Restriction map for pF1KB4184
 (Linear) (Six-base) MAPSORT of: KIBB4184.seq  Check: 3794  from: 1  to: 1497



KIBB4184.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     403    1497 
   Size:        403    1094
 
AhdI GACnn_n'nnGTC

Cuts at:      0     532    1497 
   Size:        532     965
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      61    1497 
   Size:         61    1436
 
ApaI G_GGCC'C

Cuts at:      0     816    1449    1456    1497 
   Size:        816     633       7      41

  Fragments arranged by size: 

                 816     633      41       7
 
ApoI r'AATT_y

Cuts at:      0     139    1497 
   Size:        139    1358
 
AvaI C'yCGr_G

Cuts at:      0     601    1497 
   Size:        601     896
 
AvrII C'CTAG_G

Cuts at:      0     552    1497 
   Size:        552     945
 
BamHI G'GATC_C

Cuts at:      0      41    1184    1497 
   Size:         41    1143     313

  Fragments arranged by size: 

                1143     313      41
 
BanI G'GyrC_C

Cuts at:      0     597     785    1497 
   Size:        597     188     712

  Fragments arranged by size: 

                 712     597     188
 
BanII G_rGCy'C

Cuts at:      0     470     493     551     715     816     866    1099    1449 
   Size:        470      23      58     164     101      50     233     350

Cuts at:   1449    1456    1497 
   Size:          7      41

  Fragments arranged by size: 

                 470     350     233     164     101      58      50      41
                  23       7
 
BbeI G_GCGC'C

Cuts at:      0     789    1497 
   Size:        789     708
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     396    1497 
   Size:        396    1101
 
BbvCI CC'TCA_GC

Cuts at:      0    1150    1497 
   Size:       1150     347
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     239    1497 
   Size:        239    1258
 
BclI T'GATC_A

Cuts at:      0     377    1497 
   Size:        377    1120
 
BglI GCCn_nnn'nGGC

Cuts at:      0     688    1497 
   Size:        688     809
 
BglII A'GATC_T

Cuts at:      0    1406    1497 
   Size:       1406      91
 
BlpI GC'TnA_GC

Cuts at:      0     492    1497 
   Size:        492    1005
 
Bme1580I G_kGCm'C

Cuts at:      0     602     783     816    1449    1456    1497 
   Size:        602     181      33     633       7      41

  Fragments arranged by size: 

                 633     602     181      41      33       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     968    1497 
   Size:        968     529
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     595     757     766    1492    1497 
   Size:        191     404     162       9     726       5

  Fragments arranged by size: 

                 726     404     191     162       9       5
 
Bpu10I CC'TnA_GC

Cuts at:      0    1150    1497 
   Size:       1150     347
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1375    1497 
   Size:       1375     122
 
BsaAI yAC'GTr

Cuts at:      0    1460    1497 
   Size:       1460      37
 
BsaHI Gr'CG_yC

Cuts at:      0     786    1497 
   Size:        786     711
 
BsaWI w'CCGG_w

Cuts at:      0     908    1497 
   Size:        908     589
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     689     719     883     913    1291    1321    1497 
   Size:        689      30     164      30     378      30     176

  Fragments arranged by size: 

                 689     378     176     164      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     488     646     754     763     766     834     888    1479 
   Size:        488     158     108       9       3      68      54     591

Cuts at:   1479    1497 
   Size:         18

  Fragments arranged by size: 

                 591     488     158     108      68      54      18       9
                   3
 
BseYI C'CCAG_C

Cuts at:      0     282     384    1058    1497 
   Size:        282     102     674     439

  Fragments arranged by size: 

                 674     439     282     102
 
BsiHKAI G_wGCw'C

Cuts at:      0     255     470     493     551     866    1099    1497 
   Size:        255     215      23      58     315     233     398

  Fragments arranged by size: 

                 398     315     255     233     215      58      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     255     470     493     551     602     715     783     816 
   Size:        255     215      23      58      51     113      68      33

Cuts at:    816     866    1099    1449    1456    1497 
   Size:         50     233     350       7      41

  Fragments arranged by size: 

                 350     255     233     215     113      68      58      51
                  50      41      33      23       7
 
BspEI T'CCGG_A

Cuts at:      0     908    1497 
   Size:        908     589
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     253     403    1063    1497 
   Size:        253     150     660     434

  Fragments arranged by size: 

                 660     434     253     150
 
BsrFI r'CCGG_y

Cuts at:      0      67    1497 
   Size:         67    1430
 
BssHII G'CGCG_C

Cuts at:      0     756    1497 
   Size:        756     741
 
BssSI C'ACGA_G

Cuts at:      0     397     838    1497 
   Size:        397     441     659

  Fragments arranged by size: 

                 659     441     397
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     259    1238    1497 
   Size:        259     979     259

  Fragments arranged by size: 

                 979     259     259
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     282     444     594    1497 
   Size:        282     162     150     903

  Fragments arranged by size: 

                 903     282     162     150
 
BstYI r'GATC_y

Cuts at:      0      41     935    1184    1406    1497 
   Size:         41     894     249     222      91

  Fragments arranged by size: 

                 894     249     222      91      41
 
Bsu36I CC'TnA_GG

Cuts at:      0     461     563     817    1497 
   Size:        461     102     254     680

  Fragments arranged by size: 

                 680     461     254     102
 
BtgI C'CryG_G

Cuts at:      0     790     857    1124    1497 
   Size:        790      67     267     373

  Fragments arranged by size: 

                 790     373     267      67
 
BtsI GCAGTG_nn'

Cuts at:      0     178     253     858    1497 
   Size:        178      75     605     639

  Fragments arranged by size: 

                 639     605     178      75
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     821     856    1497 
   Size:        821      35     641

  Fragments arranged by size: 

                 821     641      35
 
EaeI y'GGCC_r

Cuts at:      0     272     482     584    1121    1190    1214    1497 
   Size:        272     210     102     537      69      24     283

  Fragments arranged by size: 

                 537     283     272     210     102      69      24
 
EcoICRI GAG'CTC

Cuts at:      0     468     491     549     864    1097    1497 
   Size:        468      23      58     315     233     400

  Fragments arranged by size: 

                 468     400     315     233      58      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     595     757     766    1492    1497 
   Size:        191     404     162       9     726       5

  Fragments arranged by size: 

                 726     404     191     162       9       5
 
EcoO109I rG'GnC_Cy

Cuts at:      0     265     813    1452    1453    1497 
   Size:        265     548     639       1      44

  Fragments arranged by size: 

                 639     548     265      44       1
 
EcoRI G'AATT_C

Cuts at:      0     139    1497 
   Size:        139    1358
 
HaeII r_GCGC'y

Cuts at:      0     458     610     789    1249    1497 
   Size:        458     152     179     460     248

  Fragments arranged by size: 

                 460     458     248     179     152
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     518     550     999    1031    1291    1323    1497 
   Size:        518      32     449      32     260      32     174

  Fragments arranged by size: 

                 518     449     260     174      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1487    1497 
   Size:       1487      10
 
KasI G'GCGC_C

Cuts at:      0     785    1497 
   Size:        785     712
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     516    1497 
   Size:        516     981
 
MscI TGG'CCA

Cuts at:      0     274     484     586    1123    1192    1216    1497 
   Size:        274     210     102     537      69      24     281

  Fragments arranged by size: 

                 537     281     274     210     102      69      24
 
MslI CAynn'nnrTG

Cuts at:      0     319    1497 
   Size:        319    1178
 
MspA1I CmG'CkG

Cuts at:      0     590    1497 
   Size:        590     907
 
NaeI GCC'GGC

Cuts at:      0      69    1497 
   Size:         69    1428
 
NarI GG'CG_CC

Cuts at:      0     786    1497 
   Size:        786     711
 
NcoI C'CATG_G

Cuts at:      0     790     857    1124    1497 
   Size:        790      67     267     373

  Fragments arranged by size: 

                 790     373     267      67
 
NgoMIV G'CCGG_C

Cuts at:      0      67    1497 
   Size:         67    1430
 
PasI CC'CwG_GG

Cuts at:      0     234    1449    1497 
   Size:        234    1215      48

  Fragments arranged by size: 

                1215     234      48
 
PmlI CAC'GTG

Cuts at:      0    1460    1497 
   Size:       1460      37
 
PpuMI rG'GwC_Cy

Cuts at:      0     265    1497 
   Size:        265    1232
 
PspOMI G'GGCC_C

Cuts at:      0     812    1445    1452    1497 
   Size:        812     633       7      45

  Fragments arranged by size: 

                 812     633      45       7
 
PstI C_TGCA'G

Cuts at:      0    1094    1497 
   Size:       1094     403
 
PvuII CAG'CTG

Cuts at:      0     590    1497 
   Size:        590     907
 
SacI G_AGCT'C

Cuts at:      0     470     493     551     866    1099    1497 
   Size:        470      23      58     315     233     398

  Fragments arranged by size: 

                 470     398     315     233      58      23
 
SfcI C'TryA_G

Cuts at:      0    1090    1497 
   Size:       1090     407
 
SfoI GGC'GCC

Cuts at:      0     787    1497 
   Size:        787     710
 
SmaI CCC'GGG

Cuts at:      0     603    1497 
   Size:        603     894
 
StuI AGG'CCT

Cuts at:      0     242     568     682    1221    1303    1497 
   Size:        242     326     114     539      82     194

  Fragments arranged by size: 

                 539     326     242     194     114      82
 
StyI C'CwwG_G

Cuts at:      0     269     552     790     857    1124    1332    1412    1497 
   Size:        269     283     238      67     267     208      80      85

  Fragments arranged by size: 

                 283     269     267     238     208      85      80      67
            
 
XbaI T'CTAG_A

Cuts at:      0      22    1497 
   Size:         22    1475
 
XmaI C'CCGG_G

Cuts at:      0     601    1497 
   Size:        601     896
 

 Enzymes that do cut:

     AarI     AhdI     AlfI     ApaI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI    CspCI     EaeI  EcoICRI  Eco57MI EcoO109I    EcoRI
    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     PasI     PmlI    PpuMI   PspOMI
     PstI    PvuII     SacI     SfcI     SfoI     SmaI     StuI     StyI
     XbaI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     BaeI     BcgI    BfrBI    BmgBI     BmtI     BplI    BpuEI    BsaBI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI
    BsrGI    BstBI   BstEII  BstZ17I    BtgZI     ClaI     DraI   DraIII
     DrdI     EagI     EarI     EciI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmnI     ZraI
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