Restriction map for pF1KB4629
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AflII       AgeI        AhdI        AlwNI       Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvrII       BalI        
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstUI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoT14I     FbaI        FseI        
HgaI        HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PvuI        RsrII       SacII       SalI        SapI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SspBI       SspI        StyI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AclI         1096  AACGTT               AA^CGTT
AcyI          624  GGCGCC               G[GA]^CG[CT]C
AflIII       1316  ACATGT               A^C[GA][CT]GT
AocI           25  CCTGAGG              CC^T[ACGT]AGG
AviII         950  TGCGCA               TGC^GCA
BanI          624  GGCGCC               G^G[CT][GA]CC
BbeI          624  GGCGCC               GGCGC^C
BglI          874  GCCATGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1304  AGATCT               A^GATCT
BsaHI         624  GGCGCC               G[GA]^CG[CT]C
BsgI         1285  CTGCAC               GTGCAG(16/14)
BsiWI         298  CGTACG               C^GTACG
BspHI        1091  TCATGA               T^CATGA
BspLU11I     1316  ACATGT               A^CATGT
BsrDI        1053  CATTGC               GCAATG(2/0)
BssSI         859  CACGAG               CACGAG(-5/-1)
BstYI        1304  AGATCT               [GA]^GATC[CT]
Bsu36I         25  CCTGAGG              CC^T[ACGT]AGG
DraII         190  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI         1102  CCACGG               C^C[GA][CT]GG
Eco81I         25  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      190  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1144  GAATTC               G^AATTC
EcoRV         976  GATATC               GAT^ATC
EcoT22I       266  ATGCAT               ATGCA^T
FspI          950  TGCGCA               TGC^GCA
HaeII         624  GGCGCC               [GA]GCGC^[CT]
Hin1I         624  GGCGCC               G[GA]^CG[CT]C
HincII        892  GTCAAC               GT[CT]^[GA]AC
HindII        892  GTCAAC               GT[CT]^[GA]AC
HinfI         348  GATTC                G^A[ACGT]TC
KasI          624  GGCGCC               G^GCGCC
MflI         1304  AGATCT               [GA]^GATC[CT]
NarI          624  GGCGCC               GG^CGCC
NsiI          266  ATGCAT               ATGCA^T
Psp1406I     1096  AACGTT               AA^CGTT
PvuII         339  CAGCTG               CAG^CTG
RcaI         1091  TCATGA               T^CATGA
SbfI         1152  CCTGCAGG             CCTGCA^GG
ScaI          377  AGTACT               AGT^ACT
SexAI         830  ACCAGGT              A^CC[AT]GGT
SfoI          624  GGCGCC               GGC^GCC
SmlI          844  CTCAAG               C^T[CT][GA]AG
SphI          102  GCATGC               GCATG^C
Sse8387I     1152  CCTGCAGG             CCTGCA^GG
TfiI          348  GATTC                G^A[AT]TC
XhoII        1304  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          997  GAATTT               [GA]^AATT[CT]
             1144  GAATTC              
ApoI          997  GAATTT               [GA]^AATT[CT]
             1144  GAATTC              
Asp700I       525  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              561  GAAAAACTTC          
BbsI         1263  GAAGAC               GAAGAC(2/6)
              733  GTCTTC              
BpmI         1297  CTCCAG               CTGGAG(16/14)
              316  CTGGAG              
BpuAI        1263  GAAGAC               GAAGAC(2/6)
              733  GTCTTC              
BsaJI        1019  CCCTGG               C^C[ACGT][ACGT]GG
             1102  CCACGG              
BsmFI          93  GGGAC                GGGAC(10/14)
              233  GGGAC               
BspMI        1151  ACCTGC               ACCTGC(4/8)
             1214  ACCTGC              
BsrFI         604  ACCGGC               [GA]^CCGG[CT]
             1079  ACCGGC              
BstAPI        365  GCAACAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1287  GCACCCTCTGC         
BstEII        834  GGTTACC              G^GT[ACGT]ACC
             1227  GGTCACC             
BstPI         834  GGTTACC              G^GT[ACGT]ACC
             1227  GGTCACC             
Cfr10I        604  ACCGGC               [GA]^CCGG[CT]
             1079  ACCGGC              
EcoO65I       834  GGTTACC              G^GT[ACGT]ACC
             1227  GGTCACC             
MaeII         731  ACGT                 A^CGT
             1097  ACGT                
StuI          527  AGGCCT               AGG^CCT
             1086  AGGCCT              
TaqI          647  TCGA                 T^CGA
             1142  TCGA                
Tsp509I       998  AATT                 ^AATT
             1145  AATT                
TthHB8I       647  TCGA                 T^CGA
             1142  TCGA                
XmnI          525  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              561  GAAAAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      AlwI        
ApaLI       AspHI       AvaII       BanII       BbvI        
BcnI        BmyI        BsaI        BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
Tsp45I      TspRI       
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