Restriction map for pF1KB4800
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AlwI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
AspI        BamHI       BbsI        BbvCI       BciVI       
BclI        BfrI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KspI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        838  GGTACC               G^GTACC
AlwNI         231  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          428  GGGCCC               GGGCC^C
Asp718I       838  GGTACC               G^GTACC
AviII         821  TGCGCA               TGC^GCA
AvrII         588  CCTAGG               C^CTAGG
BbrPI         817  CACGTG               CAC^GTG
BcgI          869  GCAAGACCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          589  CTAG                 C^TAG
BglI          798  GCCGCACGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         944  AGATCT               A^GATCT
BlnI          588  CCTAGG               C^CTAGG
BsaAI         817  CACGTG               [CT]AC^GT[GA]
BsmI         1126  GCATTC               GAATGC(1/-1)
BsrBI         372  GAGCGG               CCGCTC(-3/-3)
BsrI         1087  ACTGG                ACTGG(1/-1)
BstYI         944  AGATCT               [GA]^GATC[CT]
DdeI         1002  CTCAG                C^T[ACGT]AG
DpnII         945  GATC                 ^GATC
EagI          491  CGGCCG               C^GGCCG
EclXI         491  CGGCCG               C^GGCCG
Eco52I        491  CGGCCG               C^GGCCG
EcoNI         583  CCTGGCCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       588  CCTAGG               C^C[AT][AT]GG
FspI          821  TGCGCA               TGC^GCA
KpnI          838  GGTACC               GGTAC^C
MaeI          589  CTAG                 C^TAG
MaeII         818  ACGT                 A^CGT
MaeIII        279  GTCAC                ^GT[ACGT]AC
MboI          945  GATC                 ^GATC
MflI          944  AGATCT               [GA]^GATC[CT]
MslI         1084  CACACTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          520  GCCGGC               GCC^GGC
NdeII         945  GATC                 ^GATC
NgoMIV        520  GCCGGC               G^CCGGC
PaeR7I        808  CTCGAG               C^TCGAG
PleI          718  GAGTC                GAGTC(4/5)
PmaCI         817  CACGTG               CAC^GTG
PmlI          817  CACGTG               CAC^GTG
PpuMI         826  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          552  CTGCAG               CTGCA^G
SapI          954  GAAGAGC              GCTCTTC(1/4)
Sau3AI        945  GATC                 ^GATC
ScaI         1028  AGTACT               AGT^ACT
SexAI         401  ACCTGGT              A^CC[AT]GGT
SfcI          552  CTGCAG               C^T[GA][CT]AG
SmaI          152  CCCGGG               CCC^GGG
SmlI          808  CTCGAG               C^T[CT][GA]AG
StyI          588  CCTAGG               C^C[AT][AT]GG
TaqI          809  TCGA                 T^CGA
TfiI          172  GAATC                G^A[AT]TC
Tsp45I        279  GTCAC                ^GT[GC]AC
TthHB8I       809  TCGA                 T^CGA
XhoI          808  CTCGAG               C^TCGAG
XhoII         944  AGATCT               [GA]^GATC[CT]
XmaCI         152  CCCGGG               C^CCGGG
XmaI          152  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AhdI          157  GACCGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              604  GACTTCGGGTC         
Asp700I       954  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1206  GAAGGGTTTC          
AspEI         157  GACCGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              604  GACTTCGGGTC         
BalI          483  TGGCCA               TGG^CCA
              675  TGGCCA              
BbeI           99  GGCGCC               GGCGC^C
              540  GGCGCC              
BsiEI         418  CGACCG               CG[GA][CT]^CG
              491  CGGCCG              
BsmAI        1246  GAGAC                GTCTC(1/5)
              291  GTCTC               
BsmBI         290  CGTCTC               CGTCTC(1/5)
             1246  GAGACG              
DraIII        400  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              883  CACGCGGTG           
DsaI          641  CCACGG               C^C[GA][CT]GG
              678  CCACGG              
Eam1105I      157  GACCGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              604  GACTTCGGGTC         
EarI          457  CTCTTC               CTCTTC(1/4)
              954  GAAGAG              
HinfI         172  GAATC                G^A[ACGT]TC
              718  GAGTC               
KasI           99  GGCGCC               G^GCGCC
              540  GGCGCC              
Ksp632I       457  CTCTTC               CTCTTC(1/4)
              954  GAAGAG              
MluNI         483  TGGCCA               TGG^CCA
              675  TGGCCA              
MscI          483  TGGCCA               TGG^CCA
              675  TGGCCA              
NarI           99  GGCGCC               GG^CGCC
              540  GGCGCC              
PvuII         178  CAGCTG               CAG^CTG
              219  CAGCTG              
SacI          246  GAGCTC               GAGCT^C
             1137  GAGCTC              
SfaNI         208  GCATC                GCATC(5/9)
              770  GCATC               
SfoI           99  GGCGCC               GGC^GCC
              540  GGCGCC              
TspRI         977  CACTG                CA[GC]TG[ACGT][ACGT]^
             1086  CACTG               
XmnI          954  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1206  GAAGGGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      ApaLI       AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BssKI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       TseI        
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