Restriction map for pF1KB4535
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        Eam1105I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MseI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tsp509I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccIII        819  TCCGGA               T^CCGGA
AflIII        200  ACGTGT               A^C[GA][CT]GT
AlwNI         322  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1077  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          949  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI          395  GCAACGTGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          667  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         819  TCCGGA               [AT]^CCGG[AT]
BseAI         819  TCCGGA               T^CCGGA
BseRI         490  GAGGAG               GAGGAG(10/8)
BsmI          519  GAATGC               GAATGC(1/-1)
BspEI         819  TCCGGA               T^CCGGA
BstXI          29  CCATGATAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         323  AGATCC               [GA]^GATC[CT]
DraIII       1123  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          254  CGGCCG               C^GGCCG
EclXI         254  CGGCCG               C^GGCCG
Eco52I        254  CGGCCG               C^GGCCG
MflI          323  AGATCC               [GA]^GATC[CT]
MroI          819  TCCGGA               T^CCGGA
NcoI         1278  CCATGG               C^CATGG
NdeI          973  CATATG               CA^TATG
PflFI         949  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         321  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          493  GAGTC                GAGTC(4/5)
SacI         1201  GAGCTC               GAGCT^C
SapI         1203  GCTCTTC              GCTCTTC(1/4)
SmaI          121  CCCGGG               CCC^GGG
SphI         1171  GCATGC               GCATG^C
TfiI          924  GATTC                G^A[AT]TC
Tth111I       949  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        321  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          368  CCAACGGTCTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         323  AGATCC               [GA]^GATC[CT]
XmaCI         121  CCCGGG               C^CCGGG
XmaI          121  CCCGGG               C^CCGGG
XmnI         1077  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          435  GTAC                 GT^AC
              997  GTAC                
AlwI          324  GATCC                GGATC(4/5)
              810  GATCC               
AocI          453  CCTCAGG              CC^T[ACGT]AGG
             1015  CCTAAGG             
Aor51HI       549  AGCGCT               AGC^GCT
              938  AGCGCT              
BalI           26  TGGCCA               TGG^CCA
               38  TGGCCA              
BanII        1183  GAGCCC               G[GA]GC[CT]^C
             1201  GAGCTC              
BfaI            7  CTAG                 C^TAG
              469  CTAG                
BsaI          653  GAGACC               GGTCTC(1/5)
              373  GGTCTC              
BsiEI         254  CGGCCG               CG[GA][CT]^CG
              297  CGACCG              
BsmAI         653  GAGAC                GTCTC(1/5)
              374  GTCTC               
BspHI         806  TCATGA               T^CATGA
              927  TCATGA              
BsrFI          71  ACCGGC               [GA]^CCGG[CT]
              228  GCCGGT              
Bsu36I        453  CCTCAGG              CC^T[ACGT]AGG
             1015  CCTAAGG             
Cfr10I         71  ACCGGC               [GA]^CCGG[CT]
              228  GCCGGT              
DsaI          279  CCACGG               C^C[GA][CT]GG
             1278  CCATGG              
EarI         1204  CTCTTC               CTCTTC(1/4)
             1210  CTCTTC              
Eco47III      549  AGCGCT               AGC^GCT
              938  AGCGCT              
Eco81I        453  CCTCAGG              CC^T[ACGT]AGG
             1015  CCTAAGG             
EcoNI         468  CCTAGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              657  CCTGACTGAGG         
HgaI          225  GACGC                GACGC(5/10)
              353  GACGC               
HinfI         493  GAGTC                G^A[ACGT]TC
              924  GATTC               
Ksp632I      1204  CTCTTC               CTCTTC(1/4)
             1210  CTCTTC              
MaeI            7  CTAG                 C^TAG
              469  CTAG                
MluNI          26  TGGCCA               TGG^CCA
               38  TGGCCA              
MscI           26  TGGCCA               TGG^CCA
               38  TGGCCA              
MspA1I        214  CAGCTG               C[AC]G^C[GT]G
              595  CAGCTG              
PpuMI         563  AGGTCCT              [GA]G^G[AT]CC[CT]
              632  GGGACCC             
PstI          211  CTGCAG               CTGCA^G
             1228  CTGCAG              
PvuII         214  CAGCTG               CAG^CTG
              595  CAGCTG              
RcaI          806  TCATGA               T^CATGA
              927  TCATGA              
RsaI          435  GTAC                 GT^AC
              997  GTAC                
SfcI          211  CTGCAG               C^T[GA][CT]AG
             1228  CTGCAG              
SgrAI          70  CACCGGCG             C[GA]^CCGG[CT]G
              227  CGCCGGTG            
StuI          665  AGGCCT               AGG^CCT
             1139  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaI        AvaII       BanI        BbeI        BbvI        
BcnI        BmyI        BsaHI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        KasI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       NspI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfoI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
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