Restriction map for pF1KB4184
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        Alw44I      AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       BcgI        BfrI        
BsaBI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HgaI        
HindIII     HpaI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     Van91I      XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccIII        908  TCCGGA               T^CCGGA
AcsI          139  GAATTC               [GA]^AATT[CT]
AcyI          785  GGCGCC               G[GA]^CG[CT]C
AhdI          527  GACAGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          139  GAATTC               [GA]^AATT[CT]
AspEI         527  GACAGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          601  CCCGGG               C^[CT]CG[GA]G
AvrII         552  CCTAGG               C^CTAGG
BbeI          785  GGCGCC               GGCGC^C
BbrPI        1458  CACGTG               CAC^GTG
BbsI          389  GAAGAC               GAAGAC(2/6)
BbvCI        1149  CCTCAGC              CCTCAGC(-5/-2)
BciVI         228  GTATCC               GTATCC(6/5)
BclI          377  TGATCA               T^GATCA
BglI          682  GCCTGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1406  AGATCT               A^GATCT
BlnI          552  CCTAGG               C^CTAGG
BlpI          491  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      491  GCTCAGC              GC^T[ACGT]AGC
BpuAI         389  GAAGAC               GAAGAC(2/6)
BsaAI        1458  CACGTG               [CT]AC^GT[GA]
BsaHI         785  GGCGCC               G[GA]^CG[CT]C
BsaI         1381  GAGACC               GGTCTC(1/5)
BsaWI         908  TCCGGA               [AT]^CCGG[AT]
BseAI         908  TCCGGA               T^CCGGA
BsoBI         601  CCCGGG               C^[CT]CG[GA]G
BspEI         908  TCCGGA               T^CCGGA
BsrFI          67  GCCGGC               [GA]^CCGG[CT]
BssHII        756  GCGCGC               G^CGCGC
CelII         491  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         67  GCCGGC               [GA]^CCGG[CT]
Eam1105I      527  GACAGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         139  GAATTC               G^AATTC
FbaI          377  TGATCA               T^GATCA
Hin1I         785  GGCGCC               G[GA]^CG[CT]C
HincII       1485  GTCAAC               GT[CT]^[GA]AC
HindII       1485  GTCAAC               GT[CT]^[GA]AC
KasI          785  GGCGCC               G^GCGCC
MaeII        1459  ACGT                 A^CGT
MroI          908  TCCGGA               T^CCGGA
MslI          315  CATCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        588  CAGCTG               C[AC]G^C[GT]G
NaeI           67  GCCGGC               GCC^GGC
NarI          785  GGCGCC               GG^CGCC
NgoMIV         67  GCCGGC               G^CCGGC
PmaCI        1458  CACGTG               CAC^GTG
PmlI         1458  CACGTG               CAC^GTG
PpuMI         264  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI         1090  CTGCAG               CTGCA^G
PvuII         588  CAGCTG               CAG^CTG
SfcI         1090  CTGCAG               C^T[GA][CT]AG
SfoI          785  GGCGCC               GGC^GCC
SmaI          601  CCCGGG               CCC^GGG
TaqI         1004  TCGA                 T^CGA
TthHB8I      1004  TCGA                 T^CGA
XbaI           22  TCTAGA               T^CTAGA
XmaCI         601  CCCGGG               C^CCGGG
XmaI          601  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AvaII         209  GGTCC                G^G[AT]CC
              265  GGTCC               
BamHI          41  GGATCC               G^GATCC
             1184  GGATCC              
BanI          597  GGTGCC               G^G[CT][GA]CC
              785  GGCGCC              
BssSI         397  CTCGTG               CACGAG(-5/-1)
              838  CTCGTG              
BstAPI        253  GCACTGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1232  GCATGGTCTGC         
MaeIII        829  GTGAC                ^GT[ACGT]AC
             1177  GTGAC               
TfiI           30  GATTC                G^A[AT]TC
              347  GATTC               
Tsp45I        829  GTGAC                ^GT[GC]AC
             1177  GTGAC               
Tsp509I       140  AATT                 ^AATT
             1418  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AocI        ApaI        AspHI       BalI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MboI        MboII       MflI        
MluNI       MnlI        MscI        MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       StuI        
StyI        TseI        TspRI       XhoII       
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