Restriction map for pF1KB4637
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaBI       
BsaHI       BseRI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT14I     EcoT22I     FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MscI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I        187  GGTACC               G^GTACC
AccIII        788  TCCGGA               T^CCGGA
AcsI           88  AAATTC               [GA]^AATT[CT]
AhdI          226  GACATCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          535  GGATC                GGATC(4/5)
AlwNI         251  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           88  AAATTC               [GA]^AATT[CT]
Asp700I       885  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       187  GGTACC               G^GTACC
AspEI         226  GACATCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          187  GGTACC               G^G[CT][GA]CC
BanII         876  GGGCTC               G[GA]GC[CT]^C
BbsI           76  GAAGAC               GAAGAC(2/6)
BclI          558  TGATCA               T^GATCA
BpuAI          76  GAAGAC               GAAGAC(2/6)
BsaAI         442  TACGTG               [CT]AC^GT[GA]
BsaI          448  GAGACC               GGTCTC(1/5)
BsaWI         788  TCCGGA               [AT]^CCGG[AT]
BseAI         788  TCCGGA               T^CCGGA
BsgI           93  CTGCAC               GTGCAG(16/14)
BsiEI         726  CGGCCG               CG[GA][CT]^CG
BspEI         788  TCCGGA               T^CCGGA
BsrBI         864  CCGCTC               CCGCTC(-3/-3)
BsrDI         647  GCAATG               GCAATG(2/0)
BsrFI         724  GCCGGC               [GA]^CCGG[CT]
BstYI         535  GGATCT               [GA]^GATC[CT]
Cfr10I        724  GCCGGC               [GA]^CCGG[CT]
DraII         689  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI           71  CCGTGG               C^C[GA][CT]GG
EaeI          726  CGGCCG               [CT]^GGCC[GA]
EagI          726  CGGCCG               C^GGCCG
Eam1105I      226  GACATCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          360  GAAGAG               CTCTTC(1/4)
EclXI         726  CGGCCG               C^GGCCG
Eco52I        726  CGGCCG               C^GGCCG
EcoO109I      689  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         475  GATATC               GAT^ATC
FauI          214  GCGGG                CCCGC(4/6)
FbaI          558  TGATCA               T^GATCA
KpnI          187  GGTACC               GGTAC^C
Ksp632I       360  GAAGAG               CTCTTC(1/4)
MaeII         443  ACGT                 A^CGT
MflI          535  GGATCT               [GA]^GATC[CT]
MroI          788  TCCGGA               T^CCGGA
MseI          151  TTAA                 T^TAA
MslI          660  CATCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          724  GCCGGC               GCC^GGC
NgoMIV        724  GCCGGC               G^CCGGC
PaeR7I        528  CTCGAG               C^TCGAG
PpuMI         689  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         594  GACTATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          277  CTGCAG               CTGCA^G
SfaNI         382  GATGC                GCATC(5/9)
SfcI          277  CTGCAG               C^T[GA][CT]AG
SmlI          528  CTCGAG               C^T[CT][GA]AG
Tru9I         151  TTAA                 T^TAA
Tsp509I        89  AATT                 ^AATT
XhoI          528  CTCGAG               C^TCGAG
XhoII         535  GGATCT               [GA]^GATC[CT]
XmnI          885  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          188  GTAC                 GT^AC
              402  GTAC                
AvaI          504  CTCGGG               C^[CT]CG[GA]G
              528  CTCGAG              
AvaII          68  GGACC                G^G[AT]CC
              690  GGACC               
BmyI          241  GTGCCC               G[AGT]GC[ACT]^C
              876  GGGCTC              
BsmAI         448  GAGAC                GTCTC(1/5)
              253  GTCTC               
BsoBI         504  CTCGGG               C^[CT]CG[GA]G
              528  CTCGAG              
Bsp1286I      241  GTGCCC               G[AGT]GC[ACT]^C
              876  GGGCTC              
BssSI         456  CACGAG               CACGAG(-5/-1)
              694  CTCGTG              
BstF5I        123  CATCC                GGATG(2/0)
              849  CATCC               
Cfr13I         68  GGACC                G^G[ACGT]CC
              690  GGACC               
FokI          123  CATCC                GGATG(9/13)
              849  CATCC               
HaeIII        108  GGCC                 GG^CC
              727  GGCC                
HgaI          211  GACGC                GACGC(5/10)
              232  GCGTC               
HphI          553  TCACC                GGTGA(8/7)
              768  TCACC               
MspA1I        261  CCGCTG               C[AC]G^C[GT]G
              431  CCGCTG              
RsaI          188  GTAC                 GT^AC
              402  GTAC                
Sau96I         68  GGACC                G^G[ACGT]CC
              690  GGACC               
TaqI          529  TCGA                 T^CGA
              740  TCGA                
TfiI          682  GATTC                G^A[AT]TC
              765  GATTC               
Tsp45I        552  GTCAC                ^GT[GC]AC
              697  GTGAC               
TthHB8I       529  TCGA                 T^CGA
              740  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BsmFI       BspMI       
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        DdeI        DpnII       EcoRII      Fnu4HI      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
ScrFI       TseI        TspRI       
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