Restriction map for pF1KB4637
 (Linear) (Six-base) MAPSORT of: KIBB4637.seq  Check: 3922  from: 1  to: 900



KIBB4637.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     284     606     900 
   Size:        284     322     294

  Fragments arranged by size: 

                 322     294     284
 
Acc65I G'GTAC_C

Cuts at:      0     187     900 
   Size:        187     713
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     439     602     900 
   Size:        367      72     163     298

  Fragments arranged by size: 

                 367     298     163      72
 
AhdI GACnn_n'nnGTC

Cuts at:      0     231     900 
   Size:        231     669
 
AlwNI CAG_nnn'CTG

Cuts at:      0     256     900 
   Size:        256     644
 
ApoI r'AATT_y

Cuts at:      0      88     900 
   Size:         88     812
 
AvaI C'yCGr_G

Cuts at:      0     504     528     900 
   Size:        504      24     372

  Fragments arranged by size: 

                 504     372      24
 
BanI G'GyrC_C

Cuts at:      0     187     900 
   Size:        187     713
 
BanII G_rGCy'C

Cuts at:      0     880     900 
   Size:        880      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     900 
   Size:         83     817
 
BclI T'GATC_A

Cuts at:      0     558     900 
   Size:        558     342
 
Bme1580I G_kGCm'C

Cuts at:      0     245     900 
   Size:        245     655
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      44     302     900 
   Size:         44     258     598

  Fragments arranged by size: 

                 598     258      44
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      60     900 
   Size:         60     840
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     442     900 
   Size:        442     458
 
BsaAI yAC'GTr

Cuts at:      0     444     900 
   Size:        444     456
 
BsaWI w'CCGG_w

Cuts at:      0     788     900 
   Size:        788     112
 
BseYI C'CCAG_C

Cuts at:      0     163     206     490     509     900 
   Size:        163      43     284      19     391

  Fragments arranged by size: 

                 391     284     163      43      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     900 
   Size:         78     822
 
BsiEI CG_ry'CG

Cuts at:      0     729     900 
   Size:        729     171
 
Bsp1286I G_dGCh'C

Cuts at:      0     245     880     900 
   Size:        245     635      20

  Fragments arranged by size: 

                 635     245      20
 
BspEI T'CCGG_A

Cuts at:      0     788     900 
   Size:        788     112
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     284     606     709     900 
   Size:        284     322     103     191

  Fragments arranged by size: 

                 322     284     191     103
 
BsrBI CCG'CTC

Cuts at:      0     866     900 
   Size:        866      34
 
BsrDI GCAATG_nn'

Cuts at:      0     654     900 
   Size:        654     246
 
BsrFI r'CCGG_y

Cuts at:      0     724     900 
   Size:        724     176
 
BssSI C'ACGA_G

Cuts at:      0     456     694     900 
   Size:        456     238     206

  Fragments arranged by size: 

                 456     238     206
 
BstYI r'GATC_y

Cuts at:      0     535     900 
   Size:        535     365
 
BtgI C'CryG_G

Cuts at:      0      71     900 
   Size:         71     829
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     213     900 
   Size:        213     687
 
BtsI GCAGTG_nn'

Cuts at:      0     179     900 
   Size:        179     721
 
EaeI y'GGCC_r

Cuts at:      0     726     900 
   Size:        726     174
 
EagI C'GGCC_G

Cuts at:      0     726     900 
   Size:        726     174
 
EarI CTCTTCn'nnn_

Cuts at:      0     355     900 
   Size:        355     545
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     841     900 
   Size:        841      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     439     602     900 
   Size:        367      72     163     298

  Fragments arranged by size: 

                 367     298     163      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0     690     900 
   Size:        690     210
 
EcoRV GAT'ATC

Cuts at:      0     477     900 
   Size:        477     423
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     217     249     487     511     519     543     900 
   Size:         27     190      32     238      24       8      24     357

  Fragments arranged by size: 

                 357     238     190      32      27      24      24       8
            
 
KpnI G_GTAC'C

Cuts at:      0     191     900 
   Size:        191     709
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     884     900 
   Size:        884      16
 
MslI CAynn'nnrTG

Cuts at:      0     664     900 
   Size:        664     236
 
MspA1I CmG'CkG

Cuts at:      0     263     433     900 
   Size:        263     170     467

  Fragments arranged by size: 

                 467     263     170
 
NaeI GCC'GGC

Cuts at:      0     726     900 
   Size:        726     174
 
NgoMIV G'CCGG_C

Cuts at:      0     724     900 
   Size:        724     176
 
PfoI T'CCnGG_A

Cuts at:      0     880     893     900 
   Size:        880      13       7

  Fragments arranged by size: 

                 880      13       7
 
PpuMI rG'GwC_Cy

Cuts at:      0     690     900 
   Size:        690     210
 
PshAI GACnn'nnGTC

Cuts at:      0     598     900 
   Size:        598     302
 
PspXI vC'TCGA_Gb

Cuts at:      0     528     900 
   Size:        528     372
 
PstI C_TGCA'G

Cuts at:      0     281     900 
   Size:        281     619
 
SfcI C'TryA_G

Cuts at:      0     277     900 
   Size:        277     623
 
SmlI C'TyrA_G

Cuts at:      0     528     900 
   Size:        528     372
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     602     634     900 
   Size:        602      32     266

  Fragments arranged by size: 

                 602     266      32
 
XhoI C'TCGA_G

Cuts at:      0     528     900 
   Size:        528     372
 
XmnI GAAnn'nnTTC

Cuts at:      0     889     900 
   Size:        889      11
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AhdI    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI     BclI Bme1580I     BmrI     BplI     BsaI    BsaAI
    BsaWI    BseYI     BsgI    BsiEI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI    BstYI     BtgI    BtgZI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRV    Hin4I     KpnI
     MmeI     MslI   MspA1I     NaeI   NgoMIV     PfoI    PpuMI    PshAI
    PspXI     PstI     SfcI     SmlI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaHI    BsaXI    BseRI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MscI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI     PsiI   PspOMI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XmaI     ZraI
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