Restriction map for pF1KB4809
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     AvrII       BamHI       
BbsI        BciVI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaI        BseAI       BsiWI       
BspDI       BspEI       BspHI       BspLU11I    BsrFI       
BssHII      BstBI       Bsu36I      Cfr10I      ClaI        
DraI        Eco47III    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KspI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflMI       PinAI       
PleI        PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        SacI        SacII       SalI        
SbfI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tru9I       Van91I      XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         1204  GTATAC               GT^[AC][GT]AC
AcsI         1275  GAATTT               [GA]^AATT[CT]
AflIII       1208  ACACGT               A^C[GA][CT]GT
ApaI         1775  GGGCCC               GGGCC^C
ApoI         1275  GAATTT               [GA]^AATT[CT]
AspI          763  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          562  CTCGAG               C^[CT]CG[GA]G
AviII        1850  TGCGCA               TGC^GCA
BbeI          608  GGCGCC               GGCGC^C
BbrPI        1618  CACGTG               CAC^GTG
BbvCI        1866  CCTCAGC              CCTCAGC(-5/-2)
BfaI         1138  CTAG                 C^TAG
BlpI         2013  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2013  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1618  CACGTG               [CT]AC^GT[GA]
BsaBI        1548  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1960  TCCGGT               [AT]^CCGG[AT]
BsgI         1615  CTGCAC               GTGCAG(16/14)
BsmBI        1489  GAGACG               CGTCTC(1/5)
BsmI          407  GCATTC               GAATGC(1/-1)
BsoBI         562  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      263  TGTACA               T^GTACA
BsrGI         263  TGTACA               T^GTACA
BssSI        1786  CACGAG               CACGAG(-5/-1)
Bst1107I     1204  GTATAC               GTA^TAC
BstXI        1298  CCATGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1204  GTATAC               GTA^TAC
CelII        2013  GCTGAGC              GC^T[ACGT]AGC
CpoI          479  CGGTCCG              CG^G[AT]CCG
EagI         1476  CGGCCG               C^GGCCG
EarI         1927  CTCTTC               CTCTTC(1/4)
EclXI        1476  CGGCCG               C^GGCCG
Eco52I       1476  CGGCCG               C^GGCCG
FspI         1850  TGCGCA               TGC^GCA
HinfI           8  GATTC                G^A[ACGT]TC
KasI          608  GGCGCC               G^GCGCC
Ksp632I      1927  CTCTTC               CTCTTC(1/4)
MaeI         1138  CTAG                 C^TAG
MamI         1548  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          608  GGCGCC               GG^CGCC
PaeR7I        562  CTCGAG               C^TCGAG
PflFI         763  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1618  CACGTG               CAC^GTG
PmlI         1618  CACGTG               CAC^GTG
RsrII         479  CGGTCCG              CG^G[AT]CCG
SapI         1926  GCTCTTC              GCTCTTC(1/4)
ScaI          200  AGTACT               AGT^ACT
SexAI         440  ACCTGGT              A^CC[AT]GGT
SfoI          608  GGCGCC               GGC^GCC
SphI         2171  GCATGC               GCATG^C
SspBI         263  TGTACA               T^GTACA
TfiI            8  GATTC                G^A[AT]TC
Tsp509I      1276  AATT                 ^AATT
Tth111I       763  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          614  CCAAATGCCCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          562  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I        752  GGTACC               G^GTACC
             1963  GGTACC              
AhdI          427  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1261  GACCCTTCGTC         
Asp718I       752  GGTACC               G^GTACC
             1963  GGTACC              
AspEI         427  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1261  GACCCTTCGTC         
BcgI          564  CGAGGGCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              458  GCATCATCATCG        
BglI          560  GCCTCGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1894  GCCACAGAGGC         
BsiEI         138  CGACCG               CG[GA][CT]^CG
             1476  CGGCCG              
BsmAI        1118  GAGAC                GTCTC(1/5)
             1489  GAGAC               
BspMI         284  ACCTGC               ACCTGC(4/8)
             1127  ACCTGC              
BsrBI        1806  CCGCTC               CCGCTC(-3/-3)
             1770  GAGCGG              
BsrDI         543  CATTGC               GCAATG(2/0)
             1056  CATTGC              
BsrI          203  ACTGG                ACTGG(1/-1)
              510  CCAGT               
BstEII        327  GGTGACC              G^GT[ACGT]ACC
              786  GGTTACC             
BstPI         327  GGTGACC              G^GT[ACGT]ACC
              786  GGTTACC             
DraIII        439  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2125  CACAAGGTG           
Eam1105I      427  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1261  GACCCTTCGTC         
EcoO65I       327  GGTGACC              G^GT[ACGT]ACC
              786  GGTTACC             
HgaI           52  GACGC                GACGC(5/10)
              391  GACGC               
KpnI          752  GGTACC               GGTAC^C
             1963  GGTACC              
NcoI         1298  CCATGG               C^CATGG
             1382  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaII       BalI        
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DrdI        DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
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