  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BplI   Bpu10I    BpuEI    BsaBI
    BsaWI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NarI     NdeI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XcmI     XhoI
     XmnI     ZraI
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